Antibiotic monitoring in body fluids

Rouan, Marie-Claude

doi:10.1016/0378-4347(85)80202-4

Cited by 47 publications

(8 citation statements)

References 157 publications

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“…As a simple clean-up technique to replace the conventional liquidliquid partition [7,9,25,26], commercial reversed-phase (pre-packed C18 ) cartridges have been used [22,23,27,28]. On the other hand, Ikai et al [29] reported a rapid clean-up technique for residual SDD by using a normal-phase type of cartidge.…”

Section: Clean-upmentioning

confidence: 99%

“…It has been previously reported that SDD could be determined by HPLC using a reversed-phase column and a mixture of acetonitrile, acetic acid and water [211; however, OTC is not easy to detect as a tailing peak [22,23]. To separate both compounds without the tailing peak of OTC, the optimum HPLC conditions were examined by using an end-capped C 8 column, LiChrospher 100 RP-8, and by applying the above mixture as the mobile phase.…”

Section: Hplc Operating Conditionsmentioning

confidence: 99%

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High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs

Furusawa

1999

Chromatographia

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Key WordsColumn liquid chromatography Sep-Pak CN cartridge Oxytetracycline Sulphadimidine Meat and eggs SummaryA rapid method for the simultaneous determination/identification of residual oxytetracycline (OTC) and sulphadimidine (SDD) in meats (beef, pork, chicken) and eggs by high-performance liquid chromatography (HPLC) was developed. The extraction of OTC and SDD was performed using a Sep-Pak | CN cartridge. The extracts contained OTC/SDD analytes when examined by HPLC using a LiChrospher | 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4:68, v/v/v) with a photodiode array detector. The average recoveries from spiked samples (0.1 ~g g-1 and 1.0 ~g g-l) were in excess of 80.2 % with coefficients of variation between 1.5 and 5.0 %. The limits of detection for OTC and SDD were 0.05 and 0.02 ~g g-l, respectively.

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Section: Clean-upmentioning

confidence: 99%

Section: Hplc Operating Conditionsmentioning

confidence: 99%

High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs

Furusawa

1999

Chromatographia

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“…It is possible for this drug to remain in a high concentration in the blood of aged persons and those with decreased renal function. For establishing its dosage so that side effects can be prevented and the trough can be maintained, too, the determination of its concentration in blood is essential [3,4]. CFPM is excreted into urine by 80-89 % within 24 hours after its administration and no active metabolite which has an anti-microbial effect has been observed.…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation for the Direct Determination of Cefepime in Human Serum by Micellar Electrokinetic Capillary Chromatography

Kitahashi¹,

Furuta

2006

CPA

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A method for determining the concentration of cefepime, a cephem anti-microbial agent, by direct serum injection using micellar electrokinetic capillary chromatography is developed and its validation of the assay is studied. A borate buffer (100 mM; pH 8.0) containing sodium dodecyl sulfate (80 mM) is used as a run buffer. Ultraviolet detection is carried out at 260 nm. The migration time of cefepime is about 5.2 min. The limit of detection is 0.5 mg/l (S/N=3). The RSD's of intra-day and inter-day precisions are 1.57-4.08 % (6.8-94.2 mg/l) and 1.47-2.75 % (10.2-81.5 mg/l), respectively, and the recovery rate is 94-108%. 13 other cephem anti-microbial agents are measured under the analytical conditions of this method and the results show that cefatrizine, whose migration time coincides with that of cefepime, interferes with the determination of cefepime. This method is characterized by the ability of allowing analysis by the direct injection of serum samples of micro-quantities into the capillary.

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“…In response, the EU have assigned maximum residue limits (MRL) 1 for ␤-lactams in foods of 50 to 300 g/kg, depending on the ␤-lactam involved. Established methods of monitoring ␤-lactam levels in foods have included the relatively time-consuming bacterial growth inhibition assays (3) or expensive and labor-intensive HPLC techniques (4). An antibody-based method of detection, especially one with a broad specificity, could offer the advantages of simplicity, speed, low cost, and, additionally, the requirement for special equipment would be reduced.…”

mentioning

confidence: 99%

Investigation of the Interactions between β-Lactams and a Metallo-β-lactamase from Bacillus cereus Using a Monoclonal Antibody

Chambers

Wyatt

Morgan

2001

Analytical Biochemistry

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Antibiotic monitoring in body fluids

Cited by 47 publications

References 157 publications

High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs

High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs

Method Development and Validation for the Direct Determination of Cefepime in Human Serum by Micellar Electrokinetic Capillary Chromatography

Investigation of the Interactions between β-Lactams and a Metallo-β-lactamase from Bacillus cereus Using a Monoclonal Antibody

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