Antibodies Against Mimotopes of Simian Virus 40 Large T Antigen, the Oncoprotein, in Serum Samples From Elderly Healthy Subjects

Mazzoni, Elisa; Guerra, Giovanni; Casali, Maria Vittoria; Pietrobon, Silvia; Bononi, Ilaria; Puozzo, Andrea; Tagliapietra, Andrea; Nocini, Pier Francesco; Tognon, Mauro; Martini, Fernanda

doi:10.1002/jcp.25405

Cited by 8 publications

(17 citation statements)

References 45 publications

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“…Preliminary ELISA results indicated that the two selected peptides did not cross-react with BKPyV and JCPyV hyperimmune sera employed as controls. The two A and D peptides map on the LT oncoprotein, 2 as shown earlier (24, 25). The a.a. sequences of the two LT A and D peptides are as follows:

LT A: NH2-G S F Q A P Q S S Q S V H D H N Q P Y H I-COOH (Tag a.a. 669–689).

LT D: NH2-H E T G I D S Q S Q G S F Q A P Q S S Q S V H D-COOH (Tag a.a. 659–682).

…”

Section: Methodsmentioning

confidence: 54%

“…Different wells were covered with 100 µl containing the following sera: a positive control represented by the immune rabbit serum containing anti-SV40 LT Ab; the negative controls were immune sera with anti-BKPyV and anti-JCPyV Ab and three human serum samples tested SV40-negative in earlier studies (24, 25). Human serum samples from pregnant women and non-pregnant women were diluted in Low Cross-Buffer pH 7.2 (Candor Bioscience, Wangen, Germany) at 1:20 and were added to the plate.…”

Section: Methodsmentioning

confidence: 99%

“…The three SV40-negative control sera were selected from those below the cutoff value determined with second-degree polynomial regression by plotting the ranked net OD individual values for each peptide. The OD representation was obtained from a second-degree polynomial regression for LT A and D mimotopes, as reported before for BKPyV and MCPyV VLPs (24, 25). Our representations show an inflection point for peptide A and peptide D at 0.19–0.18, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Novel indirect ELISAs with specific SV40 mimotopes, as synthetic peptides, representing viral capsid proteins VP 1–3 (23) and viral LT oncoprotein (24, 25) seems to circumvent these problems, i.e., the cross-reactivity among closely related polyomaviruses. Recent studies with these novel ELISAs documented SV40 Ab in healthy subjects with an estimated seroprevalence of 18–20%.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular biology and immunological investigations reported on the presence of SV40 footprints in samples from healthy subjects and patients who had not administered with SV40-contaminated vaccines (24, 26). These studies indicate that the human-to-human contagion could be responsible of the SV40 infection in the human population.…”

Section: Introductionmentioning

confidence: 99%

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Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

et al. 2017

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Simian virus 40 (SV40) large T antigen (LT) coding sequences were revealed in different human samples, whereas SV40 antibodies (Ab) were detected in human sera of cancer patients and healthy individuals, although with a lower prevalence. Previous studies carried out by the neutralization assay gave a SV40 seroprevalence, in the general population, up to 8%, although higher rates, 12%, were detected in kidney transplant children, in a group of HIV-positive patients, and in healthy females. In this study, serum samples from pregnant women, together with those from non-pregnant women, were analyzed to check the prevalence of IgG Ab reacting to SV40 LT antigens. Serum samples were collected from pregnant and non-pregnant women, with the same mean age. Women were in the range of 15–48 years old. Samples were assayed by an indirect ELISA employing specific SV40 LT mimotopes as antigens, whereas functional analysis was performed by neutralization of the viral infectivity in cell cultures. As a control, sera were analyzed for Ab against BK polyomavirus (BKPyV), which is a human polyomavirus homologous to SV40. Statistical analyses employed chi-square with Yates’ correction, and Student’s t tests. Indirect ELISAs indicated that pregnant women tested SV40 LT-positive with a prevalence of 17% (23/134), whereas non-pregnant women had a prevalence of 20% (36/180) (P > 0.05). Ab against BKPyV were detected with a prevalence of 80% in pregnant women and with a prevalence of 78% in non-pregnant women. These data indicate that SV40 infects at a low prevalence pregnant women. We may speculate that SV40, or a close human polyomavirus still undetected, could be transmitted from mother to fetus.

