2000
DOI: 10.1034/j.1600-051x.2000.027006437.x View full text |Buy / Rent full text
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Abstract: These results complement earlier studies of tet resistance and demonstrate the efficacy of PCR monitoring for the appearance of specific resistance genes during and after antibiotic therapy.

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“…The nucleotide sequence of the tetQ gene was almost identical among the clinical isolates, supporting the horizontal transfer of the gene [9]. Although PCR methods have already been used for the detection of tetQ gene in clinical plaque samples [7,10], quantitative monitoring of gene presence is required for analysis of its dynamics in periodontal pockets.…”
Section: Introductionmentioning
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“…The nucleotide sequence of the tetQ gene was almost identical among the clinical isolates, supporting the horizontal transfer of the gene [9]. Although PCR methods have already been used for the detection of tetQ gene in clinical plaque samples [7,10], quantitative monitoring of gene presence is required for analysis of its dynamics in periodontal pockets.…”
Section: Introductionmentioning
“…The prevalence of two such genes, tetM and tetQ , in the periodontal environment has been investigated, and the literature indicates that 81–84% of bacteria carrying tetM are not periodontal pathogens, while tetQ is carried mainly by Prevotella and Bacteroides spp. (Olsvik et al 1994, 1995, Lacroix & Walker 1996, Manch‐Citron et al 2000, Chung et al 2002). Another gene, tetW , also encoding ribosomal protection, has also been detected in the oral cavity as present in both pathogenic and non‐pathogenic species (Villedieu et al 2003).…”
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“…Manch‐Citron et al. (10), using polymerase chain reactions (PCR), found the tetracycline‐resistance genes tet (M), tet (O) and tet (Q) in samples derived from the subgingival plaque of patients undergoing tetracycline fibre therapy (10). Tetracycline resistance has been found to be relatively prevalent among enterococci isolated from the endodontic environment (2, 15).…”
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