Antibiotic resistance in Escherichia coli isolates from roof-harvested rainwater tanks and urban pigeon faeces as the likely source of contamination

Chidamba, Lizyben; Korsten, Lisé

doi:10.1007/s10661-015-4636-x

Cited by 18 publications

(9 citation statements)

References 46 publications

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“…Resistance or reduced susceptibility was observed in the case of AMC (20.00% of strains), AMP (26.67%), CAZ (5.00%), CTX (13.33%), FOX (6.67%), ATM (15.00%), NA (5.00%), CIP (1.67%), C (5.00%), CN (11.67%), AK (11.67%), STR (55.00%), TOB (20.00%) and TE (6.67%). High levels of resistance to such antimicrobials have also been reported in E. coli from domestic birds [33,34,35,36,37,38,39] and from various kinds of wild birds and mammals living in close proximity to humans, including pigeons [14,40,41,42,43,44,45,46,47,48,49,50,51,52,53].…”

Section: Resultsmentioning

confidence: 99%

Microbial Load and Antibiotic Resistance Patterns of Escherichia coli and Enterococcus faecalis Isolates from the Meat of Wild and Domestic Pigeons

Cordero

Alonso‐Calleja

García

et al. 2019

Foods

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An expansion in the consumption of pigeon meat has occurred in recent years. However, little is known about microbial load and antibiotic resistance of this foodstuff. The hygiene status and the antibiotic resistance patterns (disc diffusion; Clinical and Laboratory Standards Institute, CLSI) of Escherichia coli and Enterococcus faecalis isolates from wild and domestic pigeon carcasses were investigated. Average microbial loads (log10 cfu/cm2) ranged from 1.40 ± 1.17 (fecal coliforms) to 3.68 ± 1.40 (psychrotrophs). The highest (p < 0.05) microbial loads were observed in domestic pigeons. No substantial differences were found between isolates from domestic and wild pigeons with regard to the prevalence of antibiotic resistance. Of the E. coli strains, 20.00% were susceptible, 25.00% showed resistance or reduced susceptibility to one antimicrobial and 55.00% were multi-resistant. Among the E. faecalis isolates, 2.22% were susceptible and 97.78% were multi-resistant. The greatest prevalence of resistance or reduced susceptibility among E. coli was observed for amoxicillin-clavulanic acid (20.00% strains), ampicillin (26.67%), streptomycin (55.00%) and tobramycin (20.00%). The prevalence of resistance or reduced susceptibility among E. faecalis ranged from 31.11% (trimethoprim/sulfamethoxazole) to 97.78% (erythromycin). Meat from pigeons is a major reservoir of antibiotic-resistant bacteria. The need for the correct handling of this foodstuff in order to reduce risks to consumers is underlined.

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Section: Resultsmentioning

confidence: 99%

Microbial Load and Antibiotic Resistance Patterns of Escherichia coli and Enterococcus faecalis Isolates from the Meat of Wild and Domestic Pigeons

Cordero

Alonso‐Calleja

García

et al. 2019

Foods

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“…Bird faecal droppings may negatively impact roof-harvested rainwater quality due to the presence of zoonotic pathogens [ 32 ]. A study conducted in South Africa investigated antibiotic resistance in E. coli isolates from roof-harvested rainwater tanks and urban pigeon faeces as the likely source of contamination and concluded that urban pigeons, the most likely source of HRW contamination, are also reservoirs of multiple antibiotic-resistant bacteria [ 33 ]. The findings of the South African study on bird faeces and antibiotic-resistant E. coli have a similar conclusion to our study where bird faecal matter was suspected to contribute to the contamination of HRW.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa

Malema

Abia

Tandlich

et al. 2018

IJERPH

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Although many developing countries use harvested rainwater (HRW) for drinking and other household purposes, its quality is seldom monitored. Continuous assessment of the microbial quality of HRW would ensure the safety of users of such water. The current study investigated the prevalence of pathogenic Escherichia coli strains and their antimicrobial resistance patterns in HRW tanks in the Eastern Cape, South Africa. Rainwater samples were collected weekly between June and September 2016 from 11 tanks in various areas of the province. Enumeration of E. coli was performed using the Colilert®18/Quanti-Tray® 2000 method. E. coli isolates were obtained and screened for their virulence potentials using polymerase chain reaction (PCR), and subsequently tested for antibiotic resistance using the disc-diffusion method against 11 antibiotics. The pathotype most detected was the neonatal meningitis E. coli (NMEC) (ibeA 28%) while pathotype enteroaggregative E. coli (EAEC) was not detected. The highest resistance of the E. coli isolates was observed against Cephalothin (76%). All tested pathotypes were susceptible to Gentamicin, and 52% demonstrated multiple-antibiotic resistance (MAR). The results of the current study are of public health concern since the use of untreated harvested rainwater for potable purposes may pose a risk of transmission of pathogenic and antimicrobial-resistant E. coli.

