Antibiotic resistance caused by permeability changes of the archaebacterium Methanococcus vannielii

Haas, E

doi:10.1016/0378-1097(86)90234-x

Cited by 10 publications

(13 citation statements)

References 9 publications

(11 reference statements)

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“…Exposure to mutating agents such as EMS and UV reduces the number of viable cells drastically as found in studies by Yang et al [92] and Haas et al [84] and a relatively large fraction of the surviving microbes was mutated. High lethality caused by such mutagenesis methods is not unique to methanogens.…”

Section: Genetic Systems In Methanogensmentioning

confidence: 85%

A comprehensive study into the molecular methodology and molecular biology of methanogenic Archaea

Lange

Ahring

2001

FEMS Microbiol Rev

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Methanogens belong to the kingdom of Euryarchaeota in the domain of Archaea. The Archaea differ from Bacteria in many aspects important to molecular work. Among these are cell wall composition, their sensitivity to antibiotics, their translation and transcription machinery, and their very strict demands to anaerobic culture conditions. These differences may, at least partly, be responsible for the delay in availability of genetic research tools for methanogens. At present, however, the research within genetics of methanogens and their gene regulation and expression is in rapid progress. Two complete methanogenic genomes have been sequenced and published and more are underway. Besides, sequences are known from a multitude of individual genes from methanogens. Standard methods for simple DNA and RNA work can normally be employed, but permeabilization of the cell wall may demand special procedures. Efficient genetic manipulation systems, including shuttle and integration vector systems, have appeared for mesophilic, but not for thermophilic species within the last few years and will have a major impact on future investigations of methanogenic molecular biology.

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Section: Genetic Systems In Methanogensmentioning

confidence: 85%

A comprehensive study into the molecular methodology and molecular biology of methanogenic Archaea

Lange

Ahring

2001

FEMS Microbiol Rev

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“…Some progress has been made in this direction for several species of archaebacteria. Several drug-resistant and auxotrophic mutants have been reported in various methanogen species (3,8,10,11,18, 20, 22). Among the halophilic archaebacteria, transfer mechanisms based on conjugation and transfection with DNA from a lytic phage have been demonstrated (6,17).…”

mentioning

confidence: 99%

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg

et al. 1988

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A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions. A significant amount of molecular information has been uncovered about methanogenic archaebacteria with the use of methanogen DNAs that have been cloned into eubacterial hosts (7, 9). A method for performing genetic experiments in methanogens is necessary so that the cloned genes can be used as starting points for meaningful physiological studies. Techniques for mutant selection and methods for introducing methanogen genes into their original backgrounds are the minimal requirements for a genetic system. Some progress has been made in this direction for several species of archaebacteria. Several drug-resistant and auxotrophic mutants have been reported in various methanogen species (3,8,10,11,18, 20, 22). Among the halophilic archaebacteria, transfer mechanisms based on conjugation and transfection with DNA from a lytic phage have been demonstrated (6,17). Genetic transformation of the mesophile Methanococcus voltae PS in liquid culture was reported recently (3).We present evidence for a natural transformation system in the thermophilic methanogen Methanobacterium thermoautotrophicum Marburg. A marker for 5-fluorouracil (FU) resistance was transformed into the wild-type strain via DNAs from drug-resistant mutants. MATERIALS AND METHODSStrains and media. Table 1 lists the strains used in this study. Halobacterium volcanii was grown in medium 97 at 37°C (5). Escherichia coli was grown in LB broth at 37°C. M. thermoautotrophicum strains were grown on mineral medium 2 as described previously (1, 24). Mineral medium plates contained 0.8% GELRITE gellan gum (Kelco Division of Merck & Co., Inc., San Diego, Calif.) plus a cation mixture composed of 1 g each of MgSO4 * 7H20 and CaC12 *2H20 per liter. The amount of Na2S -9H20 was reduced 10-fold to a final concentration of 0.006%. Mineral medium agar plates contained 2% Noble agar (Difco Laboratories, Detroit, Mich.) with or without the cation mixture. All plating operations, including preparation of plates after sterilization of anoxic medium, were done in an anaerobic chamber under an atmosphere of 95% N2 and 5% H2 (2). Additions were made by spreading liquid supplements on the solidified medium with a bent glass rod or a sterile plastic loop. Plates were incubated in pressure vessels (modified from references 1 and 2) under an atmosphere of H2-C02- H2S (80:19:1 [vol/vol]) at 200 to 300 kPa for 2 to 4 days at 60°C. To score colonies, the gas phase was exchanged with * Corresponding author. 653 nitrogen, and the vessels were brought into the anaerobic chamber.DNA purification. M. thermoautotrophicum cells were...

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“…Mutants that are resistant to 2-bromoethanesulfonic acid (BES), an analog of methyl-coenzyme M (CoM) and a cofactor in methanogenesis (3,31), have been reported for several methanogens including Methanosarcina barkeri (26), Methanobacterium thermoautotrophicum (17), Methanococcus vannielii (10), and Methanococcus voltae ( (30), and agar medium was prepared anaerobically as described by Jones et al (16). Complex medium (16) was used for all plating experiments and for maintenance of stock cultures.…”

mentioning

confidence: 99%

Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system

Santoro¹,

Konisky²

1987

J Bacteriol

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Mutants of Methanococcus voltae were isolated that were resistant to the coenzyme M (CoM; 2-mercaptoethanesulfonic acid) analog 2-bromoethanesulfonic acid (BES). The mutants displayed a reduced ability to accumulate [35S]BES relative to the sensitive parental strain. BES inhibited methane production from CH3-S-CoM in cell extracts prepared from wild-type sensitive or resistant strains. BES uptake requwred the presence of both CO2 and H2 and was inhibited by N-ethylmaleimide and several reagents that are known to disrupt energy metabolism. The mutants showed normal uptake of isoleucine and were not cross-resistant to either azaserine or 5-methyltryptophan and, thus, were neither defective in general energy-dependent substrate transport nor envelope permeability. Both HS-CoM and CH3-S-CoM prevented the uptake of BES and protected cells from inhibition by it. We propose that M. voltae has an energy-dependent, carrier-mediated uptake system for HS-CoM and CH3-S-CoM which can also mediate uptake of BES.

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Antibiotic resistance caused by permeability changes of the archaebacterium Methanococcus vannielii

Cited by 10 publications

References 9 publications

A comprehensive study into the molecular methodology and molecular biology of methanogenic Archaea

A comprehensive study into the molecular methodology and molecular biology of methanogenic Archaea

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg

Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system

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