Use of Listeriolysin O and Internalin A in a Seroepidemiological Study of Listeriosis in Swiss Dairy Cows

Abstract

Recombinant listeriolysin O and internalin A were used as antigens in an enzyme-linked immunosorbent assay (ELISA) for the specific detection of anti-Listeria monocytogenes antibodies in cattle. The results showed sensitivities and specificities of 82 and 92%, respectively, for the listeriolysin O ELISA, and 100 and 90%, respectively, for the internalin A ELISA, respectively. The test may be useful for the confirmation of listeriarelated abortions and mastitis but does not seem to be indicated for use in the diagnosis of listeria-related encephalitis in cattle. A representative sample of 1,652 serum samples from the healthy dairy cattle population in Switzerland was tested by both ELISAs. The results showed that 11% of the healthy dairy cows in Switzerland simultaneously presented antibodies toward listeriolysin O and internalin A, and 48% of the farms had one or several animals simultaneously positive by assays with both antigens. Multivariable analysis at the farm level confirmed that feeding of silage represents a significant risk factor for a positive listeria serology. Detailed analysis identified corn silage but not grass silage as the major factor in this association. Cattle breed and hygiene on the farm were also identified as significant factors associated with the serological status of farms. In conclusion, the results of the study show that internalin A is a promising new antigen for use in listeria serology and that specific anti-L. monocytogenes antibodies are found in a significant proportion of healthy dairy cows in Switzerland.

Detection, identification, and typing of Listeria species from baled silages fed to dairy cows

“…in silage (29.5 vs. 6.2% for pH above or below 4.5, respectively). Similarly, several studies have highlighted that clinical listeriosis in ruminants is often associated with feeding poor-quality silage (Adams and Moss, 1995;Driehuis and Oude-Elferink, 2000;Boerlin et al, 2003). Therefore, spoilage prevention of feeds is an important point to prevent herd economic losses due to a spectrum of clinical conditions, including septicemia, meningitis, meningoencephalitis, abortion, and in some instances, death (Ramaswamy et al, 2007).…”

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

“…like somatic (O), flagellar (H), outer membrane protein (OMP) have been used for development of serological tests but resulted in poor sensitivity and specificity (Berche et al 1990;Chen & Chang 1996;Jadhav et al 2012). Therefore, virulent markers/protein antigen, such as LLO (Churchill et al 2006), internalins (InlA, InlB, InlC, InlC2, InlJ) (Boerlin et al 2003), actA protein (Jahangir et al 2012), phospholipases C Chaudhari, Malik, Bhanu, et al 2004) and autolysin p60 protein (Hess et al 1996) have been studied. Among these, LLO has been extensively studied and found suitable for development of serological tests like ELISA in humans (Berche et al 1990;Low & Donachie 1997;Barbuddhe et al 1999;Kaur et al 2006) and ELISA as well as immunoblotting in animals (Low & Donachie 1991;Miettinen & Husu 1991;Low et al 1992;Lhopital et al 1993;Barbuddhe et al 2000;Chaudhari et al 2001;Banu et al 2006).…”

Seropositivity of Field Veterinarians for Listeric Infection by Indirect ELISAs Employing Recombinant and Wild-Type Listeriolysin O

“…Therefore, it is important to have serodiagnostic tests based on (Berche et al,1990); internalins (InlA, InlB, InlC, InlC2, InlJ etc) (Das et al, 2013), the leucine rich repeat (LRR) proteins of Listeria spp. produced by virulence-linked family of genes (Boerlin et al, 2003); the actA gene encoded actin or Act A protein associated with cell-to-cell spread of the agent (Ellin Doyle, 2001), two phospholipases C namely the PI-PLC encoded by plcA gene and the PC-PLC encoded by plcB (Chaudhari et al, 2004a,b); the autolysin p60 protein (Hess et al, 1996).…”