Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)

Kotlarska, Ewa; Łuczkiewicz, Aneta; Pisowacka, Marta; Burzyński, Artur

doi:10.1007/s11356-014-3474-7

Cited by 89 publications

(46 citation statements)

References 68 publications

(86 reference statements)

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“…This could be due to the fact that these antibiotics are most commonly produced and prescribed in human and veterinary medicine in Germany (GERMAP, and sulfamethoxazole/trimethoprim is not surprising in coliform bacteria isolated from surface water and the findings are consistent with published literature (Laroche et al, 2009;Ibekwe et al, 2011;Su et al, 2012;Koczura et al, 2012;Rosas et al, 2015;Kotlarska et al, 2015;Zhang et al, 2015). The assessment of antibiotic resistance in only water phase may lead to an underestimation of the health risk, as higher number of resistant E. coli have been reported in the sediment (Rosas et al, 2015).…”

Section: Discussionsupporting

confidence: 74%

“…However, the frequency of occurrence was similar to that of class 2 integrons in E. coli isolated (1.43 %) from the Seine estuary in France (Laroche et al, 2009). The prevalence of class 2 integrons is generally low compared to class 1 in the reported literature (Koczura et al, 2012;Kotlarska et al, 2015;Su et al, 2012).…”

Section: Usa 4 % Of the Tetracycline Resistant Isolates Were Positivementioning

confidence: 89%

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Antibiotic resistance and virulence genes in coliform water isolates

Stange

Sidhu

Tiehm

et al. 2016

International Journal of Hygiene and Environmental Health

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Please cite this article as: Stange, C., Sidhu, J.P.S., Tiehm, A., Toze, S., Antibiotic resistance and virulence genes in coliform water isolates.International Journal of Hygiene and Environmental Health http://dx.doi.org/10. 1016/j.ijheh.2016.07.015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1Antibiotic resistance and virulence genes in coliform water isolates Graphical abstract2 ABSTRACTWidespread fecal pollution of surface water may present a major health risk and a significant pathway for dissemination of antibiotic resistance bacteria. The River Rhine is one of the longest and most important rivers in Europe and an important raw water source for drinking water production. A total of 100 coliform isolates obtained from River Rhine (Germany) were examined for their susceptibility to seven antimicrobial agents. Resistances against amoxicillin, trimethoprim/sulfamethoxazole and tetracycline were detected in 48 %, 11 % and 9 % of isolates respectively. The antibiotic resistance could be traced back to the resistance genes blaTEM, blaSHV, ampC, sul1, sul2, dfrA1, tet(A) and tet (B). Whereby, the ampC gene represents a special case, because its presence is not inevitably linked to a phenotypic antibiotic resistance. Multiple antibiotics resistance was often accompanied by the occurrence of class 1 or 2 integrons. E. coli isolates belonging to phylogenetic groups A and B1(commensal) were more predominant (57 %) compared to B2 and D groups (43 %) which are known to carry virulent genes. Additionally, six E. coli virulence genes were also detected. However, the prevalence of virulence genes in the E. coli isolates was low (not exceeding 4.3 % per gene) and no diarrheagenic E. coli pathotypes were detected. This study demonstrates that surface water is an important reservoir of ARGs for a number of antibiotic classes such as sulfonamide, trimethoprim, betalactam-antibiotics and tetracycline. The occurrence of antibiotic resistance in coliform bacteria isolated from River Rhine provides evidence for the need to develop management strategies to limit the spread of antibiotic resistant bacteria in aquatic environment.

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Section: Discussionsupporting

confidence: 74%

Section: Usa 4 % Of the Tetracycline Resistant Isolates Were Positivementioning

confidence: 89%

Antibiotic resistance and virulence genes in coliform water isolates

Stange

Sidhu

Tiehm

et al. 2016

International Journal of Hygiene and Environmental Health

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“…Wastewater treatment plants are considered as an important reservoir (hot-spots) of antibiotic-resistant bacteria (ARB), and antibiotic resistance genes (ARGs) [12][13][14] representing a major source of their dissemination in the environment [15,16]. The wastewater flowing into WWTPs create favorable conditions for the survival of ARB and transfer of ARGs, due to the high content of both microbial biomass and the nutrients [13].…”

