Antibiotic administration alleviates the aggravating effect of orthodontic force on ligature‐induced experimental periodontitis bone loss in mice

Shi, Jie; Liu, Zhiqiang; Kawai, Toshihisa; Zhou, Yanheng; Han, Xiaozhe

doi:10.1111/jre.12439

Cited by 12 publications

(15 citation statements)

References 41 publications

(43 reference statements)

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“…This study aimed to evaluate the efficacy of the novel test compound OAA on healing and improved quality of bone formation in and around the socket region retained after periodontitis and as a consequence of tooth loss by local delivery into the affected site. To evaluate the quality of bone healing after drug treatment, we used the modified ligature‐induced periodontitis model system . After 5 days of ligation, bone loss was significantly higher in the buccal region compared to the palatal region in our experiment.…”

Section: Discussionmentioning

confidence: 86%

“…To induce periodontitis, anesthetized mice were ligated with black silk 5-0 (SK54510, AILEE CO, LTD, Korea) around the maxillary left second molar with a slight modification of the previously described procedure. 9,22 The animals were anesthetized by an intraperitoneal injection of Avertin (Sigma-Aldrich, USA) as previously described. 23 Four mice with ligature were sacrificed to harvest maxilla with an intact palate.…”

Section: Periodontitis Model System and Evaluationmentioning

confidence: 99%

“…To evaluate the quality of bone healing after drug treatment, we used the modified ligature-induced periodontitis model system. 9,22 After 5 days of ligation, bone loss was significantly higher in the buccal while the volume of bone formation was evaluated by micro-CT at 2 weeks with modifications based on previous reports. 30,31 As alveolar bone loss was high in the buccal region, we analyzed bone formation levels in the buccal region of the tooth root socket.…”

Section: Experimental Periodontitis Model System and Drug Treatmentmentioning

confidence: 99%

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Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Adhikari

Neupane

Aryal

et al. 2019

J of Periodontal Research

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Objective We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis. Background Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss. Methods Maxillary left second molar was extracted from 8‐week‐old male mice following induction of periodontitis by ligature for 5 days. The extraction socket was treated with 50 ng/µL OAA for 1, 2, and 3 weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4/80, interleukin (IL)‐6, and osteocalcin, were observed. The volume of bone formation was examined by Micro‐CT. Osteoclasts were enumerated using tartrate‐resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging‐drop in vitro cultivation method at E14 for 1 day and examined the expression pattern of transforming growth factor (TGF)‐β superfamily and Wnt signaling genes. Results Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA‐treated specimens showed the altered localization patterns of inflammatory and bone formation‐related signaling molecules including CD31, F4/80, IL‐6, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)‐β superfamily and Wnt signaling. Conclusions Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF‐β and Wnt signaling.

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Section: Discussionmentioning

confidence: 86%

Section: Periodontitis Model System and Evaluationmentioning

confidence: 99%

Section: Experimental Periodontitis Model System and Drug Treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Adhikari

Neupane

Aryal

et al. 2019

J of Periodontal Research

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“…In addition we checked the previous literature and found typical sample sizes of 5–8 animals per group. 45 , 55 – 57 For our study we had a sample size of 7–8 animals per group for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

RANKL deletion in periodontal ligament and bone lining cells blocks orthodontic tooth movement

