Antibiotic susceptibility of Staphylococci isolated from bovine mastitis in Algeria

Saidi, Radhwane; Mimoune, Nora; Baazizi, Ratiba; Benaissa, Mohammed Hocine; Khelef, Djamel; Kaidi, Rachid

doi:10.5455/javar.2019.f337

Cited by 20 publications

(20 citation statements)

References 10 publications

(9 reference statements)

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“…Carriage of MRSA in healthy poultry was reported and emphasized that antibiotic resistance was higher in the recent isolates than prior isolates [ 6 ]. A high percentage of methicillin resistance in Staphylococci isolated from bovine mastitis was also reported in Algeria [ 7 ].…”

Section: Introductionmentioning

confidence: 91%

Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

Bounar-Kechih¹,

Hamdi

Aggad

et al. 2018

Veterinary Medicine International

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Multiresistant and especially Methicillin-Resistant Staphylococcus aureus (MRSA) poses a serious public health problem that requires their immediate identification and antibiotic resistance characteristics. In order to determine antibiotic resistance S. aureus poultry and bovine origin, 8840 samples were collected from slaughterhouses in the northern region of Algeria between years 2009 and 2014. 8375 samples were from an avian origin (1875 from laying hens and 6500 from broiler chickens) and the rest was from bovine origin. Bacteriological isolation and identification were made by classical culture method and antibiotic resistance patterns were determined by disc diffusion test. The prevalence of S. aureus was 42% in laying hens, 12% in broilers, and 55% in bovine samples. The prevalence of MRSA was 57%, 50%, and 31% in laying hens, broiler chickens, and bovine, respectively. While MRSA strains isolated from poultry showed cross-resistance to aminoglycosides, fluoroquinolones, macrolides, sulphonamides, and cyclins, those isolated from bovine also revealed similar multiresistance except for sulphonamide. This high percentage of methicillin resistance and multidrug resistance in S. aureus poultry and bovine origin may have importance for human health and curing of human infections.

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Section: Introductionmentioning

confidence: 91%

Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

Bounar-Kechih¹,

Hamdi

Aggad

et al. 2018

Veterinary Medicine International

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“…In all clinical isolates, the resistance rate of S. aureus to gentamicin was 7.7% which was similar (7.0%) with a study performed in Algeria [ 25 ]. In Algeria, no resistance to gentamicin was found in staphylococci in milk [ 54 , 55 ]. One S. aureus isolate exhibiting phenotypic resistance to gentamicin harboured the aac A- aph D gene which is in contrast to previous studies [ 16 , 33 ].…”

Section: Main Textmentioning

confidence: 99%

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

et al. 2018

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ObjectiveThe antimicrobial resistance of staphylococci rose worldwide. In total, 96 Staphylococcus isolates from food and clinical samples were collected from two provinces in Algeria. The antimicrobial susceptibility testing was performed and resistance-associated genes were detected.ResultsFifty-one strains were isolated from food samples and differentiated into 33 Staphylococcus aureus and 18 coagulase-negative staphylococci. Forty-five staphylococci were collected from hospital and community-acquired infection cases. All S. aureus isolated from food were resistant to penicillin and 45.5% were resistant to tetracycline. The resistance rates of 45 clinical Staphylococcus isolates were 86.7%, 48.9%, 37.8% and 20.0% to penicillin, tetracycline, erythromycin and kanamycin, respectively. Nine isolates were confirmed as MRSA from food and clinical isolates. One S. aureus originated from food was confirmed as vancomycin-resistant. Multidrug-resistance was observed among 25.5% and 53.3% of food and clinical staphylococci, respectively. The tetM/K, blaZ, aacA-aphD, ermC and mecA genes were detected in food and clinical isolates. ermA gene was not found. This study provided insight into the status of antimicrobial resistance of staphylococci isolated from food and clinical samples in Algeria. Further investigations and surveillance programmes are mandatory.

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“…12 Multi-drug resistant strains are resistant to three or more groups of antibiotics. 13 In this study S aureus ATCC 25923 (penicillin-susceptible strain), S aureus ATCC 29213 (penicillin resistant, beta-lactamase positive strain), and MRSA ATCC 35591 (positive for both the mecA and PVL genes) were used as a control or reference strains.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Characterization, and Detection of PVL Encoding Gene in Methicillin Resistant Staphylococcus Aureus Strains Isolated From Patients Admitted to a Tertiary Hospital In Kuantan, Malaysia

Bariman¹,

Mahmoud²,

Hamzah³

2020

imjm

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Introduction: Methicillin-resistant Staphylococcus aureus is globally a major public health threat. Resistance to methicillin originates from a modified protein (PBP2a) encoded by the mecA gene. The PVL gene as an important virulence factor increases the pathogenicity of MRSA. Epidemiology and characteristics of MRSA differ in different geographical regions. This study was conducted to characterize and determine the antibiotic susceptibility profile of MRSA strains isolated from patients in Hospital Tengku Ampuan Afzan, Pahang, Malaysia and to detect the presence of the mecA and PVL genes in the isolates. Materials and methods: In this study a total of 36 isolates of MRSA have been collected during a period of three months (1stFebruary –30thApril 2018). The susceptibility pattern of the isolates to ten different commonly used antibiotics were determined and the target genes were addressed by real-time PCR experiment. Results: Based on the identifying criteria, 44.4% of the isolates were CA-MRSA, and 55.5% were HA-MRSA. Resistance to oxacillin, cefoxitin and penicillin was 100%, gentamicin 88.8%, erythromycin 33.3%, tetracycline 77.7%, trimethoprim-sulfamethoxazole 61.1%, clindamycin 13.8%, chloramphenicol 11.1%, but no resistant strain of vancomycin was detected. Most of the isolates were resistant to more than three groups of antibiotics. Realtime PCR revealed that all the isolates were mecA positive and 4 isolates were PVL-positive. PVL-positive strains were CA-MRSA and susceptible to clindamycin. Conclusion: The study confirms multi-drug resistant MRSA in the study area, and shows that resistance to methicillin is mecA mediated. PVL carrier strains were present and related to CA-MRSA strains of the isolates.

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Antibiotic susceptibility of Staphylococci isolated from bovine mastitis in Algeria

Cited by 20 publications

References 10 publications

Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

Phenotypic and Genotypic Characterization, and Detection of PVL Encoding Gene in Methicillin Resistant Staphylococcus Aureus Strains Isolated From Patients Admitted to a Tertiary Hospital In Kuantan, Malaysia

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