Antibody capture radioimmunoassay for anti-rubella IgM

Mortimer, Philip P.; Tedder, Richard S.; Hambling, M.H.; Shafi, Shuja; Burkhardt, Felix; Schilt, U

doi:10.1017/s0022172400068856

Cited by 84 publications

(40 citation statements)

References 13 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eight of the sera contained specific IgM detectable at a concentration greater than 3 a.u. by IgM antibody-capture radioimmunoassay MACRIA (Mortimer et al 1981).…”

Section: Seramentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYAn antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1 and IgG3 was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG, or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value.Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1 the DEA shift value was < 0W6 for cases of rubella in the distant past, compared with > 0-8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0-65.No serum from cases of rubella in the distant past contained sufficient specific IgG3 to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values > 0 7 compared with sera from reinfections, which gave DEA shift values < 0 6, except for two sera from a case of symptomatic reinfection.Thus the assessment of specific IgG subclass avidity is of value in differentiating serologically primary rubella from reinfection.

show abstract

“…Eight of the sera contained specific IgM detectable at a concentration greater than 3 a.u. by IgM antibody-capture radioimmunoassay MACRIA (Mortimer et al 1981).…”

Section: Seramentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…We would suggest that the rubella-specific IgM control sera as used by Mortimer et al (1981) should be internationally accepted. Thus appropriate comparisons could be made into sensitivity and specificity of tests for rubella-specific IgM, including those becoming commercially available.…”

Section: Discussionmentioning

confidence: 99%

“…The MACRIA method employed in this evaluation was adapted from that described by Mortimer et al (1981). Polystyrene beads, 6-4 mm diameter, made by the Plastic Ball Co., Chicago, U.S.A. and supplied by Northumbria Biologicals Ltd, U.K., were used as the solid phase.…”

Section: Macriamentioning

confidence: 99%

“…This principle has been applied to the detection of rubella-specific IgM by Mortimer et al (1981) who detected any rubella-specific IgM, within the total IgM retained by the anti-,t, by sequential addition of rubella haemagglutinating antigen (HA) and J125-labelled anti-rubella IgG. This M-antibody capture radioimmunoassay (MACRIA) is a sensitive technique which allows large numbers of sera to be tested, but does require the preparation of radio-labelled anti-rubella IgG.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The detection of rubella-specific IgM by an immunosorbent assay with solid-phase attachment of red cells (SPARC)

Sexton

Hodgson

Morgan‐Capner

1982

J. Hyg.

View full text Add to dashboard Cite

SUMMARYAn immunosorbent assay using solid-phase attachment of red cells (SPARC) was used for the detection of rubella-specific IgM. The method is described and the results compared with those obtained by the IgM antibody capture radioimmunoassay (MACRIA). One hundred and ninety-nine sera were investigated for the presence of rubella-specific IgM and only one discrepant result occurred, namely a false positive obtained by MACRIA in a patient with infectious mononucleosis. Rheumatoid factor, heterophile antibody, and rubella-specific IgG did not interfere with the results obtained by the SPARC technique. Advantages of the SPARC technique include the ease and lack of expense of testing large numbers of sera, the small volume ofsample required and the fact that pretreatment of serum is not necessary.

show abstract

“…IgM capture radioimmunoassay (MACRIA), RH, and rubella-specific IgG subclass ELISAs were performed as described (3,8,9). Samples of some sera were too small for examination by all the tests.…”

Section: Serological Assaysmentioning

confidence: 99%

Laboratory diagnosis and clinical significance of rubella in children with cancer

et al. 1989

View full text Add to dashboard Cite

SUMMARYVirus-specific antibody responses were studied in 12 children with cancer in whom rubella was diagnosed by seroconversion or a rising titre (>s fourfold) of haemagglutination inhibiting (HI) antibody. Our results confirmed the difficulties of making a diagnosis of rubella infection in immunocompromised children using criteria for interpreting antibody assays established in immunocompetent patients. Specific IgM antibody persisted for more than 2 months in 7 of 10 children with probable primary rubella, 3 of whom had high concentrations of such antibody 6, 7 and 11 months after the rash. Radial haemolysis and specific IgG, and IgG3 antibody responses were low in 4, 2, and 4 patients, respectively.

show abstract

Antibody capture radioimmunoassay for anti-rubella IgM

Cited by 84 publications

References 13 publications

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

The detection of rubella-specific IgM by an immunosorbent assay with solid-phase attachment of red cells (SPARC)

Laboratory diagnosis and clinical significance of rubella in children with cancer

Contact Info

Product

Resources

About