Antimicrobial Resistance and Molecular Detection of Extended

Abstract

Escherichia coli (E. coli) are common etiological agents of food borne diseases. A study on antimicrobial resistance and molecular detection of Extended-Spectrum β-lactamase resistance (ESBL) in E. coli from road-side suya meat was carried out. Suya meat (50 each from Akwanga, Nasarawa Eggon and Wamba) were collected; and E. coli was isolated and identified using cultural, morphological and biochemical characteristics. Antimicrobial susceptibility testing was carried out using disc diffusion method and interpreted as described by the Clinical and Laboratory Standards Institute. The phenotypic detection of ESBL production in multi-drug resistant isolates was carried using double disc synergy test. Screening for ESBL genes namely blaTEM, blaSHV and blaCTX-M was carried out using polymerase chain reaction (PCR) method. The overall percentage occurrence of the isolates was 11(7.3%), being highest in wamba (10.0%), and lowest in Nasarawa Eggon (4.0%). The isolates were more resistant to amoxicillin/clavulanic acid, cefotaxime, and ceftazidine (54.5%). Percentage occurrence of ESBL producing isolates was 4(36.4%). Multidrug resistance (MDR) isolates (63.6%) occurred more than extensive drug resistance (XDR) (36.4%). The percentage occurrence of ESBL genes namely: blaSHV and blaTEM were 3 (75.0%). The Beta-lactam antibiotics such as amoxicillin/clavulanic acid, ceftazidime and cefotaxime were effective against the isolates and most of the isolates were ESBL resistant.