Antibiotic resistance, genome analysis and further safe traits of Clostridium perfringens ICVB082; a strain capable of producing an inhibitory compound directed only against a closely related pathogenic strain

Vieco-Saiz, Nuria; Belguesmia, Yanath; Vachée, Anne; Maréchal, Caroline Le; Salvat, Gilles; Drider, Djamel

doi:10.1016/j.anaerobe.2020.102177

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…The 49 LAB strains isolated in this work were tested against C. perfringens DSM 756 as well as against six other Clostridium strains isolated from sick chickens, including C. perfringens ICVB081: a strain harboring the netB gene [32]. Two methods were used to assess the anti-C. perfringens activity: the slab test using the whole bacteria and their metabolites according to the protocol described by Dec et al [33]; and the wells test using the neutralized supernatant according to Bendali et al [34].…”

Section: Anti-clostridium Perfringens Activitymentioning

confidence: 99%

Protective Effects of Novel Lactobacillaceae Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: In Vitro and In Vivo Evidences

et al. 2022

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The present study aimed to show the benefits of novel lactic acid bacteria (LAB) strains isolated from the caeca of healthy chickens. These novel strains, identified as Limosilactobacillus reuteri and Ligilactobacillus salivarius, displayed high levels of lactic acid production, capability of biofilm formation, high aggregation and adhesion scores, and significant survival rates under conditions mimicking the chicken gastrointestinal tract (GIT). In addition, these novel Lactobacillaceae isolates were neither hemolytic nor cytotoxic. In vivo trials were able to establish their ability to reduce necrotic enteritis. Notably, a significant weight gain was registered, on day 10 of treatment, in the group of chickens fed with a mixture of L. reuteri ICVB416 and L. salivarius ICVB430 strains, as compared with the control group. This group has also shown a reduced number of lesions in the gut compared with other infected chicken groups. This study provides in vitro and in vivo evidence supporting the benefits of these novel Lactobacillaceae isolates for their use in poultry livestock as protective cultures to control the bacterial necrotic enteritis (NE) Clostridium perfringens.

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Section: Anti-clostridium Perfringens Activitymentioning

confidence: 99%

Protective Effects of Novel Lactobacillaceae Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: In Vitro and In Vivo Evidences

et al. 2022

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“…A mounting concern has been expressed over the antibiotics used in animal agriculture ( 10 ), therefore developing safe and practically sound preventive alternatives is an urgent need to control C. perfringens infections. Currently, an inactivated tetravalent enterotoxemia vaccine is available for the immunization of livestock by vaccine manufacturing branches of Razi Vaccine and Serum Research Institute, Iran.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Clostridium perfringens type D in calcium alginate beads: toxin production mimics free cell culture

Rakhshandeh¹,

Bafti²,

Familsatarian³

et al. 2022

IJM

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Background and Objectives: Cell-immobilization is used to maintain microbial culture to produce metabolites in repeat- ed-batch or continuous fermentations, thereby reducing the time and resources spent on delivering mass production of microbe. The technique also enables shortening of the detoxification phase and the amount of formaldehyde required due to low incidence of viable bacteria in the extract. Materials and Methods: A solution of sodium alginate containing Clostridium perfringens cells was dropped into stirring CaCl solution via a sterile syringe needle. Optimizations resulted in reasonably uniform beads containing C. perfringens. Beads were externally stabilized by poly L-lysine, followed by immersion in a solution of Na-alginate to coat them with a new layer of alginate forming an alginate-PLL-alginate cortex. Results: This study proved successful in immobilizing C. perfringens cells inside uniform alginate microspheres. Cell load- ing and cell propagation inside the beads were measured. The cell loaded beads were cultivable in liquid media producing 550 minimum lethal doses per milliliter (MLD/ml) in a 72 h. Conclusion: The research paved the way for further investigations to optimize and establish an efficient bacterial encapsu- lation method. Thus, it seems possible to produce toxins from beads engulfing C. perfringens on larger scales via repeat- ed-batch or continuous fermentation processes.

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Biodiversity and Phylogenetic Relationships of Novel Bacteriocinogenic Strains Isolated from Animal’s Droppings at the Zoological Garden of Lille, France

Eveno

Salouhi

Belguesmia

et al. 2020

Probiotics & Antimicro. Prot.

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Antibiotic resistance, genome analysis and further safe traits of Clostridium perfringens ICVB082; a strain capable of producing an inhibitory compound directed only against a closely related pathogenic strain

Cited by 7 publications

References 43 publications

Protective Effects of Novel Lactobacillaceae Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: In Vitro and In Vivo Evidences

Protective Effects of Novel Lactobacillaceae Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: In Vitro and In Vivo Evidences

Immobilization of Clostridium perfringens type D in calcium alginate beads: toxin production mimics free cell culture

Biodiversity and Phylogenetic Relationships of Novel Bacteriocinogenic Strains Isolated from Animal’s Droppings at the Zoological Garden of Lille, France

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