Antibiotic Susceptibility of Microbial Isolates from Nasal Swabs of Bovine Pneumonic Cases

Katoh, Toshihide; Koya, Masato; Watanabe, Eiji; Sakai, Jun‐ichi; Ogata, Yoshimi; Hikinuma, Tooru

doi:10.12935/jvma1951.49.81

Cited by 7 publications

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“…Kudou et al [10] reported in 1994 that tetracyclines are comparatively effective against M. bovis and M. alkalescens. On the other hand, the efficacy against the same species was rather low in other reports [6,7,13]. The present results support the latter findings.…”

supporting

confidence: 88%

“…The MIC values of enrofloxacin were comparable among M. bovis, M. alkalescens and M. bovoculi in this study. The MIC values of M. bovis and M. alkalescens were similar to those previously reported in Japan and elsewhere [5][6][7]13]. The MIC value of enrofloxacin was higher for M. bovirhinis than for other species.…”

supporting

confidence: 88%

“…Because vaccination of cattle to prevent Mycoplasma infection has not yet been approved in Japan, other approaches are needed, such as preventing bacterial invasion of farms through hygiene management, preventing respiratory symptoms through nutritional management and antibiotic treatment of cattle with abnormal respiratory manifestations. Although the antimicrobial susceptibility of Mycoplasma species has changed [6,7,10], there has been no report of examination of the susceptibility in Japan since isolates were collected from 1996-1997 [6]. Therefore, this study examined the antimicrobial susceptibility of Mycoplasma strains recently isolated from cattle in Japan.…”

mentioning

confidence: 99%

“…72(12): 1661-1663, 2010 Bovine respiratory disease complex (BRDC) caused by several combinations of viral and bacterial infections together with stress is a significant problem that has considerable economic impact on the cattle industry [4,12]. Mycoplasma is a causative agent of BRDC [2,6,7,10,12]. Because vaccination of cattle to prevent Mycoplasma infection has not yet been approved in Japan, other approaches are needed, such as preventing bacterial invasion of farms through hygiene management, preventing respiratory symptoms through nutritional management and antibiotic treatment of cattle with abnormal respiratory manifestations.…”

mentioning

confidence: 99%

“…Mycoplasma is a causative agent of BRDC [2,6,7,10,12]. Because vaccination of cattle to prevent Mycoplasma infection has not yet been approved in Japan, other approaches are needed, such as preventing bacterial invasion of farms through hygiene management, preventing respiratory symptoms through nutritional management and antibiotic treatment of cattle with abnormal respiratory manifestations.…”

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confidence: 99%

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Antimicrobial Susceptibilities of Four Species of Mycoplasma Isolated in 2008 and 2009 from Cattle in Japan

2010

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ABSTRACT. We examined the susceptibility in vitro of 99 isolates of Mycoplasma (M. bovirhinis, M. bovis, M. alkalescens and M. bovoculi) and one of Acholeplasma laidlawii collected in 2008 and 2009 from Japanese homebred cattle and cows imported from Australia to 13 antimicrobial agents. Mycoplasma species isolated from homebred cattle were susceptible to spectinomycin and fluoroquinolones but resistant to macrolides, tetracyclines, thiamphenicol and flumequine. Kanamycin, lincomycin and chloramphenicol maintained intermediate effectiveness against these species. The isolates from the imported cows maintained sufficient susceptibility to all antimicrobials examined except for erythromycin and thiamphenicol. These results showed that four species of Mycoplasma isolated from Japanese homebred cattle had obviously higher drug resistance compared with previous reports, even within this decade.KEY WORDS: bovine respiratory diseases, drug resistance, Mycoplasma spp.J. Vet. Med. Sci. 72(12): 1661-1663, 2010 Bovine respiratory disease complex (BRDC) caused by several combinations of viral and bacterial infections together with stress is a significant problem that has considerable economic impact on the cattle industry [4,12]. Mycoplasma is a causative agent of BRDC [2,6,7,10,12]. Because vaccination of cattle to prevent Mycoplasma infection has not yet been approved in Japan, other approaches are needed, such as preventing bacterial invasion of farms through hygiene management, preventing respiratory symptoms through nutritional management and antibiotic treatment of cattle with abnormal respiratory manifestations. Although the antimicrobial susceptibility of Mycoplasma species has changed [6,7,10], there has been no report of examination of the susceptibility in Japan since isolates were collected from 1996-1997 [6]. Therefore, this study examined the antimicrobial susceptibility of Mycoplasma strains recently isolated from cattle in Japan.We analyzed 99 isolates of Mycoplasma (M. bovirhinis, M. bovis, M. alkalescens and M. bovoculi) and one of Acholeplasma laidlawii collected in 2008 and 2009 from Japanese homebred cattle that were 15 days to 11 months old at 33 farms in Kyusyu and healthy cows imported from Australia that were 14 to 15 months old. At the time of sampling, the Australian cattle were isolated from other cows because of quarantine restrictions at the destination farm.We examined the minimum inhibitory concentrations (MICs) of 13 antimicrobial agents (kanamycin, spectinomycin, erythromycin, tylosin, tilmicosin, lincomycin, oxytetracycline, chlortetracycline, chloramphenicol, thiamphenicol, flumequine, enrofloxacin and danofloxacin) required to completely suppress the growth of the four species of Mycoplasma and A. laidlawii. The MICs were determined using agar dilution according to the Clinical and Laboratory Standards Institute (CLSI) guidelines [3] and a previous report [5]. Mycoplasma species and A. laidlawii were grown and diluted 1:100 in M broth [9]. Bacterial suspensions (about 10 6 colony formi...

