Antibiotic Resistance of
            <i>Campylobacter</i>
            Species in a Pediatric Cohort Study

Schiaffino, Francesca; Colston, Josh M.; Olórtegui, Maribel Paredes; François, Ruthly; Pisanic, Nora; Burga, Rosa; Peñataro-Yori, Pablo; Kosek, Margaret

doi:10.1128/aac.01911-18

Cited by 48 publications

(46 citation statements)

References 71 publications

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“…When considering the phenotypic resistance, MDR was noted in 55.8% of the isolates. Simultaneously, data presented in different geographical regions showed high and extremely high prevalence of MDR among Campylobacter isolates (Schiaffino et al, 2019). Further analysis revealed that all isolates assigned to ST-257, ST-429, ST-464, ST-572, and ST-824 were associated with multiresistance.…”

Section: Discussionmentioning

confidence: 79%

“…Interestingly the combination of penicillintype antibiotic (AMP, amoxicillin/clavulanic) was highly effective against Campylobacter (resistance exhibited by 2 out 43 isolates, CI 95%: 1.3-15.5%).No resistance to amoxicillin-clavulanic acid was detected by Post et al (2017) in Belgium and Wallace et al (2019) in Australia in clinical isolates. Also, the study conducted by Schiaffino et al (2019) provides compelling evidence to propose amoxicillin/clavulanic acid as an effective treatment for campylobacteriosis.…”

Section: Discussionmentioning

confidence: 99%

“…Emonet et al (2017) suggest that ceftriaxone is often prescribed empirically for patients hospitalized with abdominal pain and fever. However, in many studies this antimicrobial agent is not recommended for treatment of campylobacterisosis (Emonet et al, 2017;Schiaffino et al, 2019). Similarly, trimethoprim/sulfamethoxazole is not recommended to be used in routine treatment of campylobacteriosis.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Resistance and Virulence-Associated Markers in Campylobacter Strains From Diarrheic and Non-diarrheic Humans in Poland

et al. 2020

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Campylobacteriosis is one of the most common causes of bacterial gastroenteritis. However, the clinical course of the illness varies in symptoms and severity. The aim of this study was to characterize Campylobacter jejuni (34 isolates) and C. coli (9 isolates) from persons with diarrheal and non-diarrheal stools at the time of examination and fecal sampling, in Poland by using whole-genome sequencing (WGS). Multilocus sequence typing (MLST) analysis revealed a high diversity with a total of 20 sequence types (STs) among 26 Campylobacter isolates from diarrheic and 13 STs among 17 isolates from non-diarrheic persons. ST-50 and ST-257 were most common in both groups. The phenotypic resistance rate was 74.4% for ciprofloxacin, 67.4% for sulfamethoxazole/trimethoprim, 58.1% for amoxicillin, 48.8% for tetracycline, and 46.5% for ceftriaxone. Only single isolates were resistant to erythromycin, gentamicin, and amoxicillin/clavulanic acid. Overall genotypic resistance toward amoxicillin, fluoroquinolones, tetracyclines, and aminoglycosides was predicted to occur in 93.1, 67.4, 48.8, and 11.6% of the isolates, respectively. None of the isolates showed the presence of the erm(B) gene or mutation in 23S rRNA. Neither was variation found in the important target region in L4 and L22 ribosomal proteins. In regard to the CmeABC efflux pump, a set of variable mutations affecting the regulatory region was noted. All Campylobacter isolates possessed genes associated with adhesion (cadF, jlpA, porA, and pebA) and invasion (ciaB, pldA, and flaC). The type IV secretion system (T4SS) was found in isolates from both diarrheic (15.4%, CI 95%: 6.1-33.5%) and non-diarrheic (23.5%, CI 95%: 9.6-47.3%) persons. The rates of the presence of cytolethal distending toxin cdtABC gene cluster and type VI secretion system (T6SS) were higher in Campylobacter isolates obtained from persons with diarrhea (96.2%, CI 95%: 81.7-99.3% and 26.9%, CI 95%: 13.7-46.1%) compared to isolates from

