Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping ofStaphylococcus aureusIsolated in Sicily, Italy

Abstract

Staphylococcus aureus is the major cause of foodborne diseases worldwide. In this retrospective study, 84 S. aureus strains were characterized. The collection comprises 78 strains isolated during 1998 and 2014 from dairy products and tissue samples from livestock bred for dairy production in Sicily. One isolate was obtained from a pet (dog), one from an exotic animal (a circus elephant), and four human isolates were obtained during a severe food poisoning outbreak that occurred in Sicily in 2015. All the strains were characterized by pulsed-field gel electrophoresis (PFGE), for antibiotic resistance and presence of toxin genes. PFGE results showed 10 different pulsotypes, with three relatively frequent and three unique. The antibiotic resistance profiling showed that penicillin G (35.7%) and tetracycline (20.2%) resistance is largely spread. Most isolates contained at least one toxin gene making them a potential threat for public health. Enterotoxin sec gene was observed in 28.6% and seg in 23.8% of the strains, respectively; the human isolates were the only ones to concurrently harbor both seg and sei genes. In addition, 24 isolates were randomly selected and analyzed by multilocus sequence typing. Interestingly, the analysis showed the presence of 12 sequence types (STs), of which 6 were novel. One of them, ST700, was detected in 29% of the isolates and was found to be spread throughout Sicily. ST700 has been present in the island for almost 16 years (1998–2014) and it shows no host preference since it was isolated from different ruminant species. Four human isolates shared both the pulsotype (PT10) and the sequence type (ST9), as well as the virulence genes (seg-sei); this observation suggests that the isolates originated from a single clone, although they were obtained from two different individuals.

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Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

“…Antimicrobial resistance profiles were studied by using the Kirby Bauer technique with the following antibiotics: ampicillin (10 µg), amikacin (30 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), gentamycin (10 µg), kanamycin (30 µg), nalidixic acid (30 µg), streptomycin (10 µg), sulfaprim (trimethoprim-sulfamethoxazole; 25 µg) and tetracycline (30 µg), as described elsewhere [56]. Antimicrobial disks were obtained from Oxoid (United Kingdom).…”