Antibodies to listeriolysin O reflect the acquired resistance to Listeria monocytogenes in experimentally infected goats

Miettinen, Asko

doi:10.1016/0378-1097(91)90548-o

Cited by 10 publications

(12 citation statements)

References 2 publications

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“…Our feed prevalence data also suggest that while exposure to L. monocytogenes through contaminated feeds appears to be sufficient to cause listeriosis in small ruminants, clinical listeriosis in cattle may require additional contributing factors (since L. monocytogenes prevalence does not differ between bovine case and control farms). For example, the combination of exposure to particularly virulent L. monocytogenes subtypes and predisposing host factors, such as postpartum immune deficiency or pregnancy, may be required for clinical listeriosis in cattle (2,26).…”

Section: Discussionmentioning

confidence: 99%

Ecology and Transmission ofListeria monocytogenesInfecting Ruminants and in the Farm Environment

Nightingale¹,

Schukken

Nightingale

et al. 2004

Appl Environ Microbiol

349

316

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A case-control study involving 24 case farms with at least one recent case of listeriosis and 28 matched control farms with no listeriosis cases was conducted to probe the transmission and ecology of Listeria monocytogenes on farms. A total of 528 fecal, 516 feed, and 1,012 environmental soil and water samples were cultured for L. monocytogenes. While the overall prevalence of L. monocytogenes in cattle case farms (24.4%) was similar to that in control farms (20.2%), small-ruminant (goat and sheep) farms showed a significantly (P < 0.0001) higher prevalence in case farms (32.9%) than in control farms (5.9%). EcoRI ribotyping of clinical (n ‫؍‬ 17) and farm (n ‫؍‬ 414) isolates differentiated 51 ribotypes. L. monocytogenes ribotypes isolated from clinical cases and fecal samples were more frequent in environmental than in feed samples, indicating that infected animals may contribute to L. monocytogenes dispersal into the farm environment. Ribotype DUP-1038B was significantly (P < 0.05) associated with fecal samples compared with farm environment and animal feedstuff samples. Ribotype DUP-1045A was significantly (P < 0.05) associated with soil compared to feces and with control farms compared to case farms. Our data indicate that (i) the epidemiology and transmission of L. monocytogenes differ between small-ruminant and cattle farms; (ii) cattle contribute to amplification and dispersal of L. monocytogenes into the farm environment, (iii) the bovine farm ecosystem maintains a high prevalence of L. monocytogenes, including subtypes linked to human listeriosis cases and outbreaks, and (iv) L. monocytogenes subtypes may differ in their abilities to infect animals and to survive in farm environments.

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Section: Discussionmentioning

confidence: 99%

Ecology and Transmission ofListeria monocytogenesInfecting Ruminants and in the Farm Environment

Nightingale¹,

Schukken

Nightingale

et al. 2004

Appl Environ Microbiol

349

316

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“…Although antibodies are not the primary protective mechanism against listeriosis, seroconversion can be demonstrated after acute infection with L. monocytogenes, and antibodies may contribute to protection (16,17,42,43). Serum IgG and IgA were assessed before and after inoculation.…”

Section: Immune Responses (I) Elispot Studiesmentioning

confidence: 99%

“…A murine study showed that oral inoculation of L. monocytogenes expressing HIV-1-gag induced mucosal and systemic immunity to this viral antigen (45). After careful review of available data on oral inoculation of wild-type L. monocytogenes in primates (17) and farm animals, (34,42,43) clinical data available from case series (reviewed in reference 44), and reports of large outbreaks of listeriosis (2, 10, 47), we designed an initial safety study of rationally attenuated L. monocytogenes in adult volunteers.L. monocytogenes organisms are immunostimulatory and lack lipopolysaccharide, and molecularly defined attenuated mutants have been studied in animals.…”

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confidence: 99%

Safety and Shedding of an Attenuated Strain ofListeria monocytogeneswith a Deletion ofactA/plcBin Adult Volunteers: a Dose Escalation Study of Oral Inoculation

