Antibiotic activity and synergistic effect of antimicrobial peptide against pathogens from a patient with gallstones

Park, Yoonkyung; Park, Soon Nang; Park, Seong-Cheol; Park, Joon Yong; Park, Yong Ha; Hahm, Joon Soo; Hahm, Kyung‐Soo

doi:10.1016/j.bbrc.2004.07.008

Cited by 19 publications

(12 citation statements)

References 34 publications

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“…The hemolytic activity of pyocyanin was evaluated by measuring the release of hemoglobin from fresh human erythrocytes as per Park et al ( 2004 ). L929 fibroblast cell lines (NCCS, Pune, India) was maintained in Dulbecco’s modified Eagles medium (HiMedia) supplemented with 10 % FBS (Invitrogen) and grown to confluence at 37 °C in 5 % CO 2 in humidified atmosphere of CO 2 incubator (NBS, Eppendorf, Germany).…”

Section: Methodsmentioning

confidence: 99%

Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Laxmi

Bhat

2016

3 Biotech

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A biomolecule namely pyocyanin was isolated from Pseudomonas aeruginosa (BTRY1). It was confirmed by UV–Vis spectrum with absorption maxima at 270 nm and was further characterized using Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR). Spectroscopy analyses showed the presence of relevant bonds in their respective structures. Although pyocyanin had antihemolytic, antioxidant and antibiofilm activities against food pathogens, it showed no observable cytotoxicity, and thus proposed for potential use in the preservation of food.

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Section: Methodsmentioning

confidence: 99%

Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Laxmi

Bhat

2016

3 Biotech

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“…Hemolytic activity was evaluated turbidimetrically, as described previously (Park et al, 2004). Erythrocyte suspensions were prepared using fresh rabbit red blood cells (RBCs), washed and resuspended in Tris-buffered saline (TBS, 145 mM NaCl, 10 mM Tris-HCl, pH 7.4).…”

Section: Hemolytic Activitymentioning

confidence: 99%

Discovery of a novel antimicrobial peptide using membrane binding-based approach

et al. 2009

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“…The percentage hemolysis was calculated using the following equation: % hemolysis = [( A 414 nm with peptide solution − A 414 nm in PBS)/( A 414 nm with 0.1% Triton X‐100 − A 414 nm in PBS)]× 100. Each measurement was made in triplicate27. The hemolytic peptide melittin was synthesized in the Chosun University laboratories.…”

Section: Suv Preparationmentioning

confidence: 99%

Synthesis, preferred conformation, protease stability, and membrane activity of heptaibin, a medium‐length peptaibiotic

Zotti

Biondi

Peggion

et al. 2011

Journal of Peptide Science

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The medium-length peptaibiotics are characterized by a primary structure of 14-16 amino acid residues. Despite the interesting antibiotic and antifungal properties exhibited by these membrane-active peptides, their exact mechanism of action is still unknown. Here, we present our results on heptaