Antibiotic resistance and molecular subtypes of clinical methicillin-resistant Staphylococcus aureus in a teaching hospital

Zeinali, Elham; Moniri, Rezvan; Musavi, GH

doi:10.4103/0255-0857.83926

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…SCCmec type IV was less common than other genotypes since 19.2% of the strains were SCCmec type IV. Such a diversity was also found in Iran (15), where 3.4% of MRSA strains were found to be SCCmec type I, 13.8% SCCmec type II, 9.2% SCCmec type IVb, 4.6% SCCmec type IVd and 3.4% SCCmec type V. In the same study, there was also a high frequency of non-typeable strains as 58.6% of MRSA strains were non-typeable. However, such diversity was not found in in Turkey since the study showed that 22 MRSA isolates which were tested for SCCmec types were SCCmec type III (16).…”

Section: Discussionsupporting

confidence: 67%

Prevalent Genotypes of Staphylococcus aureus Strains Isolated From Healthcare Workers in Duhok City, Kurdistan Region, Iraq

Hussein

2016

Int J Infect

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Background: Nasal carriage of Staphylococcus aureus and its virulence determinants are major risk factors for subsequent infections and transmission. Objectives: The current study aimed to determine the prevalence of panton-valentine leukocidin (PVL) gene, the SCCmec types, and the prevalent methicillin-resistant Staphylococcus aureus (MRSA) genotypes in strains isolated from healthcare workers (HCWs). Materials and Methods: DNA was extracted from 114 frozen S. aureus strains amongst them MRSA isolates were identified by conversional methods and PVL; genotyping were determined using PCR and pulsed-field gel electrophoresis (PFGE). Results: Fifty-nine out of 114 (51.7%) isolates were MRSA carrying mecA, 18/59 (31%) harboured SCCmec type I, 12/59 (20.1%) harboured SCCmec type II and 12/59 (20.1%) harboured type III. The pvl gene was detected in 10 S. aureus isolates, six MRSA and four MSSA. PFGE clustered the 114 isolates of S. aureus into eight predominant types (designated A -H). The most prevalent PFGE type was type A (n = 44, 37.7%). Conclusions: More than a quarter of the strains harboured SCCmec type I. Staphylococcus aureus isolates were clustered into eight predominant PFGE pulsotypes. The current study should be considered preliminary and further use of molecular studies to monitor the epidemiology of MRSA and its evolution is highly recommended.

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Section: Discussionsupporting

confidence: 67%

Prevalent Genotypes of Staphylococcus aureus Strains Isolated From Healthcare Workers in Duhok City, Kurdistan Region, Iraq

Hussein

2016

Int J Infect

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“…Also, in a hospital survey in the neighboring country, Pakistan, in 2010, the SCC mec types I and II were the predominant isolates with 60% and 40% rates, respectively ( 25 ). Some other studies in Iran have also reported the relative prevalence of the SCC mec types I and II ( 26 , 27 ). Fatholahzadeh et al ( 3 ) and Japoni et al ( 8 ) in two related studies in Iran reported the SCC mec type III as the most predominant type: rates of 98% in the former and 74.3% in the latter.…”

Section: Discussionmentioning

confidence: 96%

Emergence of SCCmec Type I Obtained from Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran

Ebrahim‐Saraie

Motamedifar

Sarvari

et al. 2015

Jundishapur J Microbiol

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Background:Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a major cause of nosocomial infections. Methicillin resistance in S. aureus is caused by the acquisition of the mecA gene, located on a mobile genetic element called the staphylococcal cassette chromosome (SCC).Objectives:The aim of this study was to evaluate the presence of the predominant SCCmec type present among clinical isolates.Materials and Methods:This cross-sectional study was performed on a total of 146 MRSA isolates obtained from clinical specimens between 2012 and 2013 from two major hospitals in Shiraz, Southwest of Iran. Antibiotic susceptibility profiles were determined by the disc diffusion method according to the guidelines of The Clinical and Laboratory Standards Institute. Bacterial DNA was extracted using the small-scale phenol-chloroform extraction method and was employed as polymerase chain reaction (PCR) templates for the assigned current SCCmec types.Results:The assigned SCCmec types by PCR revealed the SCCmec type I as the predominant type with 86 (58.9%) samples, followed by the SCCmec type II with 29 (19.9%), type III with 16 (11.0%), and type IV with 12 (8.2%) samples, respectively. The SCCmec type I MRSA isolates were significantly recovered from blood (80%) and sputum (67.2%). The results of antibacterial susceptibility tests for the MRSA isolates showed that all of those carrying the SCCmec type I and II had significantly greater resistance rates to Gentamicin and Rifampin than the isolates containing the SCCmec type III. Also, a significant difference was detected for susceptibility to Co-trimoxazole between the SCCmec type I and II MRSA isolates and the SCCmec type III, which was more resistant.Conclusions:The frequency of the isolates containing type I in the current study can indicate an emergence of this SCCmec type in the studied medical centers.

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Methicillin Resistant Staphylococcus aureus (MRSA) Strains and the Staphy‌lococcal Cassette Chromosome mec Types in Iran

Ghasemian¹,

Mirzaee²

2016

iem

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Antibiotic resistance and molecular subtypes of clinical methicillin-resistant Staphylococcus aureus in a teaching hospital

Cited by 4 publications

References 5 publications

Prevalent Genotypes of Staphylococcus aureus Strains Isolated From Healthcare Workers in Duhok City, Kurdistan Region, Iraq

Prevalent Genotypes of Staphylococcus aureus Strains Isolated From Healthcare Workers in Duhok City, Kurdistan Region, Iraq

Emergence of SCCmec Type I Obtained from Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran

Methicillin Resistant Staphylococcus aureus (MRSA) Strains and the Staphy‌lococcal Cassette Chromosome mec Types in Iran

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