Antibiotic resistance pattern of Acinetobacter baumannii strains: A retrospective study from Oman

Sannathimmappa, Mohan B; Nambiar, Vinod; Aravindakshan, Rajeev

doi:10.4103/sjmms.sjmms_855_20

Cited by 12 publications

(5 citation statements)

References 27 publications

(35 reference statements)

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“…In the present study, both the VITEK 2 system and E-test strips indicated that the resistance rate of IPM was obviously higher than that of MEM. Similarly, in the North-Batinah region of Oman, a recent study showed that the overall resistance rate of IPM was 72% from 2015 to 2019, which was a bit higher than that of MEM at 70% (Sannathimmappa et al, 2021). Due to the influence of other mechanisms, such as a lack of porins or increased efflux pump activity, the MIC does not entirely reflect the production level of carbapenemases (Jacoby et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

The optimized carbapenem inactivation method for objective and accurate detection of carbapenemase-producing Acinetobacter baumannii

Zhang

Wang

et al. 2023

Front. Microbiol.

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The modified carbapenem inactivation method (mCIM) recommended by the Clinical and Laboratory Standards Institute is not applicable for detecting carbapenemases in Acinetobacter baumannii. Four currently reported phenotypic detection methods, namely, the modified Hodge test, the mCIM, the adjusted mCIM, and the simplified carbapenem inactivation method (sCIM), did not perform well in our 90 clinical A. baumannii isolates. Thus, the minimal inhibitory concentrations (MICs) of carbapenems and the existence and expression of carbapenemase-encoding genes were detected to explain the results. According to the E-test, which was more accurate than the VITEK 2 system, 80.0 and 41.1% were resistant to imipenem (IPM) and meropenem (MEM), respectively, and 14.4 and 53.3% exhibited intermediate resistance, respectively. Five β-lactamase genes were found, of which blaOXA-51-like, blaTEM, and blaOXA-23-like were detected more frequently in 85 non-susceptible strains. The expression of blaOXA-23-like was positively correlated with the MIC values of IPM and MEM. Therefore, an improved approach based on the mCIM, designated the optimized CIM (oCIM), was developed in this study to detect carbapenemases more accurately and reproducibly. The condition was improved by evaluating the factors of A. baumannii inoculum, incubation broth volume, and MEM disk incubation time. Obvious high sensitivity (92.94%) and specificity (100.00%) were obtained using the oCIM, which was cost-effective and reproducible in routine laboratory work.

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Section: Discussionmentioning

confidence: 99%

The optimized carbapenem inactivation method for objective and accurate detection of carbapenemase-producing Acinetobacter baumannii

Zhang

Wang

et al. 2023

Front. Microbiol.

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“…In the case of A. baumannii , the bacteria were 100% resistant to 11 drugs which are given in the table 4, higher resistance rate (70% - 80%) was observed on the ampicillin/sulbactam & trimethoprim/sulfamethoxazole and 30% resistance to colistin. This was very high resistance compared with studies from Oman [55] and Iran [56]. Resistance to the colistin is rapidly emerging among the MDR A. baumannii and K. pneumoniae, which has become a global burden.…”

Section: Discussionmentioning

confidence: 85%

Prevalence of class A ESBL, class B and D carbapenemase encoding genes (CTX-M, TEM, SHV, NDM, IMP, OXA-48) in gram-negative bacterial pathogens isolated from various clinical samples collected from northern region of United Arab Emirates

