Antibody-based biosensors for environmental monitoring

Kang, Ji-Hoon; Kim, Soyoun; Kwon, Youngeun

doi:10.1007/bf03216477

Cited by 11 publications

(3 citation statements)

References 27 publications

(23 reference statements)

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“…The basic difference between sensors and indicators is that sensors consist of receptors and transducers, whereas indicators are designed considerably simpler and provide qualitative information through immediate visual changes. On the other hand, receptors in biosensors include enzymes, hormones, antibodies, antigens, microorganisms, drugs, and nucleic acids, which can be utilized alone or in combination (Kang et al., 2009; Kerry & Butler, 2008; Sekhon et al., 2014).…”

Section: Smp: An Overviewmentioning

confidence: 99%

Application of smart packaging for seafood: A comprehensive review

Abedi‐Firoozjah

Salim

Hasanvand

et al. 2023

Comp Rev Food Sci Food Safe

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Nowadays, due to the changes in lifestyle and great interest of consumers in a healthy life, people have started increasing their seafood consumption. But due to their short shelf life, experts are looking for a new packaging called smart packaging (SMP) for seafood. There are different indicators/sensors in SMP; one of the effective indices is time–temperature, which can show consumers the best time of using seafood based on their shelf life and experienced temperature. Another one is radio‐frequency identification (RFID) that is a transmission device that represents a separate form of the electronic information‐based SMP systems. RFID does not belong to any of the categories of markers or sensors; it is an auto recognition system that applies cordless sensors to indicate segments and collect real‐time information without manual interposition. This review covers the use of SMP in all marine foods, including fish, due to its high consumption and high content of polyunsaturated fatty acids, eicosapentaenoic acid (C20:5n‐3) and docosahexaenoic acid (C22:6n‐3), which are the considerable factors of n‐3 polyunsaturated fatty acids for human.

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Section: Smp: An Overviewmentioning

confidence: 99%

Application of smart packaging for seafood: A comprehensive review

Abedi‐Firoozjah

Salim

Hasanvand

et al. 2023

Comp Rev Food Sci Food Safe

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“…In the recent years, innovative biosensor developments have made several applications with rapid detection and high accuracy possible for real-time environmental monitoring, disease diagnostics and therapy [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. During the last decade, protein-based—or more particularly antibody-based—biosensors are considered as the most commonly developed platforms [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biosensor Platforms for Detecting the Atherosclerotic Biomarker VCAM1 Based on Bioconjugation with Uniformly Oriented VCAM1-Targeting Nanobodies

Guedens

Vranken

et al. 2016

Biosensors

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Surface bioconjugation of biomolecules has gained enormous attention for developing advanced biomaterials including biosensors. While conventional immobilization (by physisorption or covalent couplings using the functional groups of the endogenous amino acids) usually results in surfaces with low activity, reproducibility and reusability, the application of methods that allow for a covalent and uniformly oriented coupling can circumvent these limitations. In this study, the nanobody targeting Vascular Cell Adhesion Molecule-1 (NbVCAM1), an atherosclerotic biomarker, is engineered with a C-terminal alkyne function via Expressed Protein Ligation (EPL). Conjugation of this nanobody to azidified silicon wafers and Biacore™ C1 sensor chips is achieved via Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) “click” chemistry to detect VCAM1 binding via ellipsometry and surface plasmon resonance (SPR), respectively. The resulting surfaces, covered with uniformly oriented nanobodies, clearly show an increased antigen binding affinity, sensitivity, detection limit, quantitation limit and reusability as compared to surfaces prepared by random conjugation. These findings demonstrate the added value of a combined EPL and CuAAC approach as it results in strong control over the surface orientation of the nanobodies and an improved detecting power of their targets—a must for the development of advanced miniaturized, multi-biomarker biosensor platforms.

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“…Antibody-based biosensors have proved to be sensitive and rapid tools for the detection of a wide range of pathogens and toxins in environmental monitoring 15,16,17,18,19,20,21 . While DNA methods involve several steps, the antibody-based microarrays only require a small sample preparation that is mainly based on a short lysis step in an appropriate solution buffer.…”

mentioning

confidence: 99%

Experimental Protocol for Detecting <em>Cyanobacteria </em>in Liquid and Solid Samples with an Antibody Microarray Chip

Blanco¹,

Moreno‐Paz²,

Parro³

2017

JoVE

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Global warming and eutrophication make some aquatic ecosystems behave as true bioreactors that trigger rapid and massive cyanobacterial growth; this has relevant health and economic consequences. Many cyanobacterial strains are toxin producers, and only a few cells are necessary to induce irreparable damage to the environment. Therefore, water-body authorities and administrations require rapid and efficient early-warning systems providing reliable data to support their preventive or curative decisions. This manuscript reports an experimental protocol for the in-field detection of toxin-producing cyanobacterial strains by using an antibody microarray chip with 17 antibodies (Abs) with taxonomic resolution (CYANOCHIP). Here, a multiplex fluorescent sandwich microarray immunoassay (FSMI) for the simultaneous monitoring of 17 cyanobacterial strains frequently found blooming in freshwater ecosystems, some of them toxin producers, is described. A microarray with multiple identical replicates (up to 24) of the CYANOCHIP was printed onto a single microscope slide to simultaneously test a similar number of samples. Liquid samples can be tested either by direct incubation with the antibodies (Abs) or after cell concentration by filtration through a 1- to 3-μm filter. Solid samples, such as sediments and ground rocks, are first homogenized and dispersed by a hand-held ultrasonicator in an incubation buffer. They are then filtered (5 - 20 μm) to remove the coarse material, and the filtrate is incubated with Abs. Immunoreactions are revealed by a final incubation with a mixture of the 17 fluorescence-labeled Abs and are read by a portable fluorescence detector. The whole process takes around 3 h, most of it corresponding to two 1-h periods of incubation. The output is an image, where bright spots correspond to the positive detection of cyanobacterial markers.

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Antibody-based biosensors for environmental monitoring

Cited by 11 publications

References 27 publications

Application of smart packaging for seafood: A comprehensive review

Application of smart packaging for seafood: A comprehensive review

Enhanced Biosensor Platforms for Detecting the Atherosclerotic Biomarker VCAM1 Based on Bioconjugation with Uniformly Oriented VCAM1-Targeting Nanobodies

Experimental Protocol for Detecting <em>Cyanobacteria </em>in Liquid and Solid Samples with an Antibody Microarray Chip

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