Antibodies Isolated from Rheumatoid Arthritis Patients against Lysine-Containing Proteus mirabilis O3 (S1959) Lipopolysaccharide May React with Collagen Type I

Abstract

Most rheumatic diseases, including rheumatoid arthritis (RA), are characterized by immune disorders that affect antibody activity. In the present study, using Dot blot and ELISA assay, we showed that patients with rheumatic disease produced significantly more antibodies against lipopolysaccharide (LPS) P. mirabilis O3 compared to healthy donors (p < 0.05), and affinity purified antibodies against LPS O3 may cross-react with collagen type I. It was demonstrated that purified of antibodies isolated from RA patients sera, reacted stronger with the collagen than healthy donors (p = 0.015), and cross-reaction was correlated with level of anti-citrullinated peptide antibodies (r = 0.7, p = 0.003). Moreover, using six different lipopolysaccharides were demonstrated the significant correlations in sera reactivity among lysine-containing lipopolysaccharides observed in patients’ sera (p < 0.05). Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) it was shown that unique wavenumbers of sera spectra correlate with reactivity with lipopolysaccharides allowing distinguish patients from healthy blood donors. Antibodies adsorption by synthetic antigens shows that in patients’ group anti-LPS O3 antibodies can be adsorbed by both amides of galacturonic acid and lysine or threonine, which suggests less specificity of antibodies binding with non-carbohydrate LPS component. The observed correlations suggest that non-carbohydrate components of LPS may be an important epitope for less specific anti-LPS antibodies, which might lead to cross-reactions and affect disease development.

Mining the capacity of human-associated microorganisms to trigger rheumatoid arthritis—A systematic immunoinformatics analysis of T cell epitopes

“…In contrast to this, a large fraction of proposed RA-triggering candidates refers to the microbes previously uncharacterized as contributing factors to develop this autoimmune condition. However, major players, which have been identified in the literature as the most probable RA-triggers, such as P. mirabilis [33][34][35][36][37][38][39] and M. tuberculosis [40][41][42][43][44][45], also found their place in the proposed lists of candidate species (Table 1), corroborating the applied search methodology. Additionally, a set of predicted T cell epitopes, with significant sequence…”

Haemophilus parasuis (Glaesserella parasuis) as a Potential Driver of Molecular Mimicry and Inflammation in Rheumatoid Arthritis

“…Several candidates have been proposed as disease-promoting microbes in RA, including viruses (44)(45)(46)(47)(48), mycobacteria (49)(50)(51), and other bacteria (14,(52)(53)(54)(55)(56). Thus far, however, none have been found as a candidate that could satisfy the criteria to be the culprit of RA.…”

Proteomics Landscape Mapping of Organ‐Resolved Behçet's Disease Using In‐Depth Plasma Proteomics for Identifying Hyaluronic Binding Protein 2 Expression Associated With Vascular Involvement

“…Furthermore, we identified the proteomics characteristics of the patients with BD with different organ involvement. Our bioinformatics analyses effectively illustrate the functions of the protein features of each organ phenotype, as exemplified by the association between autoimmunity and the arthritis phenotype with symptoms of arthralgia (29)(30)(31). Major vascular events, ocular lesions, and neurologic involvement are the major causes of morbidity and mortality (16).…”