Antibiotic Resistance in an Oral Isolate ofPrevotella intermedia

Genetic analysis of mobile tetQ elements in oral Prevotella species

“…The gene conferring tetracycline resistance in PDRC11 was cloned and determined by DNA sequence analysis to be tetQ, and was found to be chromosomally-encoded [18]. Using the sequence data available for PDRC11 tetQ , we designed a transposon capture strategy utilizing plasmid pFD665, a Bacteroides - E. coli shuttle vector originally used to clone and sequence a conjugative transposon (cTn341) from B. fragilis strain 341 [29].…”

“…The tetQ gene cloned from one Prevotella nigrescens clinical isolate was nearly identical in sequence to tetQ from a conjugative transposon identified in Bacteroides fragilis [18]. Tetracycline resistant clinical isolates of Prevotella and Porphyromonas have been shown to transfer tetracycline resistance to other bacterial species by conjugation in vitro [19–21].…”

PCR monitoring for tetracycline resistance genes in subgingival plaque following site‐specific periodontal therapy

“…Primers for tetQ were designed using the Prime program from the DNA analysis package Version 8 of the Genetics Computer Group (Genetics Computer Group, 575 Science Drive, Madison, WI). The tetQ primers were de-signed to match the tetQ gene from Prevotella intermedia (Walker & Bueno 1997) (Genbank accession . U73497) as well as the tetQ genes from Bacteroides fragilis (Genbank accession .…”

Oral colonization by anaerobic bacteria during childhood: role in health and disease

“…Another important aspect is that the normal microflora represents a reservoir of genes that are responsible for resistance to different antibiotics by several resistance mechanisms and that these genes can be transferred from antibiotic-resistant indigenous bacteria to human pathogens (Levy et al, 1988). In oral pigmented Prevotella spp., resistance genes seem to be located in conjugal elements and transferred via these elements between different bacterial species (Guiney and Bouic, 1990;Walker and Bueno, 1997). Besides these indirect consequences of the antibiotic resistance, the high frequencies of resistant anaerobic strains among the oral microflora may have a significant impact on the treatment outcome and practices of pediatric infections of oral origin.…”