An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer

Abstract

A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ngmL(-1) (r=0.995, p«0.01), with a very low limit of detection (0.006 ngmL(-1)). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (KD=7.3 10(-13) molL(-1)). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.

Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

“…[12][13][14] While the molecular imprinting concept is fairly universal the functional monomers to provide selective recognition are not, i.e., the library of monomers is rather limited and monomers adequate for a certain protein target may not be necessarily optimal for another. [15] While high affinity MIPs were reported for a number of targets [16,17], the selectivity of protein-MIPs is often enhanced by incorporating in the MIPs compounds known to interact with the target, e.g. substrates or inhibitors of an enzyme target [18,19], aptamers [20] and various nanomaterials [16,21].…”

Molecularly imprinted electrochemical sensor prepared on a screen printed carbon electrode for naloxone detection

“…The SPEs are very versatile since the composition of the printing inks could be modified with several substances such as metals, enzymes, polymers or complexing agents [17]. Otherwise deposition of different agents on the surfaces of manufactured electrodes could also be easily achieved such as molecular imprinted polymers [17][18][19].…”

Nanomaterials‐based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress

“…TnI and TnT are absent in the blood of healthy people because they are released into the bloodstream due to death of the myocardial cells and this makes them important biomarkers for AMI diagnosis [6]. The cTnT is one of the well-known cardiac protein markers and is released rapidly of the contractile apparatus in cardiac muscle within 2-4 h after AMI symptoms appearance and can be kept elevated for up to 14 days, thus it is regarded as a specific and sensitive biomarker for myocardial injury diagnosis [2,48]. On the other hand, the cTnI is considered as gold standard for the diagnosis of AMI not only because it is absent in skeletal muscles, but also its concentration in the bloodstream increases and remains elevated for 8 to 10 days, providing longer window of detection for myocardial infarction [4][5].…”