Antibodies to α-fetoprotein disrupt histogenesis in cultured chick retinae

Palazón, Luis Sánchez; Copado, M. A.; Ruı́z-Gutı́errez, Valentina; Dorado, Manuel E.; Burgos, A. Rodríguez

doi:10.1016/s0040-8166(96)80010-3

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Little is known about the role of AFP in the eye, although the essential role of AFP in the normal development of retina has been reported. 29 Rather, a possible indirect role of AFP contributing to the progress of diabetic retinopathy should be subject to further investigations, considering a reported correlation between AFP and HbA1c. 14 In this study, mean (TSD) age at menopause was 49.2 (T3.3) years and mean (TSD) body mass index was 25.4 (T5.4) kg/m 2 , which is consistent with those of the healthy Korean population as we reported previously.…”

Section: Discussionmentioning

confidence: 99%

Sex steroid and tropic hormone levels may be associated with postoperative prognosis of vitrectomy in Korean postmenopausal women

Choi³

et al. 2010

Menopause

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Section: Discussionmentioning

confidence: 99%

Sex steroid and tropic hormone levels may be associated with postoperative prognosis of vitrectomy in Korean postmenopausal women

Choi³

et al. 2010

Menopause

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“…Studies of the role of AFP in eye development have indicated that antibodies to AFP are capable of disrupting eye histogenesis in chick retinae cultures ( 159). Seventeen-day retina cultures treated with antibodies to chicken AFP in organ culture for 3 days failed to form both the plexiform layers and photoreceptor cell buds.…”

Section: Oncogene Transcription and Expressionmentioning

confidence: 99%

-Fetoprotein as a Biologic Response Modifier: Relevance to Domain and Subdomain Structure

1997

Experimental Biology and Medicine

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In the present review, the structure of alpha-fetoprotein (AFP) is discussed in consideration of AFP membership and position in the albuminoid supergene family in relation to other gene family members. Ontogenetic AFP gene expression is then discussed in view of AFP mRNA presence in various tissues at different times during development. The multiple molecular forms of AFP is also presented in relation to published reports of AFP binding proteins and cell surface receptors. The review proceeds on to present AFP as a potential model of a modular/cassette protein based on sequence comparison with cleaved fragments of prohormones and biological response modifiers. Such cleaved fragments could potentially serve as peptide messengers for vascular, neuroendocrine, and digestive biological activities. Following a discussion on fibrin binding and serine proteases, AFP-cytoskeletal, extracellular matrix, and cellular adhesion interactions are considered. AFP as a carrier/transport protein based on structural relationships is further elucidated by examination of the various ligands bound to AFP and its hormone interaction. Since AFP binds heavy metals, the question is posed of whether AFP could function as an antioxidant. An analysis of transcription factors, tumor suppressors, and homeodomain proteins follows, which is interfaced with the concept of programmed cell death in light of amino acid sequence matches detected on the AFP molecule. Emphasis was naturally placed upon the homeodomain protein sequence stretches since AFP is a fetal, phase-specific protein found throughout embryogenesis, histogenesis, and organogenesis. In keeping with histogenesis, a discussion of AFP and eye lens protein development is presented. Finally, AFP sequence analysis presented in light of members of the immunoglobulin superfamily, autoimmune disorders, and various disease states culminates the review. A closing discussion then summarizes regions of presumptive matched protein identities on each of AFP's three domains.

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“…Neutralizing α-fetoprotein was found to give rise to congenital malformations, arrested development and fetal death [14]. Also, incorporating α-fetoprotein antiserum to the culture medium was found to disrupt histological development of the retina [15]. …”

Section: Introductionmentioning

confidence: 99%

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

Rodriguez-Burgos

2003

BMC Immunol

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BackgroundThe aim of this work was to detect antigens, non-self to the dam, potentially present in chick embryo prior to organogenesis with a view to establishing the consequences of their neutralization on chick development. To this end, hens were immunized with the extract from embryos incubated for 53 h. Their eggs were either used to isolate immunoglobulins for dot and blot tests or incubated for variable lengths of time.ResultsImmunoblot tests, using adsorbed primary and secondary antibodies against paternal serum, revealed the presence of at least four antigens of 32, 34, 70 and 200 kDa that can be classified as soluble alloantigens. The same antibodies against chick embryo extracts (between 53 h and 9) showed at least five aged antigens of 34, 52, 90, 200 and 250 kDa, not detected in cock serum, that can thus be considered as soluble, foreign to the immunized hens and transitory antigens. The abnormalities observed included arrested development and fetal death, as well as minor functional damage in the few chicks that were born alive. The ratio of abnormal to normal embryos was 2.85 in the experimental group and 0.43 in the control group. With regard to congenital anomalies it must be said that of the 81 eggs incubated only four chicks were born alive, and of these, only one had a healthy birth and subsequent growth. The other three showed a transitory ataxia and one of them presented adult lumbar scoliosis and asymmetric pelvis.ConclusionsThe problem of recurrent spontaneous abortions is revisited in the light of these results. Some recent data suggest that soluble alloantigens may be candidates for a new etiological entity in recurrent spontaneous abortions. They can also be the cause of some congenital anomalies. The soluble, foreign, transitory antigens may have a similar effect although there is no supportive data in the literature.

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Antibodies to α-fetoprotein disrupt histogenesis in cultured chick retinae

Cited by 3 publications

References 15 publications

Sex steroid and tropic hormone levels may be associated with postoperative prognosis of vitrectomy in Korean postmenopausal women

Sex steroid and tropic hormone levels may be associated with postoperative prognosis of vitrectomy in Korean postmenopausal women

-Fetoprotein as a Biologic Response Modifier: Relevance to Domain and Subdomain Structure

Detection in chick embryo of fetoproteins not recognized by the dam's immune system and of soluble alloantigens. Presumptive teratogenic and abortogenic capacity of their specific IgY

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