Antibiotic resistance of Vibrio parahaemolyticus isolated from coastal seawater and sediment in Malaysia

Drais, Ashraf Abbas; Usup, Gires; Ali, Ahmad

doi:10.1063/1.4966722

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The occurrence of multi-antibiotic resistant bacteria in seafood and aquatic environments is a major concern and can pose a great risk to human health (Elmahdi et al, 2016). This warrants a necessity for stringent supervision, restricted use of antibiotics and frequent surveillance of V. parahaemolyticus strains for resistance to antibiotics (Drais et al, 2016;Park et al, 2018). Majority of the strains in our study showed resistance to ampicillin and vancomycin.…”

Section: Distribution Of Crispr In V Parahaemolyticus Isolatesmentioning

confidence: 68%

Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

Baliga¹,

Shekar²,

Ahamed³

et al. 2019

Indian J. Fish.

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Vibrio parahaemolyticus is a pathogen native to the aquatic environment. In this study, 46 environmental V. parahaemolyticus isolates were subjected to a correlational analysis to find the association between their antimicrobial susceptibility pattern, prevalence of CRISPR-Cas system and thermostable direct hemolysin (tdh) gene. Antibiotic resistance profiling against eleven antibiotics revealed the isolates to be multidrug resistant. Isolates exhibited highest resistance to vancomycin (97.8%) followed by ampicillin (91.3%), cefotaxime (69.6%), ceftazidime/clavulanic acid (54.4%), ceftazidime (45.7%) and gentamicin (39.1%). CRISPR loci and tdh gene were detected in 47.83 and 58.7% of strains respectively. No significant correlation was observed between antibiotic resistance to presence of CRISPR, except in the case of gentamicin wherein, a negative correlation was seen (r=-0.272, p<0.10). Similarly, tdh did not correlate to antibiotic resistance. Seventeen strains in this study harboured the CRISPR loci as well as tdh gene, the association of which was found to be statistically significant.

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Section: Distribution Of Crispr In V Parahaemolyticus Isolatesmentioning

confidence: 68%

Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

Baliga¹,

Shekar²,

Ahamed³

et al. 2019

Indian J. Fish.

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“…Many classes of antimicrobial agents were previously detected in environmental samples such as sewage, effluents, surface water, and wastewater treatment plants (Lundborg and Tamhankar, 2017). For example, the previous studies from the U.S., Malaysia, Brazil, and Jordan reported that pathogenic V. parahaemolyticus was isolated from estuary and coastal water contained high prevalence of AMR (Baker-Austin et al, 2008;Alaboudi et al, 2016;Drais et al, 2016;Menezes et al, 2017). These findings indicated that animal effluents and wastewater come from various sources may play an important role in the dissemination of AMR in the environment that can impact on oysters and estuarine water quality.…”

Section: Logistic Regression Model For the Association Between Antimi...mentioning

confidence: 99%

Detection of antimicrobial resistance determinants in vibrio parahaemolyticus isolated from cultivated oysters and estuarine waters

Khant

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In this study, we aimed to detect antimicrobial resistance determinants and virulence factor genes in V. parahaemolyticus isolates from cultivated oysters and estuarine waters in southern Thailand. A total of 594 V. parahaemolyticus isolates were collected from pooled oysters (n=361) and estuarine waters (n=233). The samples were collected monthly between March 2016 and February 2017 from Thap Put district, Phang Nga province in southern Thailand. Confirmation of V. parahaemolyticus was carried out by PCR assay detecting species-specific (tlh) gene and the presence of virulence genes (tdh and trh). Antimicrobial susceptibility test was performed in eight antimicrobials, and the occurrence of resistance genes was investigated. All isolates were detected for the presence of SXT elements and class 1, 2, and 3 integrons. The results showed that all presumptive V. parahaemolyticus isolates (n=594) were positive to species-specific (tlh) gene. Four isolates (0.7%) from pooled oysters (n=2) and estuarine waters (n=2) were positive to tdh. None of the trh-positive isolates were observed. In this study, 34% of the isolates were resistant to at least one antimicrobial agent and 5.1% of the isolates were multidrug-resistant (MDR). Most of the isolates were resistant to erythromycin (54.2%), followed by sulfamethoxazole (34.7%), trimethoprim (28.0%), and ampicillin (11.1%), respectively. Only 0.8% and 0.5% of the isolates were resistant to streptomycin and tetracycline, respectively, and chloramphenicol and ciprofloxacin resistance were not observed in all isolates. Among the tested antimicrobials, the prevalence of tetracycline resistance in estuarine waters was significantly higher than oyster (P < 0.05). The most frequent resistance pattern was ERY (21.0%) and the most common MDR phenotype was ERY-SUL-AMP (1.9%). The most commonly found AMR genes were qnr (77.8%) and strB (27.4%), followed by tetA (22.1%), dfr18 (19.5%), ermB (15.2%), and sul2 (14.8%), respectively. However, dfrA1 (7.4%) and blaTEM (0.8%) genes were rarely found AMR genes in this study. The occurrence of qnr and dfr18 genes were significantly different between oysters and estuarine water samples (P < 0.05). None of the isolates were possessed SXT integrase gene (intSXT) and class 1, 2, and 3 integrons. Our results highlighted the need for improving good sanitary practices and consumption of adequate cooked oysters to promote seafood safety for consumption and to reduce the risk of seafood-borne illnesses and antimicrobial resistance infection.

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Antibiotic resistance of Vibrio parahaemolyticus isolated from coastal seawater and sediment in Malaysia

Cited by 2 publications

References 13 publications

Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

Detection of antimicrobial resistance determinants in vibrio parahaemolyticus isolated from cultivated oysters and estuarine waters

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