Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile

Sacristán, Carlos; Esperón, Fernando; Acuña, Francisca; Aguilar, Emilio de la Cruz; García, Sebastián; López, María José; Cevidanes, Aitor; Neves, Elena; Cabello, Javier; Hidalgo-Hermoso, Ezequiel; Poulin, Élie; Millán, Javier; Napolitano, Constanza

doi:10.1016/j.scitotenv.2019.134900

Cited by 31 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This antimicrobial class is known to persist in the environment (76), and to resist biodegradation in wastewater-treatment processes and in media with elevated microbial activity, such as byproduct sludge (77, 78). Thus, the fact that such antimicrobial class presented more significant findings in the synanthropic coastal species (kelp gull), likely indicates higher ARGs pollution of coastal environments due to anthropogenic impact and environmental contamination (e.g., WWTP effluents and wastewater discharge) (10,16).…”

Section: Discussionmentioning

confidence: 99%

“…The same thermal cycle was used for all rtPCR reactions [6 ′ 95 • C, 40x (10 ′′ 95 • C, 30 ′′ 60 • C)], with alignment and extension in the same step, at constant 60 • C. A melting curve step was performed at the end of the rtPCR reaction (30). As per (37), we applied the term "multiresistant microbiome" when a fecal sample presented at least three ARGs encoding resistance to different classes of antimicrobials (10,29,30). All samples used in this study were collected as part of the Santos Basin Beach Monitoring Project (Projeto…”

Section: Sample Collectionmentioning

confidence: 99%

“…Microbial resistance is the result of natural bacteria genetic plasticity and interactions between microbial agents, host organisms and the environment (1,5), enhanced by the selective pressure exerted by antimicrobial usage and over-prescription in human and veterinary medicine treatments, animal and fish production (i.e., growth promoters and prophylaxis), agriculture and food technologies (1,5,6). The consequent remodeling of the existing microbiomes (group of all the genomic elements of a specific microbiota), associated with their dissemination capacity, confer antimicrobial resistance genes (ARGs) the role of environmental pollutants (7,8) and indicators of environmental anthropization (2,9,10).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, only a small fraction of bacteria are cultivable (21,22). Thus, in order to promote a more comprehensive approach, we employed highly sensitive real time polymerase chain reaction (rtPCR) methods (10,23) to directly detect and quantify 21 selected plasmid-mediated ARGs in the gastrointestinal microbiome of two wild seabirds species (kelp gulls [Larus dominicanus] and Magellanic penguins [Spheniscus magellanicus]) upon admission to a rehabilitation center. The goals of this study were to (i) assess the presence and load of ARGs in these individuals and (ii) evaluate our findings in light of selected biological and ecological parameters (i.e., dispersal [migratory and non-migratory], feeding niche [coastal and pelagic], and interaction with human-impacted areas [synanthropic and non-synanthropic]).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Ewbank

Esperón²,

Sacristán

et al. 2021

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance genes (ARGs) are environmental pollutants and anthropization indicators. We evaluated human interference in the marine ecosystem through the ocurrence and quantification (real-time PCRs) of 21 plasmid-mediated ARGs in enema samples of 25 wild seabirds, upon admission into rehabilitation: kelp gull (Larus dominicanus, n = 14) and Magellanic penguin (Spheniscus magellanicus, n = 11). Overall, higher resistance values were observed in kelp gulls (non-migratory coastal synanthropic) in comparison with Magellanic penguins (migratory pelagic non-synanthropic). There were significant differences between species (respectively, kelp gull and Magellanic penguin): ARGs occurrence (blaTEM [p = 0.032]; tetM [p = 0.015]; tetA [p = 0.003]; and sulII [p = 0.007]), mean number of ARGs per sample (p = 0.031), ARGs mean load percentage (aadA [p = 0.045], tetA [p = 0.031], tetM [p = 0.016], blaTEM [p = 0.032], sulII [p = 0.008]), percentage of genes conferring resistance to an antimicrobial class (betalactams [p = 0.036] and sulfonamides [p = 0.033]), mean number of genes conferring resistance to one or more antimicrobial classes (p = 0.024]), percentage of multiresistant microbiomes (p = 0.032), and clustering (p = 0.006). These differences are likely due to these species' contrasting biology and ecology - key factors in the epidemiology of ARGs in seabirds. Additionally, this is the first report of mecA in seabirds in the Americas. Further studies are necessary to clarify the occurrence and diversity of ARGs in seabirds, and their role as potential sources of infection and dispersal within the One Health chain of ARGs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Ewbank