et al. 2018

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The bone remodeling process in response to orthodontic forces requires the activity of osteoclasts to allow teeth to move in the direction of the force applied. Receptor activator of nuclear factor-κB ligand (RANKL) is essential for this process although its cellular source in response to orthodontic forces has not been determined. Orthodontic tooth movement is considered to be an aseptic inflammatory process that is stimulated by leukocytes including T and B lymphocytes which are presumed to stimulate bone resorption. We determined whether periodontal ligament and bone lining cells were an essential source of RANKL by tamoxifen induced deletion of RANKL in which Cre recombinase was driven by a 3.2 kb reporter element of the Col1α1 gene in experimental mice (Col1α1.CreERTM+.RANKLf/f) and compared results with littermate controls (Col1α1.CreERTM−.RANKLf/f). By examination of Col1α1.CreERTM+.ROSA26 reporter mice we showed tissue specificity of tamoxifen induced Cre recombinase predominantly in the periodontal ligament and bone lining cells. Surprisingly we found that most of the orthodontic tooth movement and formation of osteoclasts was blocked in the experimental mice, which also had a reduced periodontal ligament space. Thus, we demonstrate for the first time that RANKL produced by periodontal ligament and bone lining cells provide the major driving force for tooth movement and osteoclastogenesis in response to orthodontic forces.

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“…All protocols described below were approved by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University. Bilateral maxillary first molars of each animal were selected to undergo orthodontic ligature in the submarginal position to induce experimental periodontitis [29]. A mix of 10 9 colony-forming units (CFU) of each periodontal pathogen, such as Porphyromonas gingivalis (Pg, ATCC 33277) and Fusobacterium nucleatum (Fn, ATCC 25586), was applied on the surface of the maxillary first molar teeth according to a previous study [30].…”

Section: Methodsmentioning

confidence: 99%

Methylene Blue-Mediated Photodynamic Therapy Induces Macrophage Apoptosis via ROS and Reduces Bone Resorption in Periodontitis

Jiang

Yang

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Aim. To investigate whether methylene blue-mediated photodynamic therapy (MB-PDT) can affect the “fate” of macrophages in vitro or in periodontitis tissues and to explore the potential mechanism. Methods. For in vitro treatments, THP-1 macrophages were divided into three experimental groups: C/control, no treatment; MB, methylene blue treatment; and MB-PDT, MB and laser irradiation treatment. Then, apoptosis and apoptosis-related proteins were detected in each group. For in vivo treatments, periodontitis was ligature-induced in the first molars of the bilateral maxilla in 12 Sprague Dawley (SD) rats. After six weeks, the ligatures were removed and all the induced molars underwent scaling and root planning (SRP). Then, the rats were divided into three groups according to the following treatments: SRP, saline solution; MB, phenothiazinium dye; and MB-PDT, MB and laser irradiation. Apoptotic macrophages, inflammation levels, and alveolar bone resorption in the periodontal tissues of rats were analyzed in each group. Results. In vitro, flow cytometry analysis demonstrated that 10 μM MB and 40 J/cm2 laser irradiation maximized the apoptosis rate (34.74%) in macrophages. Fluorescence probe and Western blot analyses showed that MB-PDT induced macrophage apoptosis via reactive oxygen species (ROS) and the mitochondrial-dependent apoptotic pathway. Conversely, the addition of exogenous antioxidant glutathione (GSH) and the pan-caspase inhibitor Z-VAD-FMK markedly reduced the apoptotic response in macrophages. In vivo, immunohistochemistry, histology, radiographic, and molecular biology experiments revealed fewer infiltrated macrophages, less bone loss, and lower IL-1β and TNF-α levels in the MB-PDT group than in the SRP and MB groups (P<0.05). Immunohistochemistry analysis also detected apoptotic macrophages in the MB-PDT group. Conclusion. MB-PDT could induce macrophage apoptosis in vitro and in rats with periodontitis. This may be another way for MB-PDT to relieve periodontitis in addition to its antimicrobial effect. Meanwhile, MB-PDT induced apoptosis in THP-1 macrophages via the mitochondrial caspase pathway.

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Antibiotic administration alleviates the aggravating effect of orthodontic force on ligature‐induced experimental periodontitis bone loss in mice

Cited by 12 publications

References 41 publications

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

RANKL deletion in periodontal ligament and bone lining cells blocks orthodontic tooth movement

Methylene Blue-Mediated Photodynamic Therapy Induces Macrophage Apoptosis via ROS and Reduces Bone Resorption in Periodontitis

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