Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from hospital wastewater

Abstract

BACKGROUND <br />Hospital wastewaters may contain antibiotic resistant bacteria such as Escherichia coli. These E. coli harbor integron genes that are responsible for antibiotic resistance. The purpose of the current study was to evaluate the frequency of class 1 and 2 integrons in environmental antibiotic resistant E. coli strains isolated from the hospital wastewaters in Tehran, Iran.<br /><br />METHODS<br />As a descriptive cross-sectional study, this research was performed from April to September 2015 on hospital wastewaters in Tehran. Bacterial isolation and identification was performed by standard biochemical and bacteriological procedures. Susceptibility testing was done by employing the disk diffusion method using different antibiotics. Total DNAs were extracted to evaluate the presence of class 1 and 2 integrons by using the polymerase chain reaction (PCR) method with specific primers. <br /><br />RESULTS<br />Fifty E. coli strains were isolated and identified from the wastewaters of 25 hospitals in Tehran. The phenotype results showed that 46 isolates (92%) were resistant to at least one antibiotic and 27 isolates (54%) were multidrug resistant. PCR showed that 35 (70%) and 20 (40%) of the isolates had class 1 and 2 integrons respectively and 14 isolates (28%) had both class 1 and class 2 integrons.<br /><br />CONCLUSION<br />This study has shown a considerable presence of class 1 and class 2 integrons in E. coli strains isolated from hospital wastewaters in Tehran. Proper antibiotics prescription and appropriate hospital wastewater treatment can prevent resistance genes in E. coli from circulating in the environment.

<p>Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt</p>

“…Our findings concurred with those reported elsewhere. [103][104][105][106][107] The high resistance of E. coli O157:H7, L. monocytogenes, and K. pneumoniae to tetracycline correlated to the extensive use of tetracycline in dairy farms for treatment of infectious diseases. 108 In the current study, all strains had a MAR index which ranged between 0.44-0.88, since it is well known that isolates which display a MAR index less than 0.2 are supposed to come from antibiotic-rare used sources.…”

Antimicrobial Resistance and Incidence of Integrons in Aeromonas Species Isolated from Diseased Freshwater Animals and Water Samples in Iran

“…Integron-inserted gene cassettes have been reported in Aeromonas species isolated from fish in different countries, including China [6,7], Thailand [8], USA [9], Australia [10], Mexico [11], and South Africa [12][13][14]. However, the prevalence of the class 1 integron in Iran's aquaculture industry has not been addressed, and most studies in this area have focused on the prevalence of class 1 and 2 integrons in clinical isolates and environmental samples [15][16][17][18][19][20].…”

Resíduo Hospitalar E Seu Impacto: Uma Revisão Da Literatura

“…Nas infeções ou resistência de microrganismos patógenos, bem como medicamentos que interferem na resistência aos antimicrobianos, destacam-se os antibióticos (DECRAENE et al, 2018;IWERIEBOR et al, 2015;LIEN et al, 2018;RANJBAR et al, 2018;LIEN et al, 2016;SAKKAS et al, 2019;FEKADU et al, 2015;AHN;CHOI, 2016;MATHYS et al, 2019;PAULSHUS et al, 2019;NG et al, 2018;FRÓES et al, 2016;PAULSHUS et al, 2019;NG et al, 2018;KAMATHEWATTA et al, 2019;MIRANDA et al, 2015;WEN et al, 2016;CAHILL et al, 2019;MAGALHÃES et al, 2016;GALLER et al,2018;RODRIGUEZ-MOZAZ et al, 2015;GOMI et al, 2017;LE et al, 2016;SHANKARAIAH et al, 2017;MEIR-GRUBER et al, 2016;KHAN;SÖDERQUIST;JASS, 2019;MOHD;MALIK, 2018;STÅLSBY LUNDBORG et al, 2015;ALAM;IMRAN, 2018;MARATHE et al, 2019;KALAISELVI et al, 2016;HRENOVIC et al, 2019;PERINI et al, 2018).…”