Antibodies to human herpesvirus‐6 in Sjögren's syndrome

Baboonian, C.; Venables, P. J. W.; Maini, R. N.; Kangro, H. O.; Osman, H. K.

doi:10.1002/art.1780331122

Cited by 20 publications

(5 citation statements)

References 9 publications

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“…The lower prevalence found in our study reflect improvements made to the specificity of the IFA by inclusion of a saturation step [Ranger-Rogez et al, 19941 and use of F(ab'), fragments. Previous evaluations of the distribution of HHV-6 antibodies in PSS patients have given varied results [Biberfeld et al, 1988;Baboonian et al, 1990;Fillet et al, 19921 and are discussed elsewhere [Ranger-Rogez et al, 19941. When mean titers of antibody were measured in our study groups, PSS patients were found to have raised levels with respect to controls, thus probably reflecting polyclonal B-cell stimulation known to occur in this disease.…”

Section: Discussionmentioning

confidence: 98%

Large‐scale study suggests no direct link between human herpesvirus‐6 and primary Sjögren's syndrome

Vidal

Labrousse

Riché

et al. 1995

Journal of Medical Virology

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The aim of this study was to evaluate the role of human herpesvirus-6 (HHV-6) in the development of primary Sjögren's syndrome (PSS). Serum HHV-6 antibody levels, as measured by immunofluorescence assay (IFA) and the prevalence of HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) determined by polymerase chain reaction (PCR), were studied in 49 PSS patients and 50 control subjects, all in-patients in the University Hospital Internal Medicine ward, Limoges, France. In addition, portions of labial salivary gland were obtained from 34 patients and 15 controls, the presence of viral DNA being detected by the same PCR technique. The results were then compared with clinical observations of systemic disease manifestations in patients and a histological study of salivary gland involvement. No significant difference in HHV-6 seroprevalence was found between control subjects (50.0%) and patients (63.3%) nor was there any statistically significant difference between patient and control groups for total viral DNA in PBMCs (22.4%, 12.0%) and salivary glands (8.8%, 6.6%). Analysis of clinical and histological data revealed no detectable correlation between disease severity and viral involvement. Tests for HHV-6A and HHV-6B proved positive in patient and control groups, HHV-6B being the most frequently encountered type in both groups. In conclusion, the results of this large-scale trial does not confirm the suspected direct role of HHV-6 in the etiology of PSS.

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Section: Discussionmentioning

confidence: 98%

Large‐scale study suggests no direct link between human herpesvirus‐6 and primary Sjögren's syndrome

Vidal

Labrousse

Riché

et al. 1995

Journal of Medical Virology

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“…In another study, 49 patients with primary Sjögren's syndrome and 50 age-and sex-matched normal controls had similar rates of HHV-6 seroprevalence or prevalence of HHV-6 DNA detected by PCR in PBMCs or salivary gland biopsy specimens (424). HHV-6 antibody titers in 25 women with rheumatoid arthritis were either lower or slightly higher than in an equal number of age-and sexmatched healthy controls, when measured by IFA or enzymelinked immunosorbent assay, respectively (38). HHV-6 has been isolated from peripheral blood lymphocytes of several patients with various collagen vascular diseases, most often from patients with systemic lupus erythematosus (295).…”

Section: Collagen Vascular Diseasesmentioning

confidence: 89%

“…The relevant studies are summarized here. A cohort of 25 women with primary Sjögren's syndrome had no significant differences in HHV-6 seroprevalence or antibody titers compared with a group of 25 age-matched healthy women (38). In another study, 49 patients with primary Sjögren's syndrome and 50 age-and sex-matched normal controls had similar rates of HHV-6 seroprevalence or prevalence of HHV-6 DNA detected by PCR in PBMCs or salivary gland biopsy specimens (424).…”

Section: Collagen Vascular Diseasesmentioning

confidence: 94%

Human herpesvirus 6.

Braun

Dominguez

Pellett

1997

Clinical Microbiology Reviews

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“…The epidemiology and clinical consequences of HHV-6A infections are not as fully defined; however, primary HHV-6A infection has been reported in one case of exanthem subitum (25). HHV-6 infections have also been associated with encephalitis (4), hepatitis (3), multiple sclerosis, chronic fatigue syndrome, and some lymphomas, although a formal causal association has not been proven (5,7,31,48). In individuals who are immunosuppressed by drugs, such as transplant patients, or individuals infected with human immunodeficiency virus, HHV-6 is emerging as an important pathogen.…”

mentioning

confidence: 99%

The U69 Gene of Human Herpesvirus 6 Encodes a Protein Kinase Which Can Confer Ganciclovir Sensitivity to Baculoviruses

Ansari

Emery

1999

J Virol

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The protein encoded by the U69 open reading frame (ORF) of human herpesvirus 6 (HHV-6) has been predicted to be a protein kinase. To investigate its functional properties, we have expressed the U69 ORFs from both HHV-6 variants, A and B, by using recombinant baculoviruses (BV6AU69 and BV6BU69). Nickel agarose and antibody affinity chromatography was used to purify the proteins to homogeneity and when incubated with [γ-32P]ATP, both U69 proteins became phosphorylated on predominantly serine residues. These data strongly suggest that U69 is a protein kinase which autophosphorylates. The phosphorylation reaction was optimal at physiological pH and low NaCl concentrations. It required the presence of Mg2+ or Mn2+, and Mg2+ was able to support phosphorylation over a wider range of concentrations than Mn2+. Both ATP and GTP could donate phosphate in the protein kinase assay and the former was more efficient. U69 was capable of phosphorylating histone and casein (serine/threonine kinase substrates) but not enolase (a tyrosine kinase substrate). For the autophosphorylation reaction, the Michaelis constants for ATP of baculovirus-expressed HHV-6A and HHV-6B U69 were calculated to be 44 and 11 μM, respectively. U69 is a homologue of the UL97 gene encoded by human cytomegalovirus which has been shown to phosphorylate the antiviral drug ganciclovir (GCV). We analyzed whether the U69 ORF alone was capable of conferring GCV sensitivity on baculoviruses BV6AU69 and BV6BU69. In plaque reduction experiments, these baculoviruses displayed a GCV-sensitive phenotype compared to a control baculovirus (BVLacZ). The 50% inhibitory concentrations (IC50) of BV6AU69 and BV6BU69 were calculated to be 0.35 and 0.26 mM, respectively, whereas the control baculovirus had an IC50 of >1.4 mM. This shows that the U69 gene product is the only one required to confer GCV sensitivity on baculovirus.

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Antibodies to human herpesvirus‐6 in Sjögren's syndrome

Cited by 20 publications

References 9 publications

Large‐scale study suggests no direct link between human herpesvirus‐6 and primary Sjögren's syndrome

Large‐scale study suggests no direct link between human herpesvirus‐6 and primary Sjögren's syndrome

Human herpesvirus 6.

The U69 Gene of Human Herpesvirus 6 Encodes a Protein Kinase Which Can Confer Ganciclovir Sensitivity to Baculoviruses

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