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“…In a low-income setting in Nicaragua, Hasan et al reported the existence of ESBLproducing E. coli in about 13% poultry pigeons compared to 8% in other domestic and wild birds, and 27% in healthy humans (Hasan et al, 2016). Interestingly, another study from South Africa addressed the risk of AMR E. coli spread due to pigeon fecal samples contamination of the roof-harvested rainwater (Chidamba & Korsten, 2015). In particular, they reported resistance to ampicillin (22.7.9%), gentamicin (23.6%), amikacin (24%), tetracycline (17.4) and amoxicillin (16.9%) (Chidamba & Korsten, 2015).…”

Section: Amr In Commensal E Coli From Pigeonsmentioning

confidence: 99%

“…Interestingly, another study from South Africa addressed the risk of AMR E. coli spread due to pigeon fecal samples contamination of the roof-harvested rainwater (Chidamba & Korsten, 2015). In particular, they reported resistance to ampicillin (22.7.9%), gentamicin (23.6%), amikacin (24%), tetracycline (17.4) and amoxicillin (16.9%) (Chidamba & Korsten, 2015). In another study from Iran that evaluated AMR of commensal E. coli isolated from fecal samples from pigeons, high resistance to tetracycline (88·4%) and doxycycline (74·4%) was reported (Askari Badouei, Zahraei Salehi, Koochakzadeh, Kalantari, & Tabatabaei, 2014).…”

Section: Amr In Commensal E Coli From Pigeonsmentioning

confidence: 99%

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

Alhababi

Eltai

Nasrallah

et al. 2020

Microbial Drug Resistance

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Background: The dissemination of antimicrobial resistance (AMR) bacteria is associated with the inappropriate use of antibiotics in both humans and animals. In particular, the use of antibiotics for prophylactic and growth promotion purposes in foodproducing animals, in addition to animal-group medication for prolonged times, have rendered many of the antibiotics ineffective, returning us to the old times when a simple infection could be deadly. Objectives: This study aims at evaluating the incidence of AMR in Qatar under the one health approach; i.e., human-animal-environmental interface.Specifically, we aim to characterize the phenotypic and genotypic AMR pattern of 18 clinically relevant antibiotics in major food-producing animals. Ultimately, we aim to compare our findings to the data released by the monitoring programs in Hamad Medical Corporation (HMC) along with previously published reports from Qatar. Methods: Animal fecal samples from camels, cattle, and pigeons (100 samples from each species) were collected from slaughterhouses and farms distributed in different locations in Qatar. One gram of fecal sample was vigorously homogenized with 3 ml sterile Phosphate Buffered Saline (PBS), the suspension was streaked directly onto chromogenic Tryptone Bile Xglucuronide agar for E. coli detection. Single typical E. coli colonies (blue-green) were randomly selected and streaked onto MacConkey agar plates and then recovered on iv nutrient agar plates for lactose fermentation testing by indole spot. The antibiotic susceptibility test was conducted using a Kirby-Bauer disk diffusion assay on Mueller-Hinton agar plates. Results: A total recovery rate of 88.7% (n=266) achieved from all samples. Overall, E. coli isolates had resistance to ten antibiotics in pigeon group, eight antibiotics in cattle group and only five antibiotics in camel group. Resistance to at least one antibiotic was recorded in 63 E. coli isolates (70.7%) from pigeons, 32 isolates (37.2%) from cattle and only in 19 isolates (20.8%) from camels. Multi-drug resistant (MDR) was highest in isolates from pigeons reaching 50% (n=44), followed by isolates from cattle (7%) and camels (2.2%). Highest resistance rate was observed against tetracycline, reaching a frequency of 64%, 27.9% and 15% of isolates from pigeons, cattle and camels, respectively (p <0.0001). Moreover, trimethoprim/sulfamethoxazole (an antibiotic used to treat a variety of bacterial infections) resistance was present in 22.2% (n=59) of all E. coli isolates, with the highest prevalence in pigeons (49.4%), followed by 11.6% in cattle and 5% in camels. Interestingly, one E. coli isolate from pigeon showed resistance to colistin, a drug of last resort in human medicine against gram-negative bacterial infection.Conclusions: We previously reported high multi-drug resistance of commensal E. coli in chickens, with significant resistance to colistin. We observed lower AMR profile in ruminants, presumably due to the different antibiotics used in the two animal industries.

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Antibiotic resistance in Escherichia coli isolates from roof-harvested rainwater tanks and urban pigeon faeces as the likely source of contamination

Cited by 18 publications

References 46 publications

Microbial Load and Antibiotic Resistance Patterns of Escherichia coli and Enterococcus faecalis Isolates from the Meat of Wild and Domestic Pigeons

Microbial Load and Antibiotic Resistance Patterns of Escherichia coli and Enterococcus faecalis Isolates from the Meat of Wild and Domestic Pigeons

Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa

Antimicrobial Resistance of Commensal Escherichia coli Isolated from Food Animals in Qatar

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