Antibiotic resistance patterns of Pseudomonas spp. isolated from faecal wastes in the environment and contaminated surface water

Camiade, Mathilde; Bodilis, Josselin; Chaftar, Naouel; Riah‐Anglet, Wassila; Gardères, Johan; Buquet, Sylvaine; Ribeiro, Angela Flores; Pawlak, Barbara

doi:10.1093/femsec/fiaa008

Cited by 23 publications

(21 citation statements)

References 60 publications

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“…The contribution of Pseudomonas spp. to the environmental resistome is also large [7], which is also shown in this study.…”

Section: Discussionsupporting

confidence: 88%

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High prevalence of Antibiotic Resistance Bacteria isolated from Municipal Solid Waste Dumpsite, Bahir Dar, Ethiopia

Sitotaw

Ayalew

Girma³

et al. 2021

Preprint

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Background The emergence of antibiotic resistance (ABR) among environmental microbes has been challenging global health. ABR can be transferred to human-associated bacteria aggravating the spread of antimicrobial resistance. Due to poor solid waste disposal practices, municipal solid waste dumpsite (MSWDS) can be a reservoir for antimicrobial resistant microorganisms, such as bacteria. This study aimed to assess the prevalence of antibiotic resistance (ABR) bacteria at Bahir Dar city MSWDS. Methods Soil samples were collected from three randomly selected sites within the dumpsite from November 2020 to May 2021. Bacteria were isolated, identified and tested for ABR using standard procedures. Results In this study, 71 distinct colonies were isolated and identified to ten bacterial genera based on cultural characteristics and biochemical tests. The pooled antibiotic résistance rate was 85.9%. Resistance to the tested antibiotics ranged between 0% for erythromycin and 100% for amoxicillin among the bacterial isolates. High proportions of the isolates were found to be resistant to amoxicillin (100%), vancomycin (87%) and nalidixic acid (73.3%). Substantial proportions of the isolates were also resistant to Streptomycin (54.5%), sulfonamide (50%) and tetracycline (48.5%). On the other hand, high sensitivity rates to erythromycin (90.91%), ciprofloxacin (83.10%), chloramphenicol (77.46%) and gentamicin (63.49%) were recorded. All isolates related to Staphylococcus spp., and most isolates related to Pseudomonas, Escherichia, Klebsiella, Salmonella, Enterococcus, Citrobacter, Shigella and Proteus species were resistant at least to one antibiotic drug. The overall multidrug resistance (MDR) rate was 49.3%, and a high rate of MDR was demonstrated among isolates related to Escherichia spp. (75%), Staphylococcus spp. (68.8%), Pseudomonas spp. (62.5%) and Klebsiella spp. (60%), where each isolate resisted at least 5 antibiotic drugs. Conclusion From the present study, it can be concluded that a high incidence of ABR exists in Bahir Dar city MSWDS. The occurrence of the high level of ABR to commonly used antibiotics in this study demands a proper waste management system, as well as surveillance programs to monitor for antimicrobial resistance determinants in municipal solid wastes. Moreover, detailed studies on the isolates and ABR genes will give a better insight into the prevalence of ABR in the waste dumpsite.

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“…The contribution of Pseudomonas spp. to the environmental resistome is also large [7], which is also shown in this study.…”

Section: Discussionsupporting

confidence: 88%

“…The soil environments, particularly waste dumpsite soils are scarcely investigated for ABR. However, growing interests are shown in the understanding of natural antibiotic resistome [1,7]. Environmental resistome can be a reservoir of resistance genes from which pathogenic bacteria could gain antibiotic resistance genes [7].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of Antibiotic Resistance Bacteria isolated from Municipal Solid Waste Dumpsite, Bahir Dar, Ethiopia

Sitotaw

Ayalew

Girma³

et al. 2021

Preprint

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“…P. aeruginosa and Bacillus sp. reported in the present study are ubiquitous in hospital wastewater discharges, acquiring a wide range of resistance towards antibiotics, heavy metals and other contaminants [55][56][57]. The occurrence of C. davisae in healthcare-associated infections has also been reported by many researchers [13,12].…”

Section: Page 11 / 18supporting

confidence: 77%

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Nath¹,

Sinha²,

Singha³

et al. 2020

Environ Anal Health Toxicol

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Pseudomonas aeruginosa and Bacillus sp. are ubiquitous in hospital wastewater discharges. The infections caused by P. aeruginosa become complicated, as they acquire the ability to resist many classes of antibiotics. Some strains of P. aeruginosa has been reported to survive for months on dry surfaces, and it can persist and grow in contaminated antimicrobial handwash containing triclosan, making it a critical concern for hospital staff [5]. Infections with Bacillus sp. are rare, but they are able to produce spores and grow haemolytically on blood agar plates (β-hemolysis). Gram-positive Bacilli can cause many types of infections and are spread to humans in a variety of ways [6,7]. Some of the frequently encountered species include B.

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“…However, these methodologies are time-consuming, demanding, and/or expensive, particularly in the case of large environmental studies with hundreds or thousands of isolates. Consequently, the use of the 16S rRNA gene to identify Pseudomonas isolates remains relevant despite being prone to misidentifications, as observed frequently in public databases [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Reliable Identification of Environmental Pseudomonas Isolates Using the rpoD Gene

et al. 2020

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The taxonomic affiliation of Pseudomonas isolates is currently assessed by using the 16S rRNA gene, MultiLocus Sequence Analysis (MLSA), or whole genome sequencing. Therefore, microbiologists are facing an arduous choice, either using the universal marker, knowing that these affiliations could be inaccurate, or engaging in more laborious and costly approaches. The rpoD gene, like the 16S rRNA gene, is included in most MLSA procedures and has already been suggested for the rapid identification of certain groups of Pseudomonas. However, a comprehensive overview of the rpoD-based phylogenetic relationships within the Pseudomonas genus is lacking. In this study, we present the rpoD-based phylogeny of 217 type strains of Pseudomonas and defined a cutoff value of 98% nucleotide identity to differentiate strains at the species level. To validate this approach, we sequenced the rpoD of 145 environmental isolates and complemented this analysis with whole genome sequencing. The rpoD sequence allowed us to accurately assign Pseudomonas isolates to 20 known species and represents an excellent first diagnostic tool to identify new Pseudomonas species. Finally, rpoD amplicon sequencing appears as a reliable and low-cost alternative, particularly in the case of large environmental studies with hundreds or thousands of isolates.

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Antibiotic resistance patterns of Pseudomonas spp. isolated from faecal wastes in the environment and contaminated surface water

Cited by 23 publications

References 60 publications

High prevalence of Antibiotic Resistance Bacteria isolated from Municipal Solid Waste Dumpsite, Bahir Dar, Ethiopia

High prevalence of Antibiotic Resistance Bacteria isolated from Municipal Solid Waste Dumpsite, Bahir Dar, Ethiopia

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Reliable Identification of Environmental Pseudomonas Isolates Using the rpoD Gene

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