Antibiotic Susceptibility of Streptococcus Pyogenes Strains Isolated From Throat Cultures of Children With Tonsillopharyngitis

Çıftçı, Ergın

doi:10.1501/jms_0000000039

Cited by 5 publications

(3 citation statements)

References 20 publications

(24 reference statements)

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“…The positivity rate in our study also matches the study conducted by Rathi et al 17 in Karachi which had 25.3% positivity rate with 60.7% of subjects under 15 years of age and 3.3% above 45 years which is in contrast to our study where infection under 15 is 36% and above 45 is 32%. However, it is almost same for subjects between 15-45 years.…”

Section: Discussionsupporting

confidence: 89%

Prevalence of Microorganisms in Acute Pharyngitis from Throat Swab in a tertiary care hospital

Umar¹,

Baig²,

Arif³

et al. 2021

PJMHS

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Background: Acute pharyngitis is the inflammation of the mucous membrane of oropharynx which is caused by various infectious organisms like bacteria, viruses and fungi. The other less common seen causes include allergies and gastro-esophageal reflux disease. Aim: To look for range of microorganisms involved in causing acute pharyngitis on throat swab specimens. Methods: After getting consent all the pharyngitis patients attending ENT Department at Akbar Niazi Teaching Hospital (ANTH), Islamabad from January to June 2017 were included in the study. From total of 100 patients throat swabs were obtained by established aseptic method and were sent for culture. The organisms involved were recognized by biochemical tests & their propensity to antimicrobial agents was established by established methods. Data was entered and analyzed by using SPSS v.21. Results: Out of 100 specimens’ microorganisms were identified in 25 specimens while 75 specimens had no growth. Streptococcus pyogenes was the frequently seen organism 9(90%), while the list was followed by Staphylococcus aureus 14(56%) and Candida albicans 3(12%). In 40% single pathogen was identified with Streptococcus pyogenes in 9 samples (90%) and Staphylococcus aureus in 1(10%), whereas 60% of the culture reports were that of the mix infection. Conclusion: The study concluded that streptococcus pyogenes and staphylococcus aureus were responsible for majority of single organism infections, while candida albicans together with these bacteria were isolated from mixed infections. Keywords: Pharyngitis; Streptococcus pyogenes; Throat; Microorganisms.

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Section: Discussionsupporting

confidence: 89%

Prevalence of Microorganisms in Acute Pharyngitis from Throat Swab in a tertiary care hospital

Umar¹,

Baig²,

Arif³

et al. 2021

PJMHS

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“…Firstly, the susceptibility of planktonic M12 GAS to penicillin was confirmed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values (data not shown). A MIC and MBC of 0.025 μg/mL was established, which is consistent with the reported GAS MIC and MBC range for penicillin [36,37]. Notably, minimum biofilm eradication concentration values (MBECs) determined for M12 GAS biofilms formed on untreated and exoglycosidase pre-treated monolayers (Table 1) were all considerably higher than the MIC for planktonic M12 GAS, resulting in 2500-5000-fold greater penicillin tolerance.…”

Section: Increased Biofilm Formation Promotes Penicillin Tolerancesupporting

confidence: 83%

Assessing the Role of Pharyngeal Cell Surface Glycans in Group A Streptococcus Biofilm Formation

et al. 2020

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Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Antibiotic treatment failure rates of 20–40% have been observed. The role host cell glycans play in GAS biofilm formation in the context of GAS pharyngitis and subsequent antibiotic treatment failure has not been previously investigated. GAS serotype M12 GAS biofilms were assessed for biofilm formation on Detroit 562 pharyngeal cell monolayers following enzymatic removal of all N-linked glycans from pharyngeal cells with PNGase F. Removal of N-linked glycans resulted in an increase in biofilm biomass compared to untreated controls. Further investigation into the removal of terminal mannose and sialic acid residues with α1-6 mannosidase and the broad specificity sialidase (Sialidase A) also found that biofilm biomass increased significantly when compared to untreated controls. Increases in biofilm biomass were associated with increased production of extracellular polymeric substances (EPS). Furthermore, it was found that M12 GAS biofilms grown on untreated pharyngeal monolayers exhibited a 2500-fold increase in penicillin tolerance compared to planktonic GAS. Pre-treatment of monolayers with exoglycosidases resulted in a further doubling of penicillin tolerance in resultant biofilms. Lastly, an additional eight GAS emm-types were assessed for biofilm formation in response to terminal mannose and sialic acid residue removal. As seen for M12, biofilm biomass on monolayers increased following removal of terminal mannose and sialic acid residues. Collectively, these data demonstrate that pharyngeal cell surface glycan structures directly impact GAS biofilm formation in a strain and glycan specific fashion.

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“…Çiftçi ve ark. (8) 2003 yılında boğaz kültürlerinden izole edilen 263 S.pyogenes'in 10'unda (% 3.8) eritromisin direnci bulmuşlardır. Eryılmaz ve ark.…”

Section: Bulgularunclassified

Erythromycin Resistance in Streptococcus pyogenes: 2009-2013

KARAPINAR¹,

Yılmaz²,

ÖZBAYRAK³

et al. 2015

Ankem Derg

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Streptococcus pyogenes pharyngitis is a common childhood disease. The aim of this retrospective study is to evaluate proportion of macrolide resistance among pharyngeal isolates of S.pyogenes from pediatric patients between 01.01.2009 and 31.12.2013. The strains were isolated and identified by conventional methods. The antibiotic susceptibility testing was performed by disc diffusion according to Clinical and Laboratory Standards Institude standards. We studied 12,597 throat cultures and were isolated 1,159 (9.2 %) S.pyogenes in five years. In these isolates, erythromycin resistance rates over the years were

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Antibiotic Susceptibility of Streptococcus Pyogenes Strains Isolated From Throat Cultures of Children With Tonsillopharyngitis

Cited by 5 publications

References 20 publications

Prevalence of Microorganisms in Acute Pharyngitis from Throat Swab in a tertiary care hospital

Prevalence of Microorganisms in Acute Pharyngitis from Throat Swab in a tertiary care hospital

Assessing the Role of Pharyngeal Cell Surface Glycans in Group A Streptococcus Biofilm Formation

Erythromycin Resistance in Streptococcus pyogenes: 2009-2013

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