Ragupathi,

Khamisani,

Sadiq

et al. 2024

Preprint

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Objective The aim of this study was to assess the prevalence of class A ESBL, class B and D carbapenemase encoding genes (blaCTX-M, blaTEM, blaSHV, blaNDM, blaIMP and blaOXA-48) in gram-negative bacterial pathogens isolated from various clinical samples collected from northern region of UAE. Methods A laboratory based experimental study was conducted from October 14, 2021, to June 30, 2022. A total of 3670 various clinical samples (urine, blood, pus, and sputum) were obtained from patients attending the in and out-patient departments of Thumbay University Hospital and various other hospitals of Northern Emirates of UAE, processed for routine bacterial culture examination and determined their antibiotic sensitivity pattern especially ESBL and carbapenem resistance using MicroScan Neg Breakpoint Combo 50 panel. Molecular detection of class A ESBL, class B and D carbapenemase encoding genes (blacTX-M, blaTEM, blaSHV blaNDM, blaIMP & blaOXA-48) were performed on the ESBL and carbapenemase producing gram negative bacteria. Partial genomic sequencing and phylogenetic tree analysis was performed on the most predominant ESBL gene blaCTX-M. Results In total, 1098 bacterial cultures were obtained, of which 833 were gram negative bacteria and among them, 209 (25.1%) were ESBL producers and 40 (4.8%) were both ESBL and carbapenemase producers. In all the 249 bacterial isolates, the ESBL and carbapenemase encoding genes (blaCTX-M, blaTEM, blaSHV, blaNDM & blaIMP) were detected except blaOXA-48. The blaCTX-M (n=72) was the predominantly harbored gene followed by blaTEM (n=56) and blaSHV (n=29), mostly detected in E. coli (n=157), then K. pneumoniae (n=41), P. aeruginosa (n=18) & A. baumannii (n=7). The blaNDM & blaIMP were detected in K. pneumoniae (n=4), A. baumannii (n=3) and P. aeruginosa (n=2) for blaIMP. The gene combinations such as blaCTX-M+TEM (n=15), blaCTX-M+SHV (n=15), blaCTX-M+TEM+SHV (n=6) were co-harbored in 36 E. coli and in the K. pneumoniae, the blaCTX-M+TEM (n=15), blaCTX-M+SHV (n=15), blaTEM+SHV (n=3), blaTEM+NDM (n=1) were detected. The blaCTX-M+TEM, blaCTX-M+SHV & blaCTX-M+TEM+SHV+IMP were detected in one P. aeruginosa and in one A. baumannii, the blaTEM+NDM, blaTEM+IMP & blaCTX-M+TEM+SHV+IMP were detected. Conclusion The highest bacterial culture positivity was detected in the urine samples. 25.1% of the Gram-ve bacteria were only ESBL producers and 4.8% were both the ESBL & carbapenemase producers. 44.5% of ESBL & carbapenemase producing bacteria harbored the blaCTX-M and mostly detected in the E. coli, then K. pneumoniae and P. mirabilis. Multiple gene combinations were detected in 5% P. aeruginosa, 10% A. baumannii and 3.82% E. coli. This finding highlights the importance of molecular detection of ESBL & carbapenemase producing genes to emphasize the monitor and control the development of multi drug resistant Gram-ve bacterial pathogens.

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“…[12,13,14 ] In contrast, a lower percentage, 11.4%, were isolated from ICU, as reported by Kaur et al [15], in other studies, the A. baumannii strains in this study were commonly isolated from clinical samples of patients treated in the medical department compared with the intensive care unit. [16] This could be due to a signicant difference in number of patients who had undergone medical intervention at these two departments. Acinetobacter infection occurs most frequently in critically ill patients particularly those admitted to ICUs as these patients usually require prolonged hospital stays, need repeated invasive procedures, and frequently receive treatment with broad-spectrum antimicrobials.…”

Section: Resultsmentioning

confidence: 99%

Retrospective Study of Extreme Drug Resistant Acinetobacter Baumannii Isolated From Clinical Samples

Pal,

Singh,

Sahni

et al. 2023

PIJR

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Background:Acinetobacter species specifically A. baumannii is increasingly becoming resistant to most antimicrobial agents recommended for treatment. This study aimed to determine the antimicrobial susceptibility pattern of A.baumannii isolated from patients in Gautam Budha Nagar. retrospective study Material and Methods: This was a based on VITEK 2 (BioMérieux) electronic records capturing identification and antimicrobial susceptibility of Acinetobacter isolates from patient samples analyzed between Jan 2021 and Mar 2023 at GIMS Hospital, Gautam Budha Nagar. A total of 450 Results: A.baumannii were recored. 73.1% of the isolates tested were extreme drug resistant (XDR). Among the 450 isolates, 153 (34%) were from sputum and 176(39%) from the critical care unit. A. baumannii was the most frequently isolated species with high resistant high level of resistance to cephalosporins (91%), ciprofloxacin (96%), and meropenem (76%). It showed susceptibility to colistin only. Conclusion: A. baumannii is resistant to commonly administered antibiotics. There is need for continuous antimicrobial resistance surveillance especially in health care facilities and strengthening of antibiotic stewardship programmes which will contribute to enhancement of infection control policies.

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Antibiotic resistance pattern of Acinetobacter baumannii strains: A retrospective study from Oman

Cited by 12 publications

References 27 publications

The optimized carbapenem inactivation method for objective and accurate detection of carbapenemase-producing Acinetobacter baumannii

The optimized carbapenem inactivation method for objective and accurate detection of carbapenemase-producing Acinetobacter baumannii

Prevalence of class A ESBL, class B and D carbapenemase encoding genes (CTX-M, TEM, SHV, NDM, IMP, OXA-48) in gram-negative bacterial pathogens isolated from various clinical samples collected from northern region of United Arab Emirates

Retrospective Study of Extreme Drug Resistant Acinetobacter Baumannii Isolated From Clinical Samples

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