Antibiotics can cause weight loss by impairing gut microbiota in mice and the potent benefits of lactobacilli

Abstract

This study assessed whether antibiotics could alter gut microbiota to affect host growth and the possibility of alleviation by lactobacilli. We divided four-week-old BABL/c mice into control (Ctrl), antibiotic exposure (Abx), Lactobacillus plantarum PC-170 (PC), and Lactobacillus rhamnosus GG (LGG) group and the Abx, LGG, and PC group received an one-week antibiotic/antibiotic + probiotic treatment. The fecal microbiota and the expression of splenic cytokines were determined. Following the ceftriaxone treatment, the body weight gain of Abx was delayed compared with others. The ceftriaxone treatment significantly decreased the alpha-diversity of the fecal microbiota and altered the fecal microbiota but LGG and PC can partly alleviate the effect. At the end of the study, the microbial community of LGG and PC group were more similar to Ctrl compared with Abx group. The results indicated that ceftriaxone could significantly alter intestinal microbiota. Lactobacilli might alleviate the side effects of antibiotics by stabilizing the intestinal microbiota.

Long-Term Exposure to Ceftriaxone Sodium Induces Alteration of Gut Microbiota Accompanied by Abnormal Behaviors in Mice

“…Ceftriaxone administration caused significant weight loss in the study. This is consistent with the previous finding that the weight gain of mice was delayed significantly following the ceftriaxone treatment (Miao et al, 2020 ). However, it was contradictory with previous findings that antibiotics result in weight gain in the animal production system (Angelakis, 2017 ).…”

“…The gut microbiota of mice was altered greatly in quantity and quality by the oral administration of ceftriaxone in this study. Similar to the result, other studies confirmed that oral ceftriaxone significantly decreased the quantity of fecal microbiota (Cheng et al, 2017 , 2019 ; Guo et al, 2017 ; Miao et al, 2020 ). At the phylum level, the microbiota diversity of the AB group decreased, Proteobacteria became a dominant phylum, and the abundance of Bacteroidetes, Firmicutes, Actinobacteria , and Deferribacteres decreased.…”

“…At the phylum level, the microbiota diversity of the AB group decreased, Proteobacteria became a dominant phylum, and the abundance of Bacteroidetes, Firmicutes, Actinobacteria, and Deferribacteres decreased. This result is supported by studies that ceftriaxone could characteristically decrease the alpha-diversity of the fecal microbiota accompanied with more Proteobacteria and less Bacteroidetes (Cheng et al, 2017(Cheng et al, , 2019Miao et al, 2020). In some dysbiosis and related diseases, an increased Proteobacteria is perceived as a diagnostic characteristic since it is closely related to colon epithelial oxygenation as well as the disruption of the FIGURE 10 | Concentrations of (A) IL-6, (B) IL-10, and (C) corticosterone in serum.…”

“…gut anaerobic environment (Zhu et al, 2013;Shin et al, 2015;Miao et al, 2020). Firmicutes has become a controversial strain as some studies identified an increase in Firmicutes after ceftriaxone treatment, but others have demonstrated a declined Firmicutes in the ceftriaxone group (Cheng et al, 2017(Cheng et al, , 2019Miao et al, 2020). Evidence from clinics has suggested that patients with depression often have decreased Firmicutes (Huang et al, 2018).…”

Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate

“…For the genus level, it has been reported that Lactobacillus and Blautia are probiotics in the intestine. Lactobacillus can inhibit or reduce the invasion of pathogenic bacteria and the production of intestinal inflammatory factors through adhesion and competition [42]. Blautia is an important Gram-positive bacterium that maintains intestinal homeostasis and can produce butyric acid and other beneficial metabolites and prevent inflammation by upregulating the proliferation of intestinal Treg cells [25].…”