Antibiotic resistance and determination of resistant genes among cockle (Anadara granosa) isolates of Vibrio alginolyticus

Shahimi, Safiyyah; Elias, Aishah; Mutalib, Sahilah Abd.; Salami, Mokry; Fauzi, Fazlina; Zaini, Nurul Aqilah Mohd; Ghani, Ma`Aruf Abd.; Azuhairi, A A

doi:10.1007/s11356-021-13665-4

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…Drug resistance carried by bacteria will flow from the food chain into human body via aquatic products (Chen et al, 2020). Human infection with drug-resistant bacteria will limit the choice of antibiotics, resulting in difficult treatment, which poses a threat to human health (Shahimi et al, 2021). Therefore, it is necessary to find alternative methods to control V. alginolyticus strains harmful to aquatic animals.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to V. alginolyticus representing a conditional pathogen of fish, it poses a serious threat to human public health and threatens the Chinese aquaculture industry, with reports of it causing human food poisoning, otitis media, septicemia, and other diseases (Li et al, 2009;Bunpa et al, 2018). Due to the large-scale outbreak of V. alginolyticus and the long-term overuse and abuse of a large number of antibiotics and other chemical drugs, drug resistance of Vibrio is increasing daily with major implications to the aquaculture industry (Shahimi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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A T3SS Regulator Mutant HY9901 ΔaraC of Vibrio alginolyticus Decreased the Expression of HopPmaJ and Provided Protection to Danio rerio as a Live-Attenuated Vaccine

et al. 2022

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Vibrio alginolyticus, a zoonotic bacterial pathogen, expresses a type III secretion system (T3SS) that is critical for pathogen virulence and disease development. In this study, the mutant HY9901 ΔaraC was obtained from the laboratory and its biological characteristics were analyzed. The swimming ability of ΔaraC decreased and exhibited a 2,600 times reduction in virulence to zebrafish. However, ΔaraC showed no difference in growth and extracellular protease activity compared to wild type. Biofilm-forming ability was improved at 24 h, but no difference was observed at other time points. The results of drug sensitivity testing showed that compared with the wild-type HY9901 strain, ΔaraC was sensitive to amikacin, tetracycline, neomycin, minocycline, and gentamicin. The transcription levels of T3SS effector proteins HopPmaJ, VopS, VcrV, and VopN were analyzed by qRT-PCR. The results showed that ΔaraC had significantly upregulated the mRNA expression of VopS, VcrV, and VopN, but significantly downregulated the mRNA expression of HopPmaJ at each stage compared with HY9901. Western blotting and the β-galactosidase reporter gene experiment also showed that the deletion of araC gene significantly downregulated the expression of HopPmaJ. Finally, the relative percent survival (RPS) rate of grouper inoculated by intramuscular (IM) injection of HY9901 ΔaraC was 61.3% after being challenged with HY9901. Real-time qPCR analysis showed that vaccination of HY9901 ΔaraC could enhance the expression of immune-related genes, including gata-1, il6, IgM, il-1β, and lyz in liver and spleen, indicating that ΔaraC applied as a live-attenuated vaccine effectively induced an immune response in the zebrafish. This study provides evidence for the subsequent development of an effective live-attenuated V. alginolyticus vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A T3SS Regulator Mutant HY9901 ΔaraC of Vibrio alginolyticus Decreased the Expression of HopPmaJ and Provided Protection to Danio rerio as a Live-Attenuated Vaccine

et al. 2022

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“…Although V. alginolyticus is sensitive to most antibiotics, it is highly resistant to antibiotics such as penicillin, erythromycin, vancomycin, ampicillin, sulfisoxazole, and sulfonamides [15,18,19]. Shahimi [20] et al identified the presence of four antibiotic resistance genes for penicillin (pbp2a), ampicillin (blaOXA), erythromycin (ermB) and vancomycin (vanB) by polymerase chain reaction. This is also one of the reasons for the repeated infection of V. alginolyticus in mariculture, which is difficult to treat, with wide damage area, long disease course and high mortality.…”

Section: Introductionmentioning

confidence: 99%

Research Progress on Virulence Factors of Vibrio alginolyticus: A Key Pathogenic Bacteria of Sepsis

Cai¹,

Yu²,

Li³

et al. 2024

Heat Illness and Critical Care

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As an opportunistic pathogen, V. alginolyticus is commonly found in people with weak immune systems or open wounds. The history of seafood exposure is a major feature of V. alginolyticus infection. V. alginolyticus can infect marine economic animals such as fish, shrimp, and shellfish, and is also one of the key pathogens that cause sepsis in human. Because of its rapid progress and extremely high mortality after the infection, it has received more and more attention in clinical practice. At present, there is no effective method to completely control the incidence of V. alginolyticus. Therefore, it is particularly important to study the virulence factors and pathogenic mechanisms of V. alginolyticus. This article reviews recent studies on virulence factors of V. alginolyticus, such as quorum sensing, virulence proteins, ferroportin hemolysin, flagella, lipopolysaccharide system and biofilm formation, with the hope of providing further insights into aquaculture and public health.

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Antibiotic resistance, virulence and genetic characteristics of Vibrio alginolyticus isolates from aquatic environment in costal mariculture areas in China

Wang

et al. 2022

Marine Pollution Bulletin

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Antibiotic resistance and determination of resistant genes among cockle (Anadara granosa) isolates of Vibrio alginolyticus

Cited by 5 publications

References 36 publications

A T3SS Regulator Mutant HY9901 ΔaraC of Vibrio alginolyticus Decreased the Expression of HopPmaJ and Provided Protection to Danio rerio as a Live-Attenuated Vaccine

A T3SS Regulator Mutant HY9901 ΔaraC of Vibrio alginolyticus Decreased the Expression of HopPmaJ and Provided Protection to Danio rerio as a Live-Attenuated Vaccine

Research Progress on Virulence Factors of Vibrio alginolyticus: A Key Pathogenic Bacteria of Sepsis

Antibiotic resistance, virulence and genetic characteristics of Vibrio alginolyticus isolates from aquatic environment in costal mariculture areas in China

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