Antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat

Abstract

The zoonotic potential of Escherichia coli from chicken and beef food products is well documented. The uses of antibiotics on agriculture encourage the development of resistance bacteria capable of causing human disease and passing resistance to human pathogens. This study aimed to detect the antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat. E. coli was isolated and identified according to standard techniques using traditional and chromogenic media and confirmed by biochemical reaction. Kirby-Bauer disk diffusion method was used to determine antimicrobial susceptibility towards twelve commonly used antibiotics. The resistance of the isolated E. coli towards the third generation of cephalosporins was detected using cefotaxime (30µg), ceftriaxone (30 µg) and ceftazidime (30 µg). ESBL producer E. coli was investigated using combination test. The results showed that 135 (75%) of the 180 meat samples revealed positive isolation of E. coli. 77.33% of the chicken meat samples showed positive isolation of E. coli, while 63.33% (19/30) of minced beef meat samples showed positive growth of E. coli. From these isolates, it was clear that most of them were highly resistant to tetracycline (10 µg), amoxiclav (30 µg) and cefalexin (30 µg). The lowest resistance was observed with ceftriaxone (30 µg) and ceftazidime (30 µg). The resistance of the isolated E. coli towards the third generation of cephalosporins was ranged between 5 to 33%. This study revealed that the isolated E. coli was ESBL producer as 85.71, 83.33, 70.83, 68.18 and 66.66% were detected in chickens leg, skin, wing, abdomen and chest respectively; while minced meat showed isolation of 15.78% of the ESBL producer E. coli. The study concluded that chicken and beef minced meat sold in Khartoum state have high hazardous risk for transmission of ESBLs producing E. coli; thus quality control application is highly needed. Policy actions should be implemented in order to prevent cross transmission of ESBLs producer E. coli to human.

Antimicrobial Resistance and Molecular Detection of Extended

“…Antimicrobial use in production animals has been shown to lead to the emergence of resistant Enterobactericeae throughout the food chain (Aworh et al, 2020). Most likely, the use of low doses of antibiotics by the modern food animal industry as growth-promoting substances in farm animals to promote animal growth and to prevent infections rather than cure infections is responsible for drug-resistant bacteria emerging on farms which reach the general population through human or animal carriers, and through the food consumers eat (Omer et al, 2021). So, the misuse and overuse of broad-spectrum antibiotics, mainly cephalosporins, must be contributing to selection and spread of ESBL-producing Enterobacteriaceae in animals (Aworh et al, 2020).…”

“…Extended spectrum beta-lactamase (ESBL) producing E. coli, which is zoonotic in nature, is one of the commonest resistant pathogens causing human infections (Aworh et al, 2020). It has been reported in several studies that antimicrobial resistance among E. coli isolates has increased globally mainly as a result of the high prevalence of ESBL producing bacteria (Abayneh et al, 2019;Aworh et al, 2020;Omer et al, 2021). This high prevalence of ESBL producing E. coli has resulted from growing reservoirs in animals especially their product that is commonly consumed and the use of antimicrobials Aworh et al, 2020).…”