Antibiotic Resistance Profiles of <i>Haemophilus influenzae</i> Isolates from Children in 2016: A Multicenter Study in China

Wang, Hongjiao; Chen, Hua; Yu, Hao; Zhang, Ting; Zhang, Hong; Wang, Shifu; Lin, Aiwei; Cao, Qi; Huang, Wei-Chun; Deng, Huiling; Cao, Shan-Cheng; Chen, Xuejun

doi:10.1155/2019/6456321

Cited by 23 publications

(23 citation statements)

References 27 publications

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“…MDR was present in 41 (6.4%) of the 642 H. in uenzae isolates. The most prevalent resistance phenotype was ampicillin-sulfamethoxazoletrimethoprim-azithromycin resistance, which was in accordance with the previous ndings [15].…”

Section: Discussionsupporting

confidence: 91%

“…In recent years, with the extensive use of antibiotics, the drug resistance of H. in uenzae strains to ampicillin has gradually increased. The ampicillin resistance rate of H. in uenzae strains in China was increased from12% in 2000-2002 [14] to 58.1% in 2016 [15]. In this study, the ampicillin resistance rate was 39.1%, which was higher than that in genital strains (26.4%) but lower than that in respiratory strains (58.4%)in 2015 reported by our study team [15].…”

Section: Discussioncontrasting

confidence: 45%

“…The ampicillin resistance rate of H. in uenzae strains in China was increased from12% in 2000-2002 [14] to 58.1% in 2016 [15]. In this study, the ampicillin resistance rate was 39.1%, which was higher than that in genital strains (26.4%) but lower than that in respiratory strains (58.4%)in 2015 reported by our study team [15]. In different years, the ampicillin resistance of H. in uenzae strains isolated from vulval specimens had gradually increased, from 33.1% in 2016 to 42.8% in 2018.Therefore, it's worth noting of ampicillin resistance of H. in uenzae in clinical management.…”

Section: Discussionmentioning

confidence: 99%

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The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

Zhou

Sun

Chen³

et al. 2020

Preprint

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Background: To determine the prevalence of Haemophilus influenzae vulvovaginitis in prepubertal girls and the antimicrobial resistance of H.influenzae strains isolated from vulval specimens.Methods: Isolates of H.influenzae from vulval swabs of prepubertal girls with vulvovaginitis received at The Children's Hospital, Zhejiang University School of Medicine during 2016-2019 were studied. Vulval specimens were inoculated on Haemophilus selective chocolate agar. Antimicrobial susceptibility tests were performed using the disk diffusion method. A cefinase disk was used to detect β-lactamase. Results: A total of 4142 vulval specimens were received during the 4 years, 649 isolates of H. influenzae were isolated from 642 girls aged 6 months to 13 years, with a median of 5y. There were peaks of isolates from April to July seen in the vulval isolates. In total, the ampicillin resistance rate was 39.1% (250/640); 33.2% strains (211/636) were for β-lactamase-positive isolates, 6.6% strains (42/635) were β-lactamase-negative and ampicillin-resistant (BLNAR) isolates. The resistance rates of H. influenzae isolates to amoxycillin-clavulanic acid, ampicillin-sulbactam, cefuroxime, ceftriaxone, cefotaxime, meropenem, levofloxacin, sulfamethoxazole-trimethoprim, azithromycin, and chloramphenicol were 26.4%, 21.8%, 24.8%, 1.7%, 1.0%, 0.2%, 0%, 47.7%, 10.2%, and 1.1%, respectively. MDR was present in 41 (6.4%) of the 642 H. influenzae isolates, with the most prevalent MDR phenotype of ampicillin-sulfamethoxazole-trimethoprim-azithromycin resistance. Conclusions: H. influenzae is a common cause of vulvovaginitis in prepubertal girls. Laboratories should ensure that they include media appropriate for the isolation of H. influenzae. It’s worth noting of ampicillin resistance of H. influenzae in clinical management.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

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The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

Zhou

Sun

Chen³

et al. 2020

Preprint

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“…Similar findings were also reported by Saikia et al 8 . Very high resistance was also reported in a multicentre study in India 5 , and in China 13 .…”

