Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from poultry in the South-East Queensland region

Miflin, J.K.; Templeton, Jillian; Blackall, P. J.

doi:10.1093/jac/dkm024

Cited by 57 publications

(62 citation statements)

References 15 publications

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“…Sensitivity to chloramphenicol by all Campylobacter isolates was also observed by Sáenz et al (2000) and Guévremont et al (2006) in pigs, whereas Bardon et al (2009) andMiflin et al (2007) found similar resistance patterns for Campylobacter spp. obtained from humans and chickens, respectively.…”

Section: Discussionsupporting

confidence: 72%

“…On the other hand, Miflin et al (2007) found sensitivity to ciprofloxacin for all the 152 strains of Campylobacter spp. isolated from chicken in Australia, whereas Inglis et al (2005) confirmed ciprofloxacin resistance in only 0.3% of isolates from cattle in Canada.…”

Section: Discussionmentioning

confidence: 94%

“…strains isolated from animal products has been reported in different countries such as Italy (Pezzotti et al, 2003), Denmark (Alban, Nielsen, & Dahl, 2008), Korea (Shin & Lee, 2007), Sweden (Rönner et al, 2004), the United States (Olah, Doetkottb, Fakhra, & Loguea, 2006), the United Kingdom (Little, Richardson, Owen, Pinna, & Threlfall, 2008), Austria (Mayrhofer et al, 2004), Australia (Miflin, Templeton, & Blackall, 2007), Czech Republic (Bardon et al, 2009) and Canada (Guévremont et al, 2006;Inglis et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Biasi¹,

Macedo²,

Malaquias³

et al. 2011

Food Control

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Biasi¹,

Macedo²,

Malaquias³

et al. 2011

Food Control

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“…The flagellin gene has been a well-accepted marker for Campylobacter genotyping for over 2 decades (4,16,18,19,22,25,32). In this study we have developed a genotyping method based on flaA interrogation using HRM analysis.…”

Section: Discussionmentioning

confidence: 99%

Campylobacter jejuni and Campylobacter coli Genotyping by High-Resolution Melting Analysis of a flaA Fragment

Merchant-Patel

Blackall²,

Templeton³

et al. 2010

Appl Environ Microbiol

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The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It was found that the PCR primers commonly used to amplify the flaA short variable repeat (SVR) yielded a mixed PCR product unsuitable for HRM analysis. However, a PCR primer set composed of the upstream primer used to amplify the fragment used for flaA restriction fragment length polymorphism (RFLP) analysis and the downstream primer used for flaA SVR amplification generated a very pure PCR product, and this primer set was used for the remainder of the study. Eighty-seven C. jejuni and 15 C. coli isolates were analyzed by flaA HRM and also partial flaA sequencing. There were 47 flaA sequence variants, and all were resolved by HRM analysis. The isolates used had previously also been genotyped using single-nucleotide polymorphisms (SNPs), binary markers, CRISPR HRM, and flaA RFLP. flaA HRM analysis provided resolving power multiplicative to the SNPs, binary markers, and CRISPR HRM and largely concordant with the flaA RFLP. It was concluded that HRM analysis is a promising approach to genotyping based on highly variable genes.Campylobacter jejuni and Campylobacter coli are the most common causes of human bacterial gastroenteritis in industrialized countries (21). The flagellin-encoding genes flaA and flaB share 95% sequence homology and are arranged in tandem (9,20). While flaA gene expression appears critical for motility, colonization, and pathogenesis, this is not the case for flaB, which is thought to be a largely nonfunctional reservoir of genetic variation that can increase the diversity of flaA by recombination and so assist the cell in evading host immune responses (1,7,8,28).The flaA gene is commonly used for typing C. jejuni and C. coli. Two methods have gained wide acceptance: flaA restriction fragment length polymorphism (RFLP) (19) and flaA short variable region (SVR) sequencing (16). The flaA RFLP technique involves PCR amplification of the entire flaA gene followed by RFLP of the PCR product (19). Sequencing the SVR of the flaA gene was developed as a more streamlined and portable alternative to flaA RFLP protocols (16), and sequence variants are compiled at a central website (http://pubmlst.org/campylobacter /flaA/) (10). While both of these methods are very effective, they have several disadvantages: the RFLP approach is multistep, because the PCR product must be cleaved with a restriction enzyme, and the fragments must be subsequently resolved by electrophoresis (33). Also, there are many changes in the sequence that will not alter the sizes of the restriction fragments. SVR sequencing is also multistep; the targeted region is small, which limits resolving power; and DNA sequencing requires expensive equipment ...

