Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil

Abstract

Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14-0.29), indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.

High prevalence of antimicrobial resistance in Escherichia coli , Salmonella spp. and Staphylococcus aureus isolated from fish samples in India

“…Our study reveals high proportion of resistance towards amikacin, streptomycin, trimethoprim and azithromycin common in three genus. Regardless of the type of bacteria, a study published in India and Brazil also reported high proportion of amikacin (~60%) (Manjusha & Sarita, ; Melo et al, ). Along with this, others have reported approximately 10%–29% of resistance against amikacin in aquaculture environment (Resende et al, ).…”

“…Our study reveals high proportion of resistance towards amikacin, streptomycin, trimethoprim and azithromycin common in three genus. Regardless of the type of bacteria, a study published in India and Brazil also reported high proportion of amikacin (~60%) (Manjusha & Sarita, 2011;Melo et al, 2011).…”

Molecular Identification of AHPND Positive Vibrio Parahaemolyticus causing an outbreak in South-West Shrimp Farming Regions of Bangladesh

“…No isolates were resistant to Kanamycin, Gentamycin, Nalidixic acid and Nitrofurantoin. De Melo et al (2011) reported that in antibiotic susceptibility test five strains (50%) presented multiple antibiotic resistant to ampicillin (90%) and Amikacin (60%), while two strains (20%) displayed intermediate-level of resistance to Amikacin. Xu et al (2016) reported the antimicrobial resistance patterns of 145 isolates of V. parahaemolyticus to 12 antimicrobial agents revealed that most of the isolates resistant to streptomycin, with resistance and intermediate rates of 86.2 % and 11.7 %, respectively.…”

First Detection of AmpC β-Lactamase bla CMY-2 on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin

“…The increasing prevalence of antimicrobial resistance in V. parahaemolyticus, presumably due to the extensive use of antimicrobials in clinical treatment and aquaculture systems, was recently reported (6)(7)(8)(9). To date, resistance to ampicillin, streptomycin, kanamycin, tetracycline, chloramphenicol, and ciprofloxacin has been reported (9).…”