Antibiotics susceptibility of quinolones against Salmonella spp. strains isolated and molecularly sequenced for gyrA gene

Macías-Farrera, Guadalupe Patricia; Jiménez, Roberto Montes de Oca; Guerrero, Jorge Antonio Varela; Tenorio-Borroto, Esvieta; Rivera-Ramírez, Fabiola; Monroy-Salazar, Humberto Gustavo; Yong-Ángel, Gilberto

doi:10.1016/j.micpath.2017.11.067

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Single point mutations in the quinolone resistance-determining region of gyrA reduce the sensitivity of fluoroquinolones (e.g., ciprofloxacin). However, antimicrobial resistance may require two or more mutations in the quinolone-determining region of gyrA, gyrB, parC, and parE [46][47][48]. Plasmids, transferred between different bacterial species and clones, can carry antimicrobial resistance or virulence genes.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Genomic Characterization of Salmonella Isolated from Pigeons in China

Zhang,

Lu,

Zhao

et al. 2024

Transboundary and Emerging Diseases

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Salmonellosis is one of the important bacterial infectious diseases affecting the health of pigeons. Heretofore, the epidemiological characteristics of Salmonella in pigeon populations in China remain largely unclear. The present study investigated the antimicrobial resistance and genomic characteristics of Salmonella isolates in pigeons in different regions of China from 2022 to 2023. Thirty-two Salmonella isolates were collected and subjected to 24 different antimicrobial agents, representing nine categories. The results showed that these isolates were highly resistant to cefazolin (100%), gentamicin (100%), tobramycin (100%), and amikacin (100%). Three or more types of antimicrobial resistance were present in 90.62% of the isolates, indicating multidrug resistance. Furthermore, using whole genome sequencing technology, we analyzed the profiles of serotypes, multilocus sequence typing, virulence genes, antimicrobial resistance genes, and plasmid replicons and constructed phylogenetic genomics to determine the epidemiological correlation among these isolates. All strains belonged to Salmonella Typhimurium var. Copenhagen and exhibited five antimicrobial resistance genes and more than 150 Salmonella virulence genes. Moreover, each isolate contained both the IncFIB(S) and IncFII(S) plasmids. In addition, phylogenetic analysis showed that all isolates were very close to each other, and isolates from the same region clustered in the same branch. Overall, our findings provide the first evidence for the epidemiological characteristics of Salmonella in pigeons of China, highlighting the importance of preventing salmonellosis in pigeons.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Genomic Characterization of Salmonella Isolated from Pigeons in China

Zhang,

Lu,

Zhao

et al. 2024

Transboundary and Emerging Diseases

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“…A single substitution (T57S) was revealed in parC , whereas two different substitutions (S83E/Y and D124G/E) were reported in gyrA . In contrast, QRDR substitutions in gyrA of Salmonella isolates were reported to be S83F/Y and D87N in Mexico [31], S83F/I/T, D87N, H80N, and N78H in China [26], S83F and D87Y in Japan [32], and S83F and D87N in the USA [29]. T57S has been previously reported in isolates from Thailand, China, Korea, and Poland [22, 27, 33–35].…”

Section: Discussionmentioning

confidence: 99%

Fluoroquinolone resistance in non-typhoidal Salmonella enterica isolated from slaughtered pigs in Thailand

Poomchuchit

Kerdsin

Chopjitt

et al. 2021

Journal of Medical Microbiology

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Introduction. The emergence and spread of non-typhoidal Salmonella enterica (NTS) serovars resistant to fluoroquinolones and third- and higher-generation cephalosporins is a matter of great concern. Antimicrobial-resistant NTS is increasingly being discovered in humans, animals, food animals, food products, and agricultural environments. Pigs are considered a major reservoir of antimicrobial-resistant Salmonella spp. Hypothesis/Gap Statement. Fluoroquinolone-resistant Salmonella spp. warrant further surveillance and characterization for a better understanding of the bacteria isolated from animals. Aim. NTS isolated from pork from slaughterhouses across Thailand were characterized in terms of their serovars; resistance to fluoroquinolones, third-generation cephalosporins, and carbapenems; and antimicrobial resistance genes. Methodology. A total of 387 NTS isolates, collected from slaughtered pigs in ten provinces across Thailand between 2014 and 2015, were characterized based on their serovars, antimicrobial resistance genes, and susceptibility to fluoroquinolones, third-generation cephalosporins, and carbapenems. Results. Among all NTS isolates, S. enterica serovar Rissen was predominant. Antimicrobial resistance was exhibited in 93/387 isolates (24 %). Although 24 (6.2 %) isolates were susceptible to all the tested antimicrobials, they were found to possess β-lactamase genes, such as bla TEM, bla SHV, or bla CTX-M. Mobilized colistin-resistant genes (mcr) and resistance to colistin were not observed in any tested isolate. Carbapenem resistance was detected in ten isolates (10.7 %); however, bla KPC, bla NDM, bla OXA-48-like, and bla IMP were not present. Among the 93 antimicrobial-resistant isolates, 87.1 % showed fluoroquinolone resistance with the quinolone resistance gene (qnrS) combined with topoisomerase genes parC (T57S) or gyrA (S83E/Y and D124E/G) substitutions, or topoisomerase gene substitutions alone. Conclusion. We found high fluoroquinolone resistance rates among the NTS isolates from pigs from slaughterhouses. The fluoroquinolone resistance mechanism in NTS was associated with the combination of qnrS and substitutions in gyrA, parC, or both. To prevent the transmission of antimicrobial-resistant NTS between animals and humans, continuous monitoring, surveillance, and regulation of Salmonella in the pork supply chain are pivotal.

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“…Las cepas de Salmonella pueden ganar resistencia a las quinolonas a través de una disminución de la expresión de las porinas y/o una mayor expresión de sistemas de expulsión no específicos, sistemas de expulsión energético-dependientes, tales como las bombas AcrAB-TolC. Sin embargo, estos mecanismos producen sólo un bajo nivel de resistencia a las quinolonas y son clínicamente importantes únicamente cuando concurren con alteraciones en las enzimas diana como la DNA girasa (Macías-Farrera et al, 2018).…”

Section: Resistencia a Las Quinolonas Y Fluoroquinolonasunclassified

Caracterización fenotípica y genotípica de la resistencia a antibióticos en aislamientos de "Salmonella enterica" de carne de ave: efecto de dosis subinhibitorias de biocidas sobre los patrones y los mecanismos de resistencia = Phenotypic and genotypic characterization of antibiotic resistance in "Salmonella enterica" isolates from poultry: effect of subinhibitory doses of biocides on patterns and mechanisms of resistance

Gonzalez¹

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Materiales y métodos……………………………………………………………….100 2.1 Cepas………………………………………………………………………..100 2.2 Pruebas de susceptibilidad a antibióticos………………………………..101 2.3 Caracterización genotípica………………………………………………..102 3 Resultados y Discusión…………………………………………….………………105 3.1 Resistencia a antibióticos………………………………………………….105 3.2 Genes de resistencia a los antibióticos y factores de virulencia………………………………………………………………………………..113

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Antibiotics susceptibility of quinolones against Salmonella spp. strains isolated and molecularly sequenced for gyrA gene

Cited by 4 publications

References 30 publications

Antimicrobial Resistance and Genomic Characterization of Salmonella Isolated from Pigeons in China

Antimicrobial Resistance and Genomic Characterization of Salmonella Isolated from Pigeons in China

Fluoroquinolone resistance in non-typhoidal Salmonella enterica isolated from slaughtered pigs in Thailand

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