Protective Effects of Novel Lactobacillaceae Strains Isolated from Chicken Caeca against Necrotic Enteritis Infection: In Vitro and In Vivo Evidences

Abstract

The present study aimed to show the benefits of novel lactic acid bacteria (LAB) strains isolated from the caeca of healthy chickens. These novel strains, identified as Limosilactobacillus reuteri and Ligilactobacillus salivarius, displayed high levels of lactic acid production, capability of biofilm formation, high aggregation and adhesion scores, and significant survival rates under conditions mimicking the chicken gastrointestinal tract (GIT). In addition, these novel Lactobacillaceae isolates were neither hemolytic nor cytotoxic. In vivo trials were able to establish their ability to reduce necrotic enteritis. Notably, a significant weight gain was registered, on day 10 of treatment, in the group of chickens fed with a mixture of L. reuteri ICVB416 and L. salivarius ICVB430 strains, as compared with the control group. This group has also shown a reduced number of lesions in the gut compared with other infected chicken groups. This study provides in vitro and in vivo evidence supporting the benefits of these novel Lactobacillaceae isolates for their use in poultry livestock as protective cultures to control the bacterial necrotic enteritis (NE) Clostridium perfringens.

Assessment of biosecurity traits and in situ antibacterial activities of Weissella cibaria strains isolated from Campos das Vertentes, Minas Gerais, Brazil focused for a potential probiotic and food application

“…Assessing gastrointestinal tract survival by the agar enumeration does not take into account the viable but nonculturable cells (VBNC) [43]. Previous studies used the cytometry method, which includes both viable bacteria and VBNC and as expected, the survival percentages obtained by this method were signi cantly higher in comparison to the data obtained after their growth and enumeration by agar plating method [39].…”

“…We noted during the assessment of the survivability of Weissella strains in the gastrointestinal tract, a small loss of the viability of the cells after 1.5 h in conditions mimicking the stomach compartment, and also no survivability was observed upon 3 h of incubation in conditions simulating those of the duodenal compartment (Table 5). The ability of bacteria to survive under gastric digestion conditions is considered a key criterion for probiotic selection [39]. To overcome this drawback, strategies of delivery like encapsulation that could help the passage through the gastrointestinal tract by protecting bacteria from acidic stress and rendering their delivery process directly in the intestine where they can act as probiotics and provide health incentives to the host [40,41].…”

Recent Trends on Mitigative Effect of Probiotics on Oxidative-Stress-Induced Gut Dysfunction in Broilers under Necrotic Enteritis Challenge: A Review

“…Lactobacillus suppresses intestinal dysbiosis and maintains gut integrity owing to its various protective mechanisms: competitive exclusion, which prevents the adherence of pathogens to mucosal surfaces; lactic acid, disrupts the cell membrane which culminates in deleterious effects such as the inhibition of enzymatic activities; and the alteration of DNA structure and cell death [ 181 ]. Lactobacillus can form biofilms which can be used as barriers against enteropathogens, thus providing a conducive environment for gut cells’ proliferation and renewal [ 182 ]. Lactobacillus can produce bacteriocins against Salmonella enteric ATCC 25566, Yersinia enterocolitica ATCC 2371 and Bacillus cereus ATCC 49064, which could explain its antimicrobial activity [ 46 ].…”

New Recording of Lactic Acid Bacteria Strains from the Contents of Adult Chicken Intestines

“…While the bacterial strains Lactobacillus helveticus and Lactiplantibacillus plantarum agreed with the study (Jha et al, 2020). As for the bacterial strain Limosilactobacillus sp., it is Novel Lactobacillaceae strans (Vieco-Saiz et al, 2022). 9) the genetic distance of the local strain Lactiplantibacillus plantarum strain Al-Salhi-3 with the global strains.…”