Section: Introductionmentioning

confidence: 99%

Impact of type of wastewater treatment process on the antibiotic resistance of bacterial populations

et al. 2017

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Abstract. Antibiotic resistant bacteria reach the environment directly with faeces, and indirectly with sewage discharged from wastewater treatment plants (WWTPs). The aim of the study was to determine the level of removal of bacteria during wastewater treatment. Samples of untreated and treated sewage were collected from 13 WWTPs with different capacity, modification of treatment and type of inflowing wastewater. Microbiological characterization of the samples included determination of the total number of bacteria resistant (ARB) to β-lactams and tetracyclines as well as the number of Escherichia coli resistant to the same drugs. The counts of ARB and E. coli were determined on TSA and mFc media with/without antibiotic supplementation, respectively. The highest percent of reduction in number of ARB (at least 99.9%) and E. coli (above 99.3%) was obtained for WWTPs with A 2 O system. The lowest percentage reduction of ARB and E. coli was observed for WWTPs operating with SBR system. The lowest number of microorganisms resistant to analyzed antibiotics was observed for ARB and E. coli resistant to cefotaxime and doxycycline. The results indicate a large variation in the removal of antibiotic-resistant bacteria in WWTPs depending on modifications of treatment system and type of inflowing wastewater.

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“…A variety of methods have been used to indicate human sewage contamination, such as the use of culturable antibiotic-resistant strains of bacteria, which tend to be more prevalent in wastewater (16)(17)(18)(19)(20). Additionally, many field studies have utilized microbial source tracking (MST) methods such as host-associated molecular markers to identify multiple sources of contamination (e.g., human, dog, gull, cow) (21)(22)(23)(24)(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

Comparison of Microbial and Chemical Source Tracking Markers To Identify Fecal Contamination Sources in the Humber River (Toronto, Ontario, Canada) and Associated Storm Water Outfalls

Staley

Grabuski

Sverko

et al. 2016

Appl Environ Microbiol

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Storm water runoff is a major source of pollution, and understanding the components of storm water discharge is essential to remediation efforts and proper assessment of risks to human and ecosystem health. In this study, culturable Escherichia coli and ampicillin-resistant E. coli levels were quantified and microbial source tracking (MST) markers (including markers for general Bacteroidales spp., human, ruminant/cow, gull, and dog) were detected in storm water outfalls and sites along the Humber River in Toronto, Ontario, Canada, and enumerated via endpoint PCR and quantitative PCR (qPCR). Additionally, chemical source tracking (CST) markers specific for human wastewater (caffeine, carbamazepine, codeine, cotinine, acetaminophen, and acesulfame) were quantified. Human and gull fecal sources were detected at all sites, although concentrations of the human fecal marker were higher, particularly in outfalls (mean outfall concentrations of 4.22 log 10 copies, expressed as copy numbers [CN]/ 100 milliliters for human and 0.46 log 10 CN/100 milliliters for gull). Higher concentrations of caffeine, acetaminophen, acesulfame, E. coli, and the human fecal marker were indicative of greater raw sewage contamination at several sites (maximum concentrations of 34,800 ng/liter, 5,120 ng/liter, 9,720 ng/liter, 5.26 log 10 CFU/100 ml, and 7.65 log 10 CN/100 ml, respectively). These results indicate pervasive sewage contamination at storm water outfalls and throughout the Humber River, with multiple lines of evidence identifying Black Creek and two storm water outfalls with prominent sewage cross-connection problems requiring remediation. Limited data are available on specific sources of pollution in storm water, though our results indicate the value of using both MST and CST methodologies to more reliably assess sewage contamination in impacted watersheds. IMPORTANCEStorm water runoff is one of the most prominent non-point sources of biological and chemical contaminants which can potentially degrade water quality and pose risks to human and ecosystem health. Therefore, identifying fecal contamination in storm water runoff and outfalls is essential for remediation efforts to reduce risks to public health. This study employed multiple methods of identifying levels and sources of fecal contamination in both river and storm water outfall sites, evaluating the efficacy of using culture-based enumeration of E. coli, molecular methods of determining the source(s) of contamination, and CST markers as indicators of fecal contamination. The results identified pervasive human sewage contamination in storm water outfalls and throughout an urban watershed and highlight the utility of using both MST and CST to identify raw sewage contamination. S torm water runoff has been identified as one of the most prominent non-point sources of both biological and chemical contaminants, degrading water quality and posing risks to public health in impacted recreational waters (1-3). The high level of fecal contamination present in storm water ru...

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Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)

Cited by 89 publications

References 68 publications

Antibiotic resistance and virulence genes in coliform water isolates

Antibiotic resistance and virulence genes in coliform water isolates

Impact of type of wastewater treatment process on the antibiotic resistance of bacterial populations

Comparison of Microbial and Chemical Source Tracking Markers To Identify Fecal Contamination Sources in the Humber River (Toronto, Ontario, Canada) and Associated Storm Water Outfalls

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