Section: Introductionsupporting

confidence: 51%

Microbiological Characterization and Antibiotic Susceptibility Pattern of Haemophilus Influenzae Isolates from a Tertiary Care Centre in South India

Nair¹,

Dinesh²,

Karim³

2020

J Pure Appl Microbiol

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Haemophilus are fastidious Gram negative bacilli, which require factor X (hemin), factor V (NAD), or both for their growth. Haemophilus influenzae is the type species, and is considered to be the most pathogenic. They are associated with many invasive infections including meningitis, epiglottitis, pneumonia, and otitis media. Serotype b is most commonly associated with infections. Haemophilus species isolated from patients in a tertiary care centre in South India were studied. Identification, serotyping and biotyping were done and antibiotic susceptibility test was performed. The incidence of H. influenzae infections in our study was 65.3 cases/100,000 persons. Serotype b was the most common (66.67%), followed by non typeable H.influenzae (NTHi) (25%). Most isolates from adults were type b, while all isolates from pediatric population were non typeable. The most common biotype was type II, followed by type I and type III. Three of 24 isolates were β lactamase producers (12.5%). One isolate was β lactamase negative Ampicillin resistant (BLNAR). Resistance to ampicillin was 16.67%. Resistance to cephalosporins and fluoroquinolones was low (4-10%). Co-trimoxazole resistance was found to be very high (75%). All isolates were susceptible to azithromycin, tetracycline, chloramphenicol and meropenem. No isolates of H.influenzae type b were obtained from the paediatric population which may be due to the introduction of Hib vaccine. The increase in resistance to commonly used antibiotics is worrisome, especially penicillins and co-trimoxazole. Use of co-trimoxazole in empirical therapy of upper and lower respiratory tract infections has a high chance of failure in the current scenario.

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“…Second or third-generation cephalosporins or quinolones have become more favorable options for the treatment of H. influenzae infection [7]. However, physicians should more cautiously select antibiotics because of the thriving H. influenzae that is non-susceptible to amoxicillin-clavulanate, fluoroquinolones, second or third-generation cephalosporins, and macrolides [7][8][9][10][11][12]; hence, carbapenems or combination therapies might be empirically considered if appropriate.…”

Section: Introductionmentioning

confidence: 99%

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

Huang

Lai

et al. 2020

BMC Microbiol

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Background: Concern about Haemophilus influenzae infection has been increasing over recent decades. Given the emergence of H. influenzae with severe drug resistance, we assessed the prevalence of as well as risk factors and potential therapies for extensively drug-resistant (XDR) H. influenzae infection in Taiwan. Results: In total, 2091 H. influenzae isolates with disk diffusion-based antibiotic susceptibility testing from 2007 to 2018 were enrolled. H. influenzae strains resistant to ampicillin, chloramphenicol, levofloxacin, and trimethoprimsulfamethoxazole tended to be isolated from patient wards (≧41%), whereas those resistant to amoxicillinclavulanate, cefotaxime, and cefuroxime were more likely to be isolated from intensive care units (approximately 50%). XDR H. influenzae was first identified in 2007, and its incidence did not significantly change thereafter. Overall prevalence of single, multiple, and extensively drug-resistant H. influenzae over 2007-2018 was 21.5% (n = 450), 26.6% (n = 557), and 2.5% (n = 52), respectively. A stepwise logistic regression analysis revealed that blood culture (odds ratio: 4.069, 95% confidence intervals: 1.339-12.365, P = 0.013) was an independent risk factor for XDR H. influenzae infection. No nosocomial transmission of XDR H. influenzae observed. Antibiotic susceptibility testing results demonstrated that cefotaxime was effective against 78.8% (n = 41) of the XDR strains. Conclusions: The presence of XDR H. influenzae strains was identified in Taiwan, and cefotaxime was efficacious against most of these strains.

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Antibiotic Resistance Profiles of Haemophilus influenzae Isolates from Children in 2016: A Multicenter Study in China

Cited by 23 publications

References 27 publications

The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

Microbiological Characterization and Antibiotic Susceptibility Pattern of Haemophilus Influenzae Isolates from a Tertiary Care Centre in South India

Extensively drug-resistant Haemophilus influenzae – emergence, epidemiology, risk factors, and regimen

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