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supporting

confidence: 88%

supporting

confidence: 88%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Antimicrobial Susceptibilities of Four Species of Mycoplasma Isolated in 2008 and 2009 from Cattle in Japan

2010

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Mycoplasma bovispneumonia in cattle

Caswell

Archambault

2007

Anim. Health. Res. Rev.

162

189

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Mycoplasma bovis is an important and emerging cause of respiratory disease and arthritis in feedlot cattle and young dairy and veal calves, and has a variety of other disease manifestations in cattle. M. bovis is certainly capable of causing acute respiratory disease in cattle, yet the attributable fraction has been difficult to estimate. In contrast, M. bovis is more accepted as a cause of chronic bronchopneumonia with caseous and perhaps coagulative necrosis, characterized by persistent infection that seems poorly responsive to many antibiotics. An understanding of the disease has been recently advanced by comparisons of natural and experimentally induced disease, development of molecular diagnostic tools, and understanding some aspects of virulence, yet uncertainties regarding protective immunity, the importance of genotypic diversity, mechanisms of virulence, and the role of co-pathogens have restricted our understanding of pathogenesis and our ability to effectively control the disease. This review critically considers the relationship between M. bovis infection and the various manifestations of the bovine respiratory disease complex, and addresses the pathogenesis, clinical and pathologic sequelae, laboratory diagnosis and control of disease resulting from M. bovis infection in the bovine respiratory tract.

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Isolation of Mycoplasmas from Nasal Swabs of Calves affected with Respiratory Diseases and Antimicrobial Susceptibility of their Isolates

Hirose

Kobayashi

Ito

et al. 2003

Journal of Veterinary Medicine, Series B

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Nasal swabs of 293 calves were examined for Mycoplasma. The samples were collected from calves affected with respiratory diseases on 71 farms in various parts of Japan between 1996 and 1997. Mycoplasma bovirhinis was isolated from 47 of 293 calves (16.0%). Mycoplasma alkalescens, M. bovis, M. arginini, M. bovigenitalium and Acholeplasma spp. were isolated from 19 (6.5%), seven (2.4%), four (1.4%), four (1.4%) and 18 (6.1%) calves, respectively. Pasteurella multocida and P. haemolytica were isolated from 60% of Mycoplasma-positive calves. However, other bacteria were not isolated from calves. To evaluate the antimicrobial susceptibility of their isolates, 68 M. bovirhinis, 21 M. alkalescens and 10 M. bovis strains were examined for 12 antimicrobial agents. All isolates showed higher susceptibility to tiamulin than to the other drugs used in the study. However, erythromycin had no effect on any of the Mycoplasma strains studied. The field isolates were less susceptible than the type strains to some drugs, such as spiramycin, oxytetracycline and tylosin.

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Antibiotic Susceptibility of Microbial Isolates from Nasal Swabs of Bovine Pneumonic Cases

Cited by 7 publications

References 0 publications

Antimicrobial Susceptibilities of Four Species of Mycoplasma Isolated in 2008 and 2009 from Cattle in Japan

Antimicrobial Susceptibilities of Four Species of Mycoplasma Isolated in 2008 and 2009 from Cattle in Japan

Mycoplasma bovispneumonia in cattle

Isolation of Mycoplasmas from Nasal Swabs of Calves affected with Respiratory Diseases and Antimicrobial Susceptibility of their Isolates

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