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“…Antimicrobial discs (Oxoid, Basingstoke, UK) were placed on the inoculated MHB plates and they were incubated in the VAIN for 48 h. The following antimicrobial discs were used: aztreonam, tetracycline, gentamicin, cephalothin, amoxicillin-clavulanic acid, nalidixic acid, chloramphenicol, erythromycin (all 30 µg), streptomycin (25 µg), and ciprofloxacin (5 µg). Interpretation of the results was performed using the resistance breakpoint for Campylobacters described by others [14,15]. When no breakpoints were available for Campylobacters, the resistance breakpoint described by CLSI for Enterobacteriaceae was used.…”

Section: Antibiotic Susceptibility Testingmentioning

confidence: 99%

Grape Phenolic Extract Potentially Useful in the Control of Antibiotic Resistant Strains of <i>Campylobacter</i>

Mingo¹,

Carrascosa²,

Pascual‐Teresa³

et al. 2014

AiM

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In this work, a grape phenolic extract obtained by methanol extraction has been demonstrated to be effective in inhibiting the growth of different strains and species of Campylobacter, one of the most important bacterial foodborne pathogens causing gastroenteritis worldwide. Noteworthily, it was particularly effective against several strains presenting multiple antibiotic resistances. In all cases, the minimum inhibitory concentration (MIC) was lower than 300 mg GAE/L, being of 60 mg GAE/L for one of the most resistant strains (C. coli LP2), while the others were between 120 mg GAE/L and 180 mg GAE/L. The analytical study of the main phenolic compounds in the grape extract revealed that it was mainly constituted by catechins (85.7%) and phenolic acids (13.7%). However, experiments developed using pure standards demonstrate that phenolic acids (such as gallic, p-hidroxibenzoic, vanillic, and homovanillic acids) were the most active, provoking a Campylobacter growth decrease between 6.7 and 7.6 log, while epicatechin was the only catechin with activity as pure compound (1 log of growth decrease).

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Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from poultry in the South-East Queensland region

Abstract: This work has shown that the majority of C. jejuni and C. coli isolates were susceptible to the six antibiotics tested by both disc diffusion and MIC methods. Disc diffusion represents a suitable alternative methodology to agar-based MIC methods for poultry Campylobacter isolates.

Cited by 57 publications

References 15 publications

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Campylobacter jejuni and Campylobacter coli Genotyping by High-Resolution Melting Analysis of a flaA Fragment

Grape Phenolic Extract Potentially Useful in the Control of Antibiotic Resistant Strains of <i>Campylobacter</i>

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Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from poultry in the South-East Queensland region

Abstract: This work has shown that the majority of C. jejuni and C. coli isolates were susceptible to the six antibiotics tested by both disc diffusion and MIC methods. Disc diffusion represents a suitable alternative methodology to agar-based MIC methods for poultry Campylobacter isolates.

Cited by 57 publications

References 15 publications

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Prevalence, strain identification and antimicrobial resistance of Campylobacter spp. isolated from slaughtered pig carcasses in Brazil

Campylobacter jejuni and Campylobacter coli Genotyping by High-Resolution Melting Analysis of a flaA Fragment

Grape Phenolic Extract Potentially Useful in the Control of Antibiotic Resistant Strains of &lt;i&gt;Campylobacter&lt;/i&gt;

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Product

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About

Grape Phenolic Extract Potentially Useful in the Control of Antibiotic Resistant Strains of <i>Campylobacter</i>