Fluoroquinolone resistance in non-typhoidal Salmonella enterica isolated from slaughtered pigs in Thailand

Poomchuchit, Suleepon; Kerdsin, Anusak; Chopjitt, Peechanika; Boueroy, Parichart; Hatrongjit, Rujirat; Akeda, Yukihiro; Tomono, Kazunori; Nuanualsuwan, Suphachai; Hamada, Shigeyuki

doi:10.1099/jmm.0.001386

Cited by 5 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This clearly illustrates that the presence of mutations does not necessarily correspond to phenotypic resistance ( Sáenz et al, 2003 ). On the other hand, one study in nontyphoidal S. enterica isolated from pigs in Thailand showed resistance to fluoroquinolones, either in the presence or absence of genes and/or mutations, and par C mutation (T57) was found in 62.4% of the isolates ( Poomchuchit et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and genomic characterization of Salmonella enterica serovar Senftenberg isolates in production animals from the United States

Srednik

Morningstar‐Shaw²,

Hicks³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

In the USA, Salmonella enterica subspecies enterica serovar Senftenberg is among the top five serovars isolated from food and the top 11 serovars isolated from clinically ill animals. Human infections are associated with exposure to farm environments or contaminated food. The objective of this study was to characterize S. Senftenberg isolates from production animals by analyzing phenotypic antimicrobial resistance profiles, genomic features and phylogeny.Salmonella Senftenberg isolates (n = 94) from 20 US states were selected from NVSL submissions (2014–2017), tested against 14 antimicrobial drugs, and resistance phenotypes determined. Resistance genotypes were determined using whole genome sequencing analysis with AMRFinder and the NCBI and ResFinder databases with ABRicate. Plasmids were detected using PlasmidFinder. Integrons were detected using IntFinder and manual alignment with reference genes. Multilocus-sequence-typing (MLST) was determined using ABRicate with PubMLST database, and phylogeny was determined using vSNP.Among 94 isolates, 60.6% were resistant to at least one antimicrobial and 39.4% showed multidrug resistance. The most prevalent resistance findings were for streptomycin (44.7%), tetracycline (42.6%), ampicillin (36.2%) and sulfisoxazole (32.9%). The most commonly found antimicrobial resistance genes were aac(6′)-Iaa (100%), aph(3″)-Ib and aph(6)-Id (29.8%) for aminoglycosides, followed by blaTEM-1 (26.6%) for penicillins, sul1 (25.5%) and sul2 (23.4%) for sulfonamides and tetA (23.4%) for tetracyclines. Quinolone-resistant isolates presented mutations in gyrA and/or parC genes. Class 1 integrons were found in 37 isolates. Thirty-six plasmid types were identified among 77.7% of the isolates. Phylogenetic analysis identified two distinct lineages of S. Senftenberg that correlated with the MLST results. Isolates were classified into two distinct sequence types (ST): ST14 (97.9%) and ST 185 (2.1%). The diversity of this serotype suggests multiple introductions into animal populations from outside sources.This study provided antimicrobial susceptibility and genomic characteristics of S. Senftenberg clinical isolates from production animals in the USA during 2014 to 2017. This study will serve as a base for future studies focused on the phenotypic and molecular antimicrobial characterization of S. Senftenberg isolates in animals. Monitoring of antimicrobial resistance to detect emergence of multidrug-resistant strains is critical.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and genomic characterization of Salmonella enterica serovar Senftenberg isolates in production animals from the United States

Srednik

Morningstar‐Shaw²,

Hicks³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Moreover, other studies in Cambodia have found Salmonella in chicken meat (Nadimpalli et al, 2019) and vegetables that are in contact with meat during the selling period at the market (Schwan et al, 2021). In the current study, the prevalence of Salmonella in meat was relatively high compared to studies in nearby countries, including studies from Vietnam and Thailand Poomchuchit et al, 2021). Pathogenic Salmonella enterica have also been isolated from multiple sources, including humans, animals, and food in Cambodia (van Cuyck et al, 2011;Schwan et al, 2021).…”

Section: Growth Model At Householdmentioning

confidence: 39%

Quantitative risk assessment of salmonellosis in Cambodian consumers through chicken and pork salad consumption

