Antibiotic Susceptibility Patterns of Propionibacterium Acnes Isolated from Acne Vulgaris in Assiut University Hospitals , Egypt

Abstract

Background: Antibiotics are frequently used to treat acne patients either as bactericidal or anti-inflammatory agents. However, with the increased use of antibiotics, resistant strains of Propionibacterium acnes began to emerge and have been associated with a poor treatment outcome. Objectives: Detection of staphylococcal and Propionibacterium acnes strains in cases of acne vulgaris in Assiut university hospitals, Egypt and antibiotic susceptibility patterns of Propionibacterium acnes isolates. Methodology: Microbiological samples were obtained from one hundred patients with inflammatory acne lesions. Samples were cultured on blood agar and mannitol salt agar media under aerobic conditions at 37°C for isolation of staphylococcal strains, and on blood agar under anaerobic conditions at 37°C for 3 to 7 days for isolation of Propionibacterium acnes. Bacteria were identified by colonial morphology, standard biochemical tests, and API 20A test for identification of Propionibacterium acnes isolates. Antibiotic sensitivity testing of Propionibacterium acnes strains was done against clindamycin, erythromycin, doxycyclin, trimethoprime/sulfamethxazole, tetracycline and levofloxacin. Results: Staphylocoocal strains were detected in 55% of acne cases, while Propionibacterium acnes were detected in 35% of cases. Most Propionibacterium acnes isolates were sensitive to levofloxacin (80%), followed by doxycycline (51.4%), tetracycline, trimethoprime/ sulfamethaxazole (20.0% for each) while showed highest resistance rates to clindamycin (85.7%) and erythromycin (82.9%). Conclusion: Levofloxacin was the most effective antibiotic for Propionibacterium acnes followed by doxycycline, while Erythromycin and clindamycin were the least effective antibiotics for Propionibacterium acnes.

Detection of Anaerobic Bacteria in Bronchoalveolar Lavage of Patients with Chronic Chest Lesion

“…Khassebaf and coleagues [17] reported Propionobacterium resistance 100% to metronidazole and 10% to clindamycin. On the other hand Hassan and colleagues [18] reported high Propionobacterium resistance to clindamycin (70%).…”

Kepekaan Mikrobiota Akne Terhadap Antibiotik Pada Pelajar SMA Penderita Akne Derajat Sedang-Berat Di Mataram, Nusa Tenggara Barat

“…1,2 Akne vulgaris (AV) memiliki patogenesis yang kompleks dan multifaktor, yang terdiri dari peningkatan produksi sebum, hiperproliferasi epidermal duktus piloseba-sea, proses inflamasi dan kolonisasi mikrobiota kulit terutama Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus dan Malassezia furfur. 3,4 Mikrobiota tersebut merupakan bakteri yang paling sering didapatkan dari isolasi lesi akne karena mampu mengkolonisasi duktus folikuler dan bermultiplikasi serta bersifat patogenik terhadap terjadinya akne, skar dan hiperpigmentasi paska inflamasi. 5,6 Beberapa penelitian melaporkan bahwa akne memiliki efek besar pada kualitas hidup pasien (Quality of Life/QoL) dengan mempengaruhi psikologis penderita berupa kecemasan, depresi, emosi dan kurangnya rasa percaya diri akibat dari lamanya akne yang diderita, derajat akne maupun skar akne yang ditimbulkan.…”

Kepekaan Mikrobiota Akne Terhadap Antibiotik Pada Pelajar SMA Penderita Akne Derajat Sedang-Berat Di Mataram, Nusa Tenggara Barat

“…1,2 Akne vulgaris (AV) memiliki patogenesis yang kompleks dan multifaktor, yang terdiri dari peningkatan produksi sebum, hiperproliferasi epidermal duktus piloseba-sea, proses inflamasi dan kolonisasi mikrobiota kulit terutama Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus dan Malassezia furfur. 3,4 Mikrobiota tersebut merupakan bakteri yang paling sering didapatkan dari isolasi lesi akne karena mampu mengkolonisasi duktus folikuler dan bermultiplikasi serta bersifat patogenik terhadap terjadinya akne, skar dan hiperpigmentasi paska inflamasi. 5,6 Beberapa penelitian melaporkan bahwa akne memiliki efek besar pada kualitas hidup pasien (Quality of Life/QoL) dengan mempengaruhi psikologis penderita berupa kecemasan, depresi, emosi dan kurangnya rasa percaya diri akibat dari lamanya akne yang diderita, derajat akne maupun skar akne yang ditimbulkan.…”