Antibiotics sensitivity of Candida clinical and food-borne isolates.

Abstract

Candida is a yeast species recognized as the most frequent etiological agent of systemic and invasive thrush in humans. Invasions can affect all tissues, organs and systems of human in various stages of development. In the last 10 years Candida infections have increased 15 times. The purpose of our study was to determine the sensitivity of four antibiotics belonging to three different groups of antifungal agents against clinical and food-borne Candida strains. Our studies showed that of all tested strains, 7% was resistant to nystatin, 32% to fluconazole, 23% to voriconazole, and no strains grew in the presence of caspofungin. Despite the differences in biochemical profiles of clinical and food-borne isolates of Candida, a group of strains showing resistance to antibiotics include both types of isolates. At the same time circulating of antibiotic-resistant strains outside the hospital environment and the yeast infection via food is possible.

Disk diffusion Method in Enriched Mueller Hinton agar for determining susceptibility of Candida isolates from various clinical specimens

“…22 In different countries resistant pattern against fluconazole varied among studies. Quindos et al (1999), 23 Maroszyñska et al (2013) 18 and Salehei et al…”

“…Unfortunately it is shown that resistance to antifungal azoles has increased. 18,24 The increase in resistance to fluconazole is of serious health concern as it is the most commonly used azole for superficial as well as deep candidiasis. The results of present study are in accordance with the results of other studies in the respect of amphotericin B and fluconazole susceptibility.…”

“…8 The response to the other antifungal agents, for which there is no standardized method and no interpretive breakpoints, were interpreted according to the manufacturers' instructions (Mast group limited, UK) and were adopted from published studies ( Table-1). 9,17,18 Flooding procedure Flooded plates were prepared according to Barry et al 15 A stock solution of methylene blue (5mg/ ml) was prepared and refrigerated at 2 to 8°C. In 100 ml of 40% glucose solution, 200µl of the stock methylene blue was added to give 10µg of methylene blue per ml of 40% glucose (GMB solution).…”

Antimicrobial Activity of Some Essential Oils on Candida Genus Clinical Isolates

“…: to identify microscopic fungi -on Sabouraund Dextrose Agar (Himedia) medium; to identify streptococci and Neisseria -blood agar, Enterobacteriaceae − Endo agar (Farmaktiv, Ukraine). We identified the yeasts based on macromorphology, micromorphology, and physiological and biochemical tests [7].…”

“…To determine the antimicrobial activity of essential oils as test cultures were used the typical strain of yeast from the American Type Culture Collection, USA Candida albicans ATCC 885-653; 19 clinical strains Candida isolated from the sputum of people with tuberculosis, which taken antibiotic therapy during long period. Antimicrobial activity was determined using disk diffusion method, using 6 mm sterilized filter paper discs [7,8]. Cultures of Candida were previously grown on the elective nutrient media Sabourand Dextrose Agar (SDA) 30 °C 48 h.…”