Antibiotic Minimal Selective Concentrations and Fitness Costs during Biofilm and Planktonic Growth

Hjort, Karin; Fermér, Elin; Tang, Po-Cheng; Andersson, Dan I.

doi:10.1128/mbio.01447-22

Cited by 7 publications

(10 citation statements)

References 48 publications

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“…Evolved biofilm populations maintain a higher diversity than corresponding planktonic populations, in which successful mutations reach fixation quickly, and the biofilm environment may protect against negative selection of less fit resistant mutants that would be quickly outcompeted in planktonic cultures 121 . However, a recent study indicated that fitness costs for resistance in surfaceassociated E. coli biofilms did not differ from those in planktonic cultures 79 . In addition, another recent study has shown that the specific environment co-determines fitness and resistance levels associated with specific mutations 131 .…”

Section: Looking At Biofilm Antimicrobial Susceptibility Through the ...mentioning

confidence: 93%

“…There is no information on which concentration range constitutes the mutant selection window (MSW, Box 1 ) for biofilms and various aspects of biofilm biology likely affect this window 90 , 91 . While it has been predicted that biofilm growth leads to shifts and distortions of the MSW 91 , recent work with E. coli showed that minimal selective concentration values (for five different antibiotics) did not differ between planktonic cultures and biofilms 79 . Due to the uncertainty concerning the biofilm MSW, antibiotic concentrations are often selected based on the MIC 70 or on the minimal biofilm inhibitory concentration 74 .…”

Section: Tools To Study Experimental Evolution In Biofilmsmentioning

confidence: 97%

“… Minimal inhibitory concentration (MIC) : lowest concentration of an antibiotic that prevents growth of planktonic cells 31 , 32 . Mutant selection window (MSW) : the concentration range where the fitness of a resistant mutant is higher than the wild type 79 , 91 . Persistence : phenomenon in which at least two subpopulations are present in a population, one consisting of cells that are killed fast by the antibiotic and the other composed of tolerant cells that survive 48 .…”

Section: Biofilms Are Structured Microenvironments—why Does That Matt...mentioning

confidence: 99%

“…Mutant selection window (MSW) : the concentration range where the fitness of a resistant mutant is higher than the wild type 79 , 91 .…”

Section: Biofilms Are Structured Microenvironments—why Does That Matt...mentioning

confidence: 99%

“…The resulting cell suspensions can then be used to start biofilm formation on pegs on a new lid 77 . A conceptually-similar system (FlexiPeg) was recently developed and used to study competition and fitness in biofilms 78 , 79 .…”

Section: Tools To Study Experimental Evolution In Biofilmsmentioning

confidence: 99%

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Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Coenye

Bové

Bjarnsholt

2022

npj Biofilms Microbiomes

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Experimental evolution experiments in which bacterial populations are repeatedly exposed to an antimicrobial treatment, and examination of the genotype and phenotype of the resulting evolved bacteria, can help shed light on mechanisms behind reduced susceptibility. In this review we present an overview of why it is important to include biofilms in experimental evolution, which approaches are available to study experimental evolution in biofilms and what experimental evolution has taught us about tolerance and resistance in biofilms. Finally, we present an emerging consensus view on biofilm antimicrobial susceptibility supported by data obtained during experimental evolution studies.

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Section: Looking At Biofilm Antimicrobial Susceptibility Through the ...mentioning

confidence: 93%

Section: Tools To Study Experimental Evolution In Biofilmsmentioning

confidence: 97%

Section: Biofilms Are Structured Microenvironments—why Does That Matt...mentioning

confidence: 99%

“…Mutant selection window (MSW) : the concentration range where the fitness of a resistant mutant is higher than the wild type 79 , 91 .…”

Section: Biofilms Are Structured Microenvironments—why Does That Matt...mentioning

confidence: 99%

Section: Tools To Study Experimental Evolution In Biofilmsmentioning

confidence: 99%

See 3 more Smart Citations

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Coenye

Bové

Bjarnsholt

2022

npj Biofilms Microbiomes

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Does Environmental Exposure to Pharmaceutical and Personal Care Product Residues Result in the Selection of Antimicrobial‐Resistant Microorganisms, and is this Important in Terms of Human Health Outcomes?

Stanton

Tipper

Chau

et al. 2022

Enviro Toxic and Chemistry

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The environment plays a critical role in the development, dissemination, and transmission of antimicrobial resistance (AMR). Pharmaceuticals and personal care products (PPCPs) enter the environment through direct application to the environment and through anthropogenic pollution. Although there is a growing body of evidence defining minimal selective concentrations (MSCs) of antibiotics and the role antibiotics play in horizontal gene transfer (HGT), there is limited evidence on the role of non-antibiotic PPCPs. Existing data show associations with the development of resistance or effects on bacterial growth rather than calculating selective endpoints. Research has focused on laboratory-based systems rather than in situ experiments, although PPCP concentrations found throughout wastewater, natural water, and soil environments are often within the range of laboratory-derived MSCs and at concentrations shown to promote HGT. Increased selection and HGT of AMR by PPCPs will result in an increase in total AMR abundance in the environment, increasing the risk of exposure and potential transmission of environmental AMR to humans. There is some evidence to suggest that humans can acquire resistance from environmental settings, with water environments being the most frequently studied. However, because this is currently limited, we recommend that more evidence be gathered to understand the risk the environment plays in regard to human health. In addition, we recommend that future research efforts focus on MSC-based experiments for non-antibiotic PPCPS, particularly in situ, and investigate the effect of PPCP mixtures on AMR. Environ Toxicol Chem 2022;00:1-14.

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Highlighting the limitations of static microplate biofilm assays for industrial biocide effectiveness compared to dynamic flow conditions

Klopper,

Bester,

van Schalkwyk

et al. 2023

Environ Microbiol Rep

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The minimal inhibitory concentration of an antimicrobial required to inhibit the growth of planktonic populations (minimum inhibitory concentration [MIC]) remains the ‘gold standard’ even though biofilms are acknowledged to be recalcitrant to concentrations that greatly exceed the MIC. As a result, most studies focus on biofilm tolerance to high antimicrobial concentrations, whereas the effect of environmentally relevant sub‐MIC on biofilms is neglected. The effect of the MIC and sub‐MIC of an isothiazolinone biocide on a microbial community isolated from an industrial cooling system was assessed under static and flow conditions. The differential response of planktonic and sessile populations to these biocide concentrations was discerned by modifying the broth microdilution assay. However, the end‐point analysis of biofilms cultivated in static microplates obscured the effect of sub‐MIC and MIC on biofilms. A transition from batch to the continuous flow system revealed a more nuanced response of biofilms to these biocide concentrations, where biofilm‐derived planktonic cell production was maintained despite an increase in the frequency and extent of biofilm sloughing. A holistic, ‘best of both worlds’ approach that combines the use of static and continuous flow systems is useful to investigate the potential for the development of persistent biofilms under conditions where exposure to sub‐MIC and MIC may occur.

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Antibiotic Minimal Selective Concentrations and Fitness Costs during Biofilm and Planktonic Growth

Cited by 7 publications

References 48 publications

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Does Environmental Exposure to Pharmaceutical and Personal Care Product Residues Result in the Selection of Antimicrobial‐Resistant Microorganisms, and is this Important in Terms of Human Health Outcomes?

Highlighting the limitations of static microplate biofilm assays for industrial biocide effectiveness compared to dynamic flow conditions

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