Antibody-Conjugated Gold Nanoparticle-Based Immunosensor for Ultra-Sensitive Detection of Troponin-T

Jacobs, Michael; Selvam, Anjan Panneer; Craven, Jon Engel; Prasad, Shalini

doi:10.1177/2211068214538971

Cited by 28 publications

(22 citation statements)

References 22 publications

(28 reference statements)

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“…In our model we used the well-described [15][16][17] cross-linker molecule DSP to conjugate the probe antibody to the electrode surface. We characterized the assembly of the antibody onto the working electrode using optical and electrochemical methods.…”

Section: Antibody Complex Is Assembled Onto An Electrodementioning

confidence: 99%

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

et al. 2020

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Electrochemical sensors based on antibody-antigen recognition events are commonly used for the rapid, label-free, and sensitive detection of various analytes. However, various parameters at the bioelectronic interface, i.e., before and after the probe (such as an antibody) assembly onto the electrode, have a dominant influence on the underlying detection performance of analytes (such as an antigen). In this work, we thoroughly investigate the dependence of the bioelectronic interface characteristics on parameters that have not been investigated in depth: the antibody density on the electrode's surface and the antigen incubation time. For this important aim, we utilized the sensitive non-faradaic electrochemical impedance spectroscopy method. We showed that as the incubation time of the antigen-containing drop solution increased, a decrease was observed in both the solution resistance and the diffusional resistance with reflecting boundary elements, as well as the capacitive magnitude of a constant phase element, which decreased at a rate of 160 ± 30 kΩ/min, 800 ± 100 mΩ/min, and 520 ± 80 pF × s (α-1) /min, respectively. Using atomic force microscopy, we also showed that high antibody density led to thicker electrode coating than low antibody density, with rootmean-square roughness values of 2.2 ± 0.2 nm versus 1.28 ± 0.04 nm, respectively. Furthermore, we showed that as the antigen accumulated onto the electrode, the solution resistance increased for high antibody density and decreased for low antibody density. Finally, the antigen detection performance test yielded a better limit of detection for low antibody density than for high antibody density (0.26 μM vs 2.2 μM). Overall, we show here the importance of these two factors and how changing one parameter can drastically affect the desired outcome.

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Section: Antibody Complex Is Assembled Onto An Electrodementioning

confidence: 99%

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

et al. 2020

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“…Although Karimian et al (2013) has reported an ultrasensitive cTnT MIP by employing the poly-o-phenylenediamine polymer with similar LOD achieved in this work, their biomimetic sensor consisted of a conventional gold electrode, still requiring progress to become a point-of-care testing. As compared with cTnT immunosensor using cTnT monoclonal antibodies (Dutra et al, 2007;Jacobs et al, 2014;Silva et al, 2013), this N-MIP exhibited a comparable linear range and the LOD (0.006 ng mL À 1 ) was found in same order of magnitude.…”

Section: Analytical Performance Of the N-mip Sensormentioning

confidence: 90%

An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer

Silva

Rodríguez

Sales

et al. 2016

Biosensors and Bioelectronics

108

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A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ngmL(-1) (r=0.995, p«0.01), with a very low limit of detection (0.006 ngmL(-1)). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (KD=7.3 10(-13) molL(-1)). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.

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“…In this present study we have developed an electrochemical ELISA where electronic signals were used for detection. Signal enhancement by bio-functional nanomaterials like Gold nanoparticles and quantum dots are used frequently due to their semiconductor and biocompatible properties [35][36][37]. Gold nanoparticles (AuNPs) and QDs can provide a natural environment for bimolecular immobilization and facilitate the electron-transfer because of their high surface area and electrochemistry [38][39][40].…”

Section: Discussionmentioning

confidence: 99%

Impedimetric detection of Banana bunchy top virus using CdSe quantum dots for signal amplification

et al. 2020

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Banana bunchy top virus is considered to be the most economically destructive pathogens of banana that causes severe economic loss in banana plantations worldwide, including India. In this present study we have developed an improved electrochemical ELISA for detection of Banana bunchy top virus (BBTV). For enhanced and accurate detection we have used cadmium selenide (CdSe) quantum dots (QDs) as signal amplifiers. Experiments in this study were performed using primary antibody raised from recombinant coat protein of BBTV. CdSe QDs could significantly amplify the electrical signals in this assay and make the method appropriate for lab use. The result of electrical conduction showed the difference of impedance between the healthy and diseased sample of the order of ~ 100 Ω. The electrochemical ELISA could detect the virus in plant sap up to dilution of 1:25 as compared to 1:10 of conventional ELISA.

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Antibody-Conjugated Gold Nanoparticle-Based Immunosensor for Ultra-Sensitive Detection of Troponin-T

Cited by 28 publications

References 22 publications

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer

Impedimetric detection of Banana bunchy top virus using CdSe quantum dots for signal amplification

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