Antibiotic susceptibility of bacterial isolates from probiotic products available in Italy

Blandino, G; Milazzo, I.; Fazio, Davide

doi:10.1080/08910600802408111

Cited by 19 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These organisms have the potential to act as reservoir of antimicrobial resistant genes and which have potential to be transferred to other bacteria. Moreover, intrinsic resistance to vancomycin was confirmed for L. paracasei, L. salivarius and L. plantarum, and L. salivarius was also typically resistant to erythromycin [ 31 ]. In other study, L. salivarius had the highest resistance to kanamycin and neomycin, but demonstrated the lowest resistance to penicillin [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia

et al. 2014

View full text Add to dashboard Cite

BackgroundProbiotics are commonly used as feed additive to substitute antibiotic as growth promoter in animal farming. Probiotic consists of lactic acid bacteria (LAB), which enhance the growth and health of the animal. Probiotic also have higher possibility to become pathogenic bacteria that may carry antibiotic resistant gene that can be transmitted to other LAB species. The aim of this study was to identify the LAB species in the faeces of broiler chicken and to determine the prevalence of antibiotic resistant in LAB of broiler chicken.MethodsSixty faeces samples were collected from wet markets located in Klang Valley of Malaysia for the isolation of LAB using de-Mann Rogosa Sharpe medium. Thirteen species of LAB were obtained in this study and the identification of LAB was performed by using API test kit on the basis of carbohydrate fermentation profile. Antibiotic susceptibility assay was then carried out to determine the prevalence of LAB antibiotic resistance.ResultsLactococcus lactis subsp lactis was found in nine out of sixty faecal samples. Lactobacillus paracasei was the second common LAB species isolated from chicken faecal. No significant difference (P > 0.05) was found between the occurrence of Lactobacillus brevis, Lactobacillus curvatus, Lactobacillus plantarum, Leuconostoc lactis mesenteroides subsp mesenteroides/dectranium and Pediococcus pentosaceus isolated from 5 different locations. Most of the isolated LAB was resistant to antibiotic and high variability of the antibiotic resistance was observed among the LAB against 15 types of antibiotics. Penicillin, amoxicillin, chloramphenicol, and ampicillin had significant higher (P< 0.05) inhibitory zone than nalidixic acid, gentamycin, sulphamethoxazole, kanamycin, and streptomycin.ConclusionsMany species of LAB were isolated from the faecal samples of broiler chicken that resistance to the common antibiotics used in the farm. The development of resistant against antibiotics in LAB can be attributed to the long term exposure of antibiotic as growth promoter and therapeutic agents. Thus, it is essential to advise farmer the safety measure of antibiotic application in animal farming. Additionally, the supplementation of probiotic in animal feeding also needs more attention and close monitoring.

show abstract

Section: Resultsmentioning

confidence: 99%

Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The antimicrobial susceptibility patterns for L. salivarius isolated from multiple sources have been reported, however scarcely. For example, Blandino et al (2008) studied the AMR profiles of LAB isolated from probiotic products in Italy and found that one strain of L. salivarius was atypically resistant to erythromycin. The authors indicated that this was a nonintrinsic case of resistance, compared to the resistance level of other LAB in the study (Blandino et al 2008).…”

Section: Antimicrobial Resistance Of L Salivariusmentioning

confidence: 99%

“…For example, Blandino et al (2008) studied the AMR profiles of LAB isolated from probiotic products in Italy and found that one strain of L. salivarius was atypically resistant to erythromycin. The authors indicated that this was a nonintrinsic case of resistance, compared to the resistance level of other LAB in the study (Blandino et al 2008). Conversely, Langa et al (2012) reported that L. salivarius CECT 5713 isolated from human milk was sensitive to most antibiotics tested and no transmissible genes potentially involved in antibiotic resistance were detected (Langa et al 2012).…”

Section: Antimicrobial Resistance Of L Salivariusmentioning

confidence: 99%

Applications and safety considerations ofLactobacillus salivariusas a probiotic in animal and human health

2017

View full text Add to dashboard Cite

SummaryThe goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal health, particularly to reduce colonization by gastrointestinal pathogens, and to a lesser extent, as a production and quality aid. In humans, L. salivarius has been used to prevent and treat a variety of chronic diseases, including asthma, cancer, atopic dermatitis and halitosis, and to a much limited extent, to prevent or treat infections. Based on the results from primary research evidence, it seems that L. salivarius does not pose a health risk to animals or humans in the doses currently used for a variety of applications; however, there is a systematic lack of studies assuring the safety of many of the strains intended for clinical use. This review provides researchers in the field with up-to-date information regarding applications and safety of L. salivarius. Furthermore, it helps researchers identify knowledge gaps and potential opportunities for microbiological and clinical research.

show abstract

“…In a few species, the locus is only composed of the TCS predicted operon. The first and the third configurations are observed in vancomycin-resistant Lactobacillus species (Mackey et al, 1993;Wilks et al, 2004;Klare et al, 2007;Balandino et al, 2008) except for L. fermentum which displays a variable strain-dependent vancomycin resistance (Rossetti et al, 2009). The second configuration is only observed in the vancomycinsusceptible species.…”

Section: Predictive Analysis Of Operon Cwars-ldca and Polar Effect Ofmentioning

confidence: 96%

Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei

et al. 2020

View full text Add to dashboard Cite

The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the gut. The genes cwaR and cwaS encode a predicted two-component system (TCS) and ldcA a predicted D-alanyl-D-alanine carboxypeptidase which is a peptidoglycan (PG) biosynthesis enzyme. We explored the functionality and the physiological role of these three genes, particularly their impact on the bacterial cell wall architecture and on the bacterial adaptation to environmental perturbations in the gut. The functionality of CwaS/R proteins as a TCS has been demonstrated by biochemical analysis. It is involved in the transcriptional regulation of several genes of the PG biosynthesis. Analysis of the muropeptides of PG in mutants allowed us to re-annotate LSEI_0221 as a putative L,D-carboxypeptidase (LdcA). The absence of this protein coincided with a decrease of two surface antigens: LSEI_0020, corresponding to p40 or msp2 whose implication in the host epithelial homeostasis has been recently studied, and LSEI_2029 which has never been functionally characterized. The inactivation of each of these three genes induces susceptibility to antimicrobial peptides (hBD1, hBD2, and CCL20), which could be the main cause of the gut establishment deficiency. Thus, this operon is necessary for the presence of two surface antigens and for a suitable cell wall architecture.

show abstract

Antibiotic susceptibility of bacterial isolates from probiotic products available in Italy

Cited by 19 publications

References 5 publications

Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia

Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia

Applications and safety considerations ofLactobacillus salivariusas a probiotic in animal and human health

Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei

Contact Info

Product

Resources

About