et al. 2002

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Listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. L. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in animal models. L. monocytogenes mutants lacking the ActA protein, which is essential for intracellular movement, are attenuated but retain immunogenicity in mice. Given the pathogenic potential of L. monocytogenes, we created an attenuated mutant strain bearing double deletions in the actA and plcB virulence genes for an initial clinical safety study of a prototype L. monocytogenes vector in adults. Twenty healthy volunteers received single escalating oral doses (10 6 to 10 9 CFU, 4 volunteers per dose cohort) of this attenuated L. monocytogenes, designated LH1169. Volunteers were monitored in the hospital for 14 days with frequent clinical checks and daily blood and stool cultures, and they were monitored for six additional weeks as outpatients. There were no positive blood cultures and no fevers attributable to the investigational inoculation. Most volunteers shed vaccine bacteria for 4 days or less, without diarrhea. One volunteer had a late positive stool culture during outpatient follow-up. Three volunteers had abnormal liver function test results temporally associated with inoculation; one could be reasonably attributed to another cause. In the highest-dose cohort, humoral, mucosal, and cellular immune responses to the investigational organism were detected in individual volunteers. Attenuated L. monocytogenes can be studied in adult volunteers without serious long-term health sequelae.Listeria monocytogenes is a gram-positive bacterium which has long been studied in mice to elucidate mechanisms of cellular immune responses to intracellular pathogens (29, 35). Schafer and colleagues initially proposed that escape of L. monocytogenes from the phagocytic vacuole into the eukaryotic cytoplasm might make these organisms efficient vectors for delivery of antigens to the major histocompatibility complex class I-restricted antigen processing pathways (48). L. monocytogenes vectors have been used to deliver lymphocytic choriomeningitis virus (LCMV) nucleoprotein antigens to mice, with subsequent protection against fatal challenge with LCMV (21, 52, 53). Recombinant L. monocytogenes expressing cottontail rabbit papillomavirus E1 antigen has been successfully used as a therapeutic immunogen in animals bearing papillomavirus-induced cutaneous tumors (28). L. monocytogenes is being pursued as a vector for antigens derived from human papillomaviruses (23) and human immunodeficiency virus type 1 (HIV-1) (40,41). A murine study showed that oral inoculation of L. monocytogenes expressing HIV-1-gag induced mucosal and systemic immunity to this viral antigen (45). After careful review of available data on oral inoculation of wild-type L. monocytogenes in primates (17) and farm animals, (34,42,43) clinical data avail...

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“…Several studies in human medicine have demonstrated the potential of this toxin as an antigen for the serological diagnosis of listeriosis in humans (6,18). Similar studies have shown that small ruminants develop detectable anti-listeriolysin O antibody titers during L. monocytogenes infections (2,24,25,26,29). Calves orally infected with L. monocytogenes (1,3,4,5) and dairy cows with intramammary L. monocytogenes infections (9, 10, 11) also produce significant humoral responses toward listeriolysin O.…”

mentioning

confidence: 93%

Use of Listeriolysin O and Internalin A in a Seroepidemiological Study of Listeriosis in Swiss Dairy Cows

Boerlin-Petzold

Jemmi

2003

J Clin Microbiol

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Recombinant listeriolysin O and internalin A were used as antigens in an enzyme-linked immunosorbent assay (ELISA) for the specific detection of anti-Listeria monocytogenes antibodies in cattle. The results showed sensitivities and specificities of 82 and 92%, respectively, for the listeriolysin O ELISA, and 100 and 90%, respectively, for the internalin A ELISA, respectively. The test may be useful for the confirmation of listeriarelated abortions and mastitis but does not seem to be indicated for use in the diagnosis of listeria-related encephalitis in cattle. A representative sample of 1,652 serum samples from the healthy dairy cattle population in Switzerland was tested by both ELISAs. The results showed that 11% of the healthy dairy cows in Switzerland simultaneously presented antibodies toward listeriolysin O and internalin A, and 48% of the farms had one or several animals simultaneously positive by assays with both antigens. Multivariable analysis at the farm level confirmed that feeding of silage represents a significant risk factor for a positive listeria serology. Detailed analysis identified corn silage but not grass silage as the major factor in this association. Cattle breed and hygiene on the farm were also identified as significant factors associated with the serological status of farms. In conclusion, the results of the study show that internalin A is a promising new antigen for use in listeria serology and that specific anti-L. monocytogenes antibodies are found in a significant proportion of healthy dairy cows in Switzerland.

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Antibodies to listeriolysin O reflect the acquired resistance to Listeria monocytogenes in experimentally infected goats

Cited by 10 publications

References 2 publications

Ecology and Transmission ofListeria monocytogenesInfecting Ruminants and in the Farm Environment

Ecology and Transmission ofListeria monocytogenesInfecting Ruminants and in the Farm Environment

Safety and Shedding of an Attenuated Strain ofListeria monocytogeneswith a Deletion ofactA/plcBin Adult Volunteers: a Dose Escalation Study of Oral Inoculation

Use of Listeriolysin O and Internalin A in a Seroepidemiological Study of Listeriosis in Swiss Dairy Cows

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