Antibiotic and Heavy Metal Resistance of Aeromonas spp. Isolated from Diseased Red Hybrid Tilapia (Oreochromis sp.)

Wei, Lee Seong; Mustakim, Maimunah; Azlina, I. Noor; Zulhisyam, A. K.; An’amt, M.N.; Wee, Wendy; Huang, N. M.

doi:10.9734/arrb/2015/15035

Cited by 8 publications

(4 citation statements)

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“…In addition, Aeromonas spp. isolated from red hybrid tilapia showed Cr (35·8%), Cu (25%) and Hg (30%) resistance (Wei et al ). The czcA protein is one of the major components that comprise the chemiosmotic antiporter efflux system which actively passages the cations out of the cell.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic and heavy metal resistance genes inAeromonasspp. isolated from marketed Manila Clam (Ruditapes philippinarum) in Korea

et al. 2019

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Aims: Manila clam (Ruditapes philippinarum) is one of the most popular seafood in Korea, owing to their unique taste and nutritional value. This study aimed to disclose the antibiotic and heavy metal resistance characteristics of Aeromonas spp. isolated from marketed Manila clam in Korea. Methods and results: A total of 36 Aeromonas spp. strains were isolated and subjected to two tests: an antibiotic disk diffusion test to determine their resistance to antibiotics, and a broth dilution test to determine their resistance to heavy metals. PCR-based amplification was performed to detect the resistance genes. A high level of resistance to ampicillin (100%) and cephalothin (89%) was observed, while 42, 39, 36 and 36% of the isolates were resistant to oxytetracycline, imipenem, nalidixic acid and tetracycline respectively. In addition, among the tested heavy metals, cadmium (Cd) recorded the highest resistance rate (61%), followed by chromium (Cr) (50%), lead (Pb) (47%) and copper (Cu) (37%). However, mercury (Hg) resistance was not observed. PCRs revealed the occurrence of bla TEM , bla SHV , bla CTX-M , qnrS, tetB, tetE, aac(6 0 )-Ib, strA-strB and intI1 genes among 100, 31, 31, 78, 78, 89, 25, 50 and 72% of the isolates respectively. Moreover, heavy metal resistance genes, copA, merA and czcA were detected in 25, 47 and 61% of the isolates respectively. Conclusions: The results suggest the importance of multi-drug and heavy metal-resistant aeromonads in Manila clam to assess the consumer safety and public health. Significance and Impact of the Study: This study is the first to elaborate on the importance of multi-drug and heavy metal-resistant aeromonads in Manila clam. Particularly, the presence of extended-spectrum-b-lactamase genes and other antibiotic resistance genes intensifies the possible health risks and may complicate therapeutic treatments upon infection, while heavy metal resistance suggests possible heavy metal exposure.

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Section: Discussionmentioning

confidence: 99%

Antibiotic and heavy metal resistance genes inAeromonasspp. isolated from marketed Manila Clam (Ruditapes philippinarum) in Korea

et al. 2019

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“…They are cosmopolitan in distribution. Mesophilic motile aeromonads are ubiquitous and autochthonous aquatic microorganisms occurring in fresh water, sewage and brackish water [ 6 ] and in chlorinated and unchlorinated drinking water [ 7 , 8 ]. Besides these aeromonads occur as the normal microbial flora of many aquatic and terrestrial animals and are proven diseases in various cold blooded and warm blooded animals including fishes, amphibians, reptiles, birds and other domestic animals [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review

Praveen¹,

Debnath²,

Shekhar

et al. 2016

Vet World

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Aeromonas is recognized to cause a variety of diseases in man. In humans, they are associated with intestinal and extra-intestinal infections. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonas strains may produce many different putative virulence factors such as enterotoxins, hemolysins or cytotoxins, and antibiotic resistance against different antibiotics. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. Comprehensive enteric disease surveillance strategies, prevention and education are essential for meeting the challenges in the years ahead. It is important for us to promote the value of enteric cultures when patients have a gastrointestinal illness or bloody diarrhea or when multiple cases of enteric disease occur after a common exposure. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonas strains may produce many different putative virulence factors, such as enterotoxins, hemolysins or cytotoxins. It has been established that aerolysin is a virulence factor contributing to the pathogenesis of Aeromonas hydrophila infection. Fish and chicken play an important role in the transmission of this pathogen to humans. In the present study, the high prevalence of toxin-producing strains was found among the Aeromonas isolates. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. The present review was constructed with a view to highlight the zoonotic importance of Aeromonas pathogen in fish and chicken meat.

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“…among farmed fish and imported pet fish in Korea. Moreover, Wei et al (2015) demonstrated that 25% of Aeromonas spp. isolated from red hybrid tilapia exhibited resistance to Cu.…”

Section: Discussionmentioning

confidence: 99%

Dual resistance to heavy metals and antibiotics of Aeromonas hydrophila isolated from Carassius carassius (Linnaeus, 1758) in Lake Tonga, Algeria

Benhalima,

Amri,

Bensouilah

2023

Studia Universitatis Babeş-Bolyai Biologia

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Aeromonas hydrophila, a bacterium with significant virulence potential, is the predominant pathogenic bacteria naturally infecting fish. This study aims to identify the antibiogram and heavy metal resistance pattern of Aeromonas hydrophila obtained from both Carassius carassius fish and their surrounding water environment in Lake Tonga, Algeria. A total of 59 strains of Aeromonas hydrophila were isolated from 168 Carassius carassius samples and 144 waters samples of Lake Tonga. All the strains were tested for resistance to 13 antibiotics and three types of heavy metals (Cobalt, copper and cadmium) using disk diffusion and two-fold agar dilution method, respectively. Clinical macroscopic examination of the fish was also carried out. More than 14% of the examined fishes showed the characteristic clinical signs. Drug screening showed high levels of resistance to β-lactam antibiotics, 100% of the strains were resistant to ampicillin followed by cefalotin (91.53%) and ticarcillin (88.14%). More than 40% of the strains exhibited resistance against gentamicin, amikacin and chloramphenicol. The multiple antibiotic resistance (MAR) indexing of A. hydrophila strains showed that all of them originated from high-risk sources. Among tested heavy metals, bacterial isolates exhibited resistant pattern of Co>Cu >Cd. A positive correlation was observed between antimicrobial resistance and metal tolerance (Odds Ratio>0.1). These resistant profiles could be useful information to avoid unnecessary use of chemical and antimicrobial products in the aquatic environment and to provide a novel approach to manage bacterial infection in fish.

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Antibiotic and Heavy Metal Resistance of Aeromonas spp. Isolated from Diseased Red Hybrid Tilapia (Oreochromis sp.)

Cited by 8 publications

References 0 publications

Antibiotic and heavy metal resistance genes inAeromonasspp. isolated from marketed Manila Clam (Ruditapes philippinarum) in Korea

Antibiotic and heavy metal resistance genes inAeromonasspp. isolated from marketed Manila Clam (Ruditapes philippinarum) in Korea

Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review

Dual resistance to heavy metals and antibiotics of Aeromonas hydrophila isolated from Carassius carassius (Linnaeus, 1758) in Lake Tonga, Algeria

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