Antibiotic resistance of subgingival species during and after antibiotic therapy

Feres, Magda; Haffajee, A. D.; Allard, K.; Som, S.; Goodson, J. Max; Socransky, S. S.

doi:10.1034/j.1600-051x.2002.290809.x

Cited by 119 publications

(162 citation statements)

References 46 publications

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“…Modified tryptic soy agar [19] ("real" periodontopathic bacteria) and trypic soy agar were used as cultivation media. A. actinomycetemcomitans strains and Streptococcus constellatus ATCC 27823 were always incubated with 5% CO 2 , the other "real" periodontopathogens were incubated anaerobically and all potentially superinfecting species within normal atmospheric conditions, each at 37°C.…”

Section: Microorganismsmentioning

confidence: 99%

Efficacy of taurolidine against periodontopathic species—an in vitro study

et al. 2011

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The antimicrobial effect of taurolidine was tested against periodontopathic species in comparison to chlorhexidine digluconate in the presence or absence of serum. Minimal inhibitory concentrations (MIC), microbiocidal concentrations (MBC), as well as killing were determined against 32 different microbial strains including 3 Porphyromonas gingivalis, 3 Aggregatibacter actinomycetemcomitans, and 15 potentially superinfecting species with and without 25% v/v human serum. The MIC 50 of taurolidine against the tested microbial strains was 0.025% and the MIC 90 0.05%. The respective values for the MBCs were 0.05% and 0.1%. Addition of 25% serum (heat-inactivated) did not change the MIC and MBC values of taurolidine. In contrast, MICs and MBCs of chlorhexidine (CHX) increased by two steps after addition of serum. Taurolidine killed microorganisms in a concentration and timedependent manner, the killing rate of 1.6% taurolidine was 99.08%±2.27% in mean after 2 h. Again, killing activity of taurolidine was not affected if serum was added, whereas addition of inactivated serum clearly reduced the killing rate of all selected bacterial strains by CHX. Therefore, taurolidine possesses antimicrobial properties which are not reduced in the presence of serum as a main component in gingival crevicular fluid and wound fluid. Taurolidine may have potential as an antimicrobial agent in non-surgical and surgical periodontal treatment.

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Section: Microorganismsmentioning

confidence: 99%

Efficacy of taurolidine against periodontopathic species—an in vitro study

et al. 2011

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“…As a result, there is a pronounced interest in the development of alternative antimicrobial concepts [7][8][9] .…”

Section: ⅰ Introductionmentioning

confidence: 99%

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

Jung¹,

Park²,

Lee

et al. 2012

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. The aim of this study was to evaluate the effect of PDT on Streptococcus mutans in planktonic conditions, previously treated with different photosensitive concentrations of erythrosine, using halogen and LED curing unit as a light source. And we compared the effects of PDT on six strains of S. mutans isolated from oral cavity and reference strain.As a result, S. mutans was susceptible to the combination of hand held photopolymerizer (HHP) and erythrosine. The higher concentration of erythrosine in the presence of light irradiation induced greater effects in reduction of viability of S. mutans. Isolated S. mutans showed a significant reduction in bacterial counts of the groups submitted to PDT compared to the control groups. And they appeared to be similar or slightly lower antimicrobial effect compared with reference strain. However, the difference was not significant (p < 0.05).In conclusion, PDT using erythrosine as a photosensitizing agent and HHP as a light source could be an efficient option for diseases caused by S. mutans.

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“…6 Despite the many complications caused by periodontitis, no treatment protocol is known to be able to fully control this disease. Currently, a combination of mechanical treatments and systemic antibiotics are used as treatment 7 ; however, periodontal pathogens are not completely removed by the mechanical removal of the biofilm; and there are problems associated with the use of antibiotics, 9 and eventual complications related to antibiotics such as allergic reactions, gastrointestinal problems and bacterial resistance. 10 Therefore, researchers are trying to find alternative methods for treatment of periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

et al. 2016

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IntroductionPeriodontal diseases are referred to as inflammatory processes in periodontal tissues in response to the aggregation of bacteria on the teeth. The aggregation rarely leads to evident infection, but the inflammatory response caused in the gingival tissue is responsible for the gradual loss of dental collagen's attachment to the alveolar bone. Left overlooked, this phenomenon might end in increased dental mobility and eventually edentulism.1 Production of destructive metabolites by gram negative and positive bacteria of the microbial plaque in the oral cavity causes gingivitis, which can play a key role in the progression of inflammation to periodontal diseases. 2Aggregatibacter actinomycetemcomitans is an immobile microaerophilic, facultative anaerobic, gram negative coccoid rod, 3 strongly associated with pathogenesis of periodontal diseases, particularly aggressive generalized periodontitis.4 Some of this bacterium's membranous proteins play an important role in attachment to and penetration in tissue cells.5 Other virulence factors secreted by this bacterium include an agent that inhibits the reproduction of fibroblasts and a leukotoxin that kills the leukocytes. 6 Despite the many complications caused by periodontitis, no treatment protocol is known to be able to fully control this disease. Currently, a combination of mechanical treatments and systemic antibiotics are used as treatment 7 ; however, periodontal pathogens are not completely removed by the mechanical removal of the biofilm; and there are problems associated with the use of antibiotics, Abstract Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (CHX), curcumin and light-emitting diode (LED) laser, on this bacterium. Methods: Antimicrobial activity of curcumin (5 mg/ml), CHX (2%), LED (120 J/cm 2 ) and LED + curcumin (120 J/cm 2 + 2.5 mg/ml) against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance (ANOVA) and Tukey's HSD tests served for statistical analysis. Results: Regarding the minimum inhibitory concentration (MIC), CHX had a significantly lower MIC than curcumin (P < 0.05). Sorted out by bacterial growth from lowest to highest, were CHX, LED + curcumin, curcumin, and LED groups. All the differences were found to be statistically significant (P < 0.05) except for the LED group. Conclusion: We conclude that curcumin is an effective substance in preventing the growth of A. actinomycetemcomitans, whose impact is reinforced when used simultaneously with photodynamic therapy (PDT).

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Antibiotic resistance of subgingival species during and after antibiotic therapy

Cited by 119 publications

References 46 publications

Efficacy of taurolidine against periodontopathic species—an in vitro study

Efficacy of taurolidine against periodontopathic species—an in vitro study

The Effect of Photodynamic Therapy on the Viability of Streptococcus Mutans Isolated From Oral Cavity

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

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