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Resistance and Virulence-Associated Markers in Campylobacter Strains From Diarrheic and Non-diarrheic Humans in Poland

et al. 2020

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“…Samples collected as part of a cohort study from Iquitos, in the Peruvian Amazon, between 2002 and 2006. In this age-stratified sample set of 442 children aged 0-5 years [7,[13][14][15]41,42], children were visited 3 times weekly to form a continuous symptom history of childhood illnesses. Stool samples were collected quarterly from all children and in cases in which diarrhea was detected (92.3% of episodes detected by surveillance had a sample collected;…”

Section: Sampling and Cohort Informationmentioning

confidence: 99%

Genomic epidemiology ofCampylobacter jejuniassociated with asymptomatic pediatric infection in the Peruvian Amazon

Pascoe

Schiaffino

Murray

et al. 2020

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Campylobacter is the leading bacterial cause of gastroenteritis worldwide and its incidence is especially high in low- and middle-income countries (LMIC). Disease epidemiology in LMICs is different compared to high income countries like the USA or in Europe. Children in LMICs commonly have repeated and chronic infections even in the absence of symptoms, which can lead to deficits in early childhood development. In this study, we sequenced and characterized C. jejuni (n=62) from a longitudinal cohort study of children under the age of 5 with and without diarrheal symptoms, and contextualized them within a global C. jejuni genome collection. Epidemiological differences in disease presentation were reflected in the genomes, specifically by the absence of some of the most common global disease-causing lineages. As in many other countries, poultry-associated strains were a major source of human infection but almost half of local disease cases (15 of 31) were attributable to genotypes that are rare outside of Peru. Asymptomatic infection was not limited to a single (or few) human adapted lineages but resulted from phylogenetically divergent strains suggesting an important role for host factors in the cryptic epidemiology of campylobacteriosis in LMICs.

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“…However, in severe cases, antibiotics such as ciprofloxacin, tetracycline and erythromycin can be prescribed [6]. However, the efficiency of antibiotics against Campylobacter infections is decreasing due to an increase in antibiotic resistance [7]. The use of antibiotics in poultry production may contribute to the emergence of resistant strains in human through the food chain [8].…”

Section: Introductionmentioning

confidence: 99%

Reuterin Demonstrates Potent Antimicrobial Activity Against a Broad Panel of Human and Poultry Meat Campylobacter spp. Isolates

et al. 2020

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Reuterin is a broad-spectrum antimicrobial system produced by specific strains of Lactobacillus reuteri during anaerobic metabolism of glycerol. Acrolein is the main component responsible for its antimicrobial activity. Here, the sensitivity of Campylobacter jejuni (n = 51) and Campylobacter coli (n = 20) isolates from chicken meat and human stool samples to reuterin was investigated. The minimum inhibitory concentration (MIC) of C. jejuni and C. coli strains was measured between 1.5 and 3.0 µM of acrolein, below the MIC of the sensitive indicator strain Escherichia coli K12 (16.5 µM acrolein). The interaction of C. jejuni N16-1419 and the reuterin-producing L. reuteri PTA5_F13 was studied during 24 h co-cultures with or without glycerol. A high C. jejuni growth was observed in cultures without glycerol. In contrast, C. jejuni growth decreased from 7.3 ± 0.1 log CFU/mL to below detection limit (1 log CFU/mL) during co-cultures added with 28 mM glycerol. This bactericidal effect could be attributed to in situ reuterin production. The low MIC observed and the high sensitivity towards in situ produced reuterin suggests L. reuteri combined with glycerol, as a possible intervention option to reduce Campylobacter in the food chain.

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Antibiotic Resistance of Campylobacter Species in a Pediatric Cohort Study

Abstract: The objective of this study was to determine the phenotypic patterns of antibiotic resistance and the epidemiology of drug-resistant Campylobacter spp. from a low-resource setting.

Cited by 48 publications

References 71 publications

Antimicrobial Resistance and Virulence-Associated Markers in Campylobacter Strains From Diarrheic and Non-diarrheic Humans in Poland

Antimicrobial Resistance and Virulence-Associated Markers in Campylobacter Strains From Diarrheic and Non-diarrheic Humans in Poland

Genomic epidemiology ofCampylobacter jejuniassociated with asymptomatic pediatric infection in the Peruvian Amazon

Reuterin Demonstrates Potent Antimicrobial Activity Against a Broad Panel of Human and Poultry Meat Campylobacter spp. Isolates

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