Antibiotic Residues in Iranian Honey by Elisa

Mahmoudi, Razzagh; Norian, Reza; Pajohi‐Alamoti, Mohammadreza

doi:10.1080/10942912.2013.809539

Cited by 31 publications

(19 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, 2 of the local honey samples had tylosin B residues. In a study in which Mahmoudi et al (18) determined antibiotic residues in honey in Iran using the ELISA method, it was reported that the samples obtained in the summer and autumn were detected with tylosin residues whereas the samples obtained in the spring had no tylosin residues. The majority of the researchers have examined the existence of antibiotics residues by biochip array technology (16,20,23,24,34).…”

Section: Resultsmentioning

confidence: 99%

“…Screening methods are the first step in controlling antibiotic residues in food (12). There are various techniques for screening residues in foods of animal origin, the main ones being immunological methods: ELISA test kits, radioimmunoassay, multiarray biosensors (4,11,14,16,18,20,22,34) and chromatographic methods: high performance thinlayer chromatography (HPTLC), or high performance liquid chromatography (HPLC) (26,28,35). Biochip array biosensors have been commonly used due to reasons such as ease of use, rapid results, simultaneous multi-residue analysis, full automation and high efficiency (33).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Aksoy

2019

Medycyna Weterynaryjna

View full text Add to dashboard Cite

The existence of antibiotic residues in food causes serious health problems in humans. This study aims to determine antibiotic residues in honey intended for consumption. Evidence Investigator™ Biochip Array technology enables simultaneous quantitative detection of multiple analytes on a single sample. The Anti Microbial Array IV test kit is used in simultaneously detecting the quantity of 12 different groups of antimicrobials in honey samples. A competitive chemiluminescent immunoassay was employed for the detection of antimicrobials. The light signal produced from each test region on the biochip was detected using digital imaging technology. In this study, a total of 45 honey samples including 5 brands of honey and 40 local honey were analyzed. The honey samples were simultaneously screened for 12 different types of antibiotic residues by using the Evidence Investigator™ Anti Microbial Array IV test kit. In the honey samples examined, residues of erythromycin, streptomycin, amikacin, lincosamides, tylosin B and neomycin were detected. 91% of samples involved erythromycin residues whereas 15% of the local samples included streptomycin residues above the recommended concentration.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Aksoy

2019

Medycyna Weterynaryjna

View full text Add to dashboard Cite

show abstract

“…Such high residues must be due to the administration of large doses of CAP to honey bees. In a survey of antibiotic residues in Iranian honeys [11] (Mahmoudi et al 2014), 7.40% of autumn honey samples were contaminated with CAP, most likely because of the application of CAP in an effort to resist the high incidence of disease in autumn. In that investigation, it confirmed that water pollution is another factor attributable to the detection of CAP residues in honey samples.…”

Section: Discussionmentioning

confidence: 99%

Survey of Chloramphenicol in Honeys from China

Zhao¹,

Cheng²,

Gao³

et al. 2016

JRST

View full text Add to dashboard Cite

chloramphenicol (caP) is a broad-spectrum antibiotic and a potential carcinogen that has been banned for use in apiculture in china and other countries. The aim of this study was to investigate the occurrence of caP residues in various chinese honeys. in total, 219 raw honeys and 149 commercial honeys from china produced during four consecutive years (2011)(2012)(2013)(2014) were analyzed by eLiSa for the detection of caP residues. The results showed the percentage of positive samples of caP of 12.33% in the 219 raw honeys, withan obvious decreasefrom 2012 (20.97%) to 2014 (7.27%), the incidence of CAP showed a significant decrease. The CAP residues in all raw honeys ranged from nD (not detected) to 1.34 μg/kg, with a mean value of 0.08 μg/kg. The mean incidence of caP in 149 commercial honeys was 6.04%. From 2012 (8.82%) to 2014 (2.78%), the incidence of caP in commercial honeys showed an obvious decrease.

show abstract

“…In the literature there are studies on the development of ELISA methods for the determination of antibiotics (Burkin et al, 2018), as well as studies on their application on real samples (Shen and Jiang, 2005;Mahmoudi et al, 2014), but there are no data on similar evaluations. Certainly, the European requirements for screening methods are stricter (EC, 2002), and these methods should be examined in order to determine detection capability, as well as check them by using reference materials based on honey matrices and participation in proficiency tests.…”

Section: Rsd-relative Standard Deviation N-number Of Measurementsmentioning

confidence: 99%

Evaluation of ELISA tests as screening methods for determination of antibiotics and sulfonamides in honey

Jaksic¹,

Mihaljev²,

Kartalović³

et al. 2018

Food & Feed Res

View full text Add to dashboard Cite

The health safety of honey includes the correctness in terms of the presence of different contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in food. In this paper the application of ELISA tests for determination of selected antibiotics and sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was evaluated after application on honey samples spiked with standard solution of a particular analyte. Samples were prepared according to the instructions of the ELISA test manufacturer referring to honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory accuracy (73-111%) and precision (14-16%). Recovery for sulfametoxypyridazin was low (40%), and for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in relation to the requirements of the legislation (0.075-3 µg/kg). The test kits can be used to screen the presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the obtained validation parameters.

show abstract

Antibiotic Residues in Iranian Honey by Elisa

Cited by 31 publications

References 12 publications

Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Simultaneous screening of antibiotic residues in honey by biochip multi-array technology

Survey of Chloramphenicol in Honeys from China

Evaluation of ELISA tests as screening methods for determination of antibiotics and sulfonamides in honey

Contact Info

Product

Resources

About