High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs

Abstract

Key WordsColumn liquid chromatography Sep-Pak CN cartridge Oxytetracycline Sulphadimidine Meat and eggs SummaryA rapid method for the simultaneous determination/identification of residual oxytetracycline (OTC) and sulphadimidine (SDD) in meats (beef, pork, chicken) and eggs by high-performance liquid chromatography (HPLC) was developed. The extraction of OTC and SDD was performed using a Sep-Pak | CN cartridge. The extracts contained OTC/SDD analytes when examined by HPLC using a LiChrospher | 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4:68, v/v/v) with a photodiode array detector. The average recoveries from spiked samples (0.1 ~g g-1 and 1.0 ~g g-l) were in excess of 80.2 % with coefficients of variation between 1.5 and 5.0 %. The limits of detection for OTC and SDD were 0.05 and 0.02 ~g g-l, respectively.

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

“…These limits require sensitive and specific methods for the detection of OTC in food-producing animals. Various methods have been reported, most of which are based on chemical methods such as high-performance liquid chromatography (HPLC) (Furusawa, 1999;Cinquina et al, 2003;Yuan et al, 2010), liquid chromatographymass spectroscopy (LC-MS) (Carson et al, 1998;Cherlet et al, 2003), and capillary electrophoresis (Huang et al, 1997). Although these methods offer a high precision and accuracy, they require expensive equipment and well-trained professionals.…”

“…Moreover, most chemical methods require a complicated sample preparation step. Commonly, cleanup methods are based on solid-phase extraction (Furusawa, 1999;Cherlet et al, 2003;Cliquet et al, 2003) or metal chelate affinity chromatography (Croubels et al, 1997). These methods are time-consuming and costly, and require large sample volumes.…”

Binding constants of oxytetracycline to animal feed divalent cations

“…Its usage may result in residues in food products of animal origin, often due to the improper observance of withdrawal periods. For this reason, OTC is often determined in those foods; for example, some authors found this antibiotic in honey (Salinas, Berzas, & Espinosa, 1989;Xie, Dong, Fen, & Liu, 1997), in milk (Boatto, Pau, Palomba, Arenare, & Cerri, 1999;Carson & Breslyn, 1996;Carson, Ngoh, & Hadley, 1998;Furusawa, 1999a;Pena, Lino, & Silveira, 1999;Tjørnelund & Hansen, 1997), in eggs (Coopper et al, 1998;Croubels, Vanoosthuyze, & Van Peteghem, 1997;Furusawa, 1999bFurusawa, , 1999cOmija, Mitema, & Maitho, 1994), and in different animal tissues (Carson et al, 1998;Coopper et al, 1998;Croubels et al, 1997;De Wasch et al, 1998;Farrington et al, 1991;McCracken et al, 1995; Pouliquen et al, 1992;Sokol & Matisova, 1994;Stubbings et al, 1996;Walsh, Walker, & Webber, 1992). OTC has been measured too in other matrices different from food, like plasma (Iwaki, Okumura, & Yamazaki, 1993), urine (Sharma, Koritz, Perkins, & Bevill, 1977;Weimann & Bojesen, 1999;Xie et al, 1997), feeds (Fernández-González, García-Falcó n, & Simal-Gándara, 2002;Hasselberger, 1993;Houglum, Larson, Mutchler, & Wetzler, 1998;Markakis, 1996;Martínez & Shimoda, 1988;Weng, Sun, Roets, & Hoogmartens, 2003)...…”

Development and validation of analytical method for sulfonamide residues in eggs by liquid chromatography Tandem Mass Spectrometry based on the Comission Decision 2002/657/EC

“…3, 2011 technique of analysis. There are methods that use high performance liquid chromatography (HPLC) with fluorescence detector, [2][3][4][5][6] or with ultra-violet (UV) detector, [7][8][9][10] or else with diode array detector (DAD), [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] but mass spectrometer (MS) detectors are the most appropriate because they give enough information for molecular identification.…”