Esperón²,

Sacristán

et al. 2021

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given the potential for wild animals to affect AMR persistence, spread, and evolution and the predicted subsequent risks for public health, there have been calls for the better integration of wildlife into current research on AMR (12), including the use of wild animals as indicators or biosentinels of AMR contamination in the environment (4,15). In particular, shared ARB, ARGs, and plasmids illustrate potential exchanges between humans, domestic animals, and environmental compartments (4,16,17).…”

mentioning

confidence: 99%

Merging Metagenomics and Spatial Epidemiology To Understand the Distribution of Antimicrobial Resistance Genes from Enterobacteriaceae in Wild Owls

Miller

Ponder

Willette

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a well-documented phenomenon in bacteria from many natural ecosystems, including wild animals. However, the specific determinants and spatial distribution of resistant bacteria and resistance genes (ARGs) in the environment remain incompletely understood. In particular, the relative importance of anthropogenic sources of AMR are lacking, compared to other biological and ecological influences. Here we conduct a cross-sectional study of AMR in great horned owls (Bubo virginianus) and barred owls (Strix varia) admitted to a rehabilitation center in the midwestern United States. A combination of selective culture enrichment and shotgun metagenomic sequencing was used to identify ARGs from Enterobacteriaceae. Overall, AMR prevalence was comparable to past studies of resistant Enterobacteriaceae in raptors, with acquired ARGs identified in 23% of samples. Multi-model regression analyses identified seasonality and owl age as important predictors of ARG likelihood, with birds sampled during warmer months more likely to harbor ARGs compared to those from cooler months, and hatch year birds more likely to harbor β-lactam ARGs than adults. Beyond host-specific determinants, ARG-positive owls were also more likely to be recovered from areas of high agricultural land cover. Spatial clustering analyses identified a significant high-risk cluster of tetracycline ARG-positive owls in the southern sampling range, but this could not be explained by any predictor variables. Taken together, these results highlight the complex distribution of AMR in natural environments and suggest that both biological and anthropogenic factors play important roles in determining the emergence and persistence of AMR in wildlife. IMPORTANCE Antimicrobial resistance (AMR) is a multifaceted problem that poses a worldwide threat to human and animal health. Recent reports suggest that wildlife may play an important role in the emergence, dissemination, and persistence of AMR. As such, there have been calls for better integration of wildlife into current research of AMR, including the use of wild animals as bio-sentinels of AMR contamination in the environment. A One Health approach can be used to gain a better understanding of all AMR sources and pathways, particularly those at the human-animal-environment interface. Our study focuses on this interface in order to assess the effect of human-impacted landscapes on AMR in a wild animal. This work highlights the value of wildlife rehabilitation centers for environmental AMR surveillance and demonstrates how metagenomic sequencing within a spatial epidemiology framework can be used to address questions surrounding AMR complexity in natural ecosystems.

show abstract

Assessing the benefits of composting poultry manure in reducing antimicrobial residues, pathogenic bacteria, and antimicrobial resistance genes: a field-scale study

Esperón¹,

Albero²,

Ugarte‐Ruiz

et al. 2020

Environ Sci Pollut Res

View full text Add to dashboard Cite

Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile

Cited by 31 publications

References 43 publications

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Merging Metagenomics and Spatial Epidemiology To Understand the Distribution of Antimicrobial Resistance Genes from Enterobacteriaceae in Wild Owls

Assessing the benefits of composting poultry manure in reducing antimicrobial residues, pathogenic bacteria, and antimicrobial resistance genes: a field-scale study

Contact Info

Product

Resources

About