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LT A: NH2-G S F Q A P Q S S Q S V H D H N Q P Y H I-COOH (Tag a.a. 669–689).

LT D: NH2-H E T G I D S Q S Q G S F Q A P Q S S Q S V H D-COOH (Tag a.a. 659–682).

…”

Section: Methodsmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

et al. 2017

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Antibodies reacting to mimotopes of Simian virus 40 large T antigen, the viral oncoprotein, in sera from children

Mazzoni

Frontini

Rotondo

et al. 2018

Journal Cellular Physiology

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Recent data indicate that the Simian virus 40 (SV40) infection appears to be transmitted in humans independently from early SV40-contaminated antipolio vaccines. Serum antibodies against SV40 large T antigen (Tag) were analyzed in children/adolescents and young adults. To investigate antibodies reacting to SV40 Tag antigens, serum samples (n = 812) from children and young adults were analyzed by indirect ELISAs using specific SV40 Tag mimotopes. Mimotopes were synthetic peptides corresponding to SV40 Tag epitopes. In sera (n = 412) from healthy children up to 17 years old, IgG antibodies against SV40 Tag mimotopes reached an overall prevalence of 15%. IgM antibodies against SV40 Tag were detected in sera of children 6-8 months old confirming and extending the knowledge that SV40 seroconversion occurs early in life. In children/adolescents affected by different diseases (n = 180) SV40 Tag had a prevalence of 18%, being the difference no significant compared to healthy subjects (n = 220; 16%) of the same age. Our immunological data indicate that SV40 circulates in children and young adults, both in healthy conditions and affected by distinct diseases. The IgM detection in sera from healthy children suggests that the SV40 infection/seroconversion occurs early in life (>6 months). Our immunological data support the hypothesis that SV40, or a closely related still unknown polyomavirus, infects humans. The SV40 seroprevalence is lower than common polyomaviruses, such as BKPyV and JCPyV, and other new human polyomaviruses. In addition, our immunological surveillance indicates a lack of association between different diseases, considered herein, and SV40.

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High prevalence of antibodies reacting to mimotopes of Simian virus 40 large T antigen, the oncoprotein, in serum samples of patients affected by non-Hodgkin lymphoma

Mazzoni

Pietrobon

Bilancia

et al. 2017

Cancer Immunol Immunother

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A new immunological investigation was carried out to study the association between non-Hodgkin lymphoma and Simian virus 40 (SV40). To this end, a new indirect ELISA was employed with two mimotopes from SV40 large T antigen (Tag), the viral oncoprotein, to analyse for specific reactions to antibodies in sera from non-Hodgkin lymphoma patients and controls, represented by healthy subjects (HS) and breast carcinoma (BC) patients. This study allowed us to assay a new sera collection from non-Hodgkin lymphoma patients (NHL, n = 254). To verify the association between NHL and SV40 Tag, two totally independent cohorts were analysed: NHL1 n = 150 and NHL2 n = 104. The epidemiological survey included sera from HS1, n = 150; HS2, n = 104 and BC, n = 78. This new indirect ELISA revealed that antibodies against SV40 Tag mimotopes are detectable in NHL1 and NHL2 sera with a prevalence of 37 and 36%, respectively. The prevalence of SV40-antibodies detected in both NHL1 and NHL2 cohorts differs statistically from controls, at 19% for HS1 (p < 0.01), HS2 (p < 0.05) and BC patients (p < 0.05). This study, carried out with an immunological assay with specific Tag oncoprotein mimotopes of Simian virus 40, reports the presence of IgG antibodies against the large Tumour antigen in non-Hodgkin lymphomas for the first time. Our immunological data with two independent NHL cohorts show a statistically significant association between Simian virus 40 Tag and non-Hodgkin lymphoma. These results suggest that SV40-positive non-Hodgkin lymphomas could be treated differently from those tested SV40-negative.

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Antibodies Against Mimotopes of Simian Virus 40 Large T Antigen, the Oncoprotein, in Serum Samples From Elderly Healthy Subjects

Cited by 8 publications

References 45 publications

Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

Antibodies reacting to mimotopes of Simian virus 40 large T antigen, the viral oncoprotein, in sera from children

High prevalence of antibodies reacting to mimotopes of Simian virus 40 large T antigen, the oncoprotein, in serum samples of patients affected by non-Hodgkin lymphoma

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