Rortana¹,

Dang-Xuan²,

Nguyen-Viet³

et al. 2022

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

Salmonella is a globally important foodborne bacterial pathogen that poses a high risk to human health. This study aimed to estimate the risk to Cambodian consumers from acquiring salmonellosis after consuming chicken and pork salad, using a quantitative microbial risk assessment (QMRA). Chicken and pork salads are typical Cambodian dishes containing raw vegetables and boiled chicken meat or pork. As previously described, chicken meat and pork samples (n = 204 of each) were collected from traditional markets in 25 Cambodian provinces to generate data on Salmonella contamination. Salad preparation and consumption practices were surveyed in 93 Cambodian households and this information was used to design an experiment to assess Salmonella cross-contamination from raw meat to ready-to-eat salad. In the part of the study reported here, data on consumption, Salmonella in salad, dose-response, and predicted salmonellosis were modeled using Monte Carlo simulations at 10,000 iterations. The prevalence of Salmonella in chicken meat and pork were set to 42.6 and 45.1%, respectively, with average most probable number (MPN) per gram of Salmonella in chicken meat was 10.6 and in pork 11.1 MPN/g, based on an earlier study. Half of the interviewed households cooked meat for the salad directly after purchase. The QMRA model showed that the modeled annual risk of salmonellosis from consuming chicken salad, pork salad and both chicken and pork salad were 11.1% probability of illness per person per year (90% CI 0.0–35.1), 4.0% (90% CI 0.0–21.3), and 14.5% (90% CI 0.0–33.5), respectively. The factors most influencing the estimate were cross-contamination while preparing the salad, followed by the prevalence of Salmonella in chicken meat and pork at the market. The wide confidence interval for the incidence was mainly due to the variability in reducing bacteria concentration by cooking and salad consumption. The predicted risk of salmonellosis due to chicken and pork salad consumption is high, and the study provides evidence supporting control measures of improving the safety of retailed chicken and pork obtained from markets to households and improving food preparation methods in the household.

show abstract

“…Moreover, other studies in Cambodia have found Salmonella in chicken meat and vegetables that are in contact with meat during the selling period at the market . In the current study, the prevalence of Salmonella in meat was relatively high compared to studies in nearby countries, including studies from Vietnam and Thailand Poomchuchit et al, 2021). Pathogenic Salmonella enterica have also been isolated from multiple sources, including humans, animals, and food in Cambodia .…”

Section: Discussionmentioning

confidence: 52%

Foodborne bacteria in the Cambodian meat value chain: Emphasis on the risk of Salmonella in chicken and pork from traditional markets to household consumption

Rortana¹

2023

Acta Universitatis Agriculturae Sueciae

View full text Add to dashboard Cite

Foodborne pathogens, such as Salmonella and Staphylococcus aureus, pose a high risk to human health globally. Using quantitative microbial risk assessment (QMRA), this thesis estimated the risk of salmonellosis to Cambodian consumers eating contaminated chicken and pork salad. Chicken meat and pork samples (n=204 each) were collected from traditional markets in 25 provinces in Cambodia for analyses of Salmonella and also S. aureus. Practices used for preparing chicken and pork salads in 93 Cambodian households were surveyed and used to design an experiment assessing Salmonella cross-contamination from raw meat to ready-to-eat salad in four scenarios. Salmonella prevalence in chicken meat and pork was found at 42.6% (87/204) and 45.1% (92/204), respectively, with mean Salmonella concentration of 10.6 MPN/g in chicken meat and 11.1 MPN/g in pork. Salmonella contamination, salad consumption and dose-response were modelled using Monte Carlo simulations with 10,000 iterations. The QMRA model showed that the annual estimated risk of salmonellosis from consuming chicken salad, pork salad and both salads was 11.2% (90% CI 0.0-35.1), 4.0% (0.0-21.3) and 14.5% (0.0-33.5), respectively. Thus one in 10 chicken salad consumers in Cambodia was at risk of contracting salmonellosis annually. The factor with the most decisive influence on risk estimates was crosscontamination while preparing salad, followed by high prevalence of Salmonella on chicken meat/pork at the market. These results indicate a need to implement control measures, including monitoring the safety of retail chicken and pork at markets and improving hygiene in practices and equipment used during salad preparation in households.

show abstract

Fluoroquinolone resistance in non-typhoidal Salmonella enterica isolated from slaughtered pigs in Thailand

Cited by 5 publications

References 39 publications

Antimicrobial resistance and genomic characterization of Salmonella enterica serovar Senftenberg isolates in production animals from the United States

Antimicrobial resistance and genomic characterization of Salmonella enterica serovar Senftenberg isolates in production animals from the United States

Quantitative risk assessment of salmonellosis in Cambodian consumers through chicken and pork salad consumption

Foodborne bacteria in the Cambodian meat value chain: Emphasis on the risk of Salmonella in chicken and pork from traditional markets to household consumption

Contact Info

Product

Resources

About