Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains

Vuthy, Yith; Lay, Kruy Sun; Heng, Seiha; Kerléguer, Alexandra; Aı̈dara-Kane, Awa

doi:10.1016/j.apjtb.2017.07.002

Cited by 26 publications

(24 citation statements)

References 21 publications

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“…The resistance genes analysis carried out here could not successfully explain resistance to macrolides, nitrofurans, and quinolones in our isolates. In fact, phenotypic patterns and genetic detection correlation differences have been described previously ( 70 – 72 ), but point mutations not considered in this study may explain these phenotypes. Additional studies are still needed in order to identify the genes or mutations responsible for antimicrobial resistance in S .…”

Section: Discussionmentioning

confidence: 54%

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

et al. 2020

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Salmonella enterica is one of the most important foodborne pathogens around the world. In the last years, S . enterica serovar Infantis has become an important emerging pathogen in many countries, often as multidrug resistant clones. To understand the importance of S. enterica in the broiler industry in Ecuador, we performed a study based on phenotypic and WGS data of isolates from poultry farms, chicken carcasses and humans. We showed a high prevalence of S. enterica in poultry farms (41.4%) and chicken carcasses (55.5%), but a low prevalence (1.98%) in human samples. S . Infantis was shown to be the most prevalent serovar with a 98.2, 97.8, and 50% in farms, foods, and humans, respectively, presenting multidrug resistant patterns. All sequenced S . Infantis isolates belonged to ST32. For the first time, a pESI-related megaplasmid was identified in Ecuadorian samples. This plasmid contains genes of antimicrobial resistance, virulence factors, and environmental stress tolerance. Genomic analysis showed a low divergence of S . Infantis strains in the three analyzed components. The results from this study provide important information about genetic elements that may help understand the molecular epidemiology of S . Infantis in Ecuador.

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Section: Discussionmentioning

confidence: 54%

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

et al. 2020

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“…A study in Cambodia on sulfamethoxazole-resistant isolates obtained from faecal samples of healthy chickens found a higher prevalence of the sul 2 gene in E. coli (56%) and in Salmonella Corvallis (53%). However, among the Salmonella Albany the most prevalent gene was sul 1 (54%) [58].…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Antibiotic Resistance Genes in Multidrug-Resistant Enterobacteriaceae on Portuguese Livestock Manure

et al. 2019

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The exposure of both crop fields and humans to antibiotic-resistant bacteria in animal excreta is an emergent concern of the One Health initiative. This study assessed the contamination of livestock manure from poultry, pig, dairy farms and slaughterhouses in Portugal with resistance determinants. The resistance profiles of 331 Enterobacteriaceae isolates to eight β-lactam (amoxicillin, cefoxitin, cefotaxime, cefpirome, aztreonam, ceftazidime, imipenem and meropenem) and to five non-β-lactam antibiotics (tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ciprofloxacin (CIP), chloramphenicol (CHL) and gentamicin) was investigated. Forty-nine integron and non-β-lactam resistance genes were also screened for. Rates of resistance to the 13 antibiotics ranged from 80.8% to 0.6%. Multidrug resistance (MDR) rates were highest in pig farm samples (79%). Thirty different integron and resistance genes were identified. These were mainly associated with resistance to CHL (catI and catII), CIP (mainly, qnrS, qnrB and oqx), TET (mainly tet(A) and tet(M)) and SXT (mostly dfrIa group and sul3). In MDR isolates, integron presence and non-β-lactam resistance to TET, SXT and CHL were positively correlated. Overall, a high prevalence of MDR Enterobacteriaceae was found in livestock manure. The high gene diversity for antibiotic resistance identified in this study highlights the risk of MDR spread within the environment through manure use.

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“…Additionally, the growing access to, and the use of, antimicrobials (either through prescription or non-prescription), in both people and animals, leads to an increase in multidrug resistance among several pathogens ( 42 ). Several genes have been linked to AMR in bacteria isolated from backyard animals, humans and even seafood, against tetracycline ( tet A, tet B, tet C, and tet G) ( 43 ), amoxicillin, amoxicillin+clavulanic acid, ampicillin, and ceftiofur ( bla PSE−1 , bla TEM , and bla CMY ) ( 44 ), among other resistance genes related to antimicrobials widely used ( 45 ). Thus, MDR STEC strains have been described as a major public health threat worldwide and in Chile ( 46 , 47 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Usage Factors and Resistance Profiles of Shiga Toxin-Producing Escherichia coli in Backyard Production Systems From Central Chile

et al. 2021

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Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen and important cause of foodborne disease worldwide. Many animal species in backyard production systems (BPS) harbor STEC, systems characterized by low biosecurity and technification. No information is reported on STEC circulation, antimicrobial resistance (AMR) and potential drivers of antimicrobial usage in Chilean BPS, increasing the risk of maintenance and transmission of zoonotic pathogens and AMR generation. Thus, the aim of this study was to characterize phenotypic and genotypic AMR and to study the epidemiology of STEC isolated in BPS from Metropolitana region, Chile. A total of 85 BPS were sampled. Minimal inhibitory concentration and whole genome sequencing was assessed in 10 STEC strain isolated from BPS. All strains were cephalexin-resistant (100%, n = 10), and five strains were resistant to chloramphenicol (50%). The most frequent serotype was O113:H21 (40%), followed by O76:H19 (40%), O91:H14 (10%), and O130:H11 (10%). The stx1 type was detected in all isolated strains, while stx2 was only detected in two strains. The Stx subtype most frequently detected was stx1c (80%), followed by stx1a (20%), stx2b (10%), and stx2d (10%). All strains harbored chromosomal blaAmpC. Principal component analysis shows that BPS size, number of cattle, pet and horse, and elevation act as driver of antimicrobial usage. Logistic multivariable regression shows that recognition of diseases in animals (p = 0.038; OR = 9.382; 95% CI: 1.138–77.345), neighboring poultry and/or swine BPS (p = 0.006; OR = 10.564; 95% CI: 1.996–55.894), visit of Veterinary Officials (p = 0.010; OR = 76.178; 95% CI: 2.860–2029.315) and close contact between animal species in the BPS (p = 0.021; OR = 9.030; 95% CI: 1.385–58.888) increase significantly the risk of antimicrobial use in BPS. This is the first evidence of STEC strains circulating in BPS in Chile, exhibiting phenotypic AMR, representing a threat for animal and public health. Additionally, we identified factors acting as drivers for antimicrobial usage in BPS, highlighting the importance of integration of these populations into surveillance and education programs to tackle the potential development of antimicrobial resistance and therefore the risk for ecosystemic health.

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Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains

Cited by 26 publications

References 21 publications

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

Genomic Epidemiology of Salmonella Infantis in Ecuador: From Poultry Farms to Human Infections

Prevalence of Antibiotic Resistance Genes in Multidrug-Resistant Enterobacteriaceae on Portuguese Livestock Manure

Antimicrobial Usage Factors and Resistance Profiles of Shiga Toxin-Producing Escherichia coli in Backyard Production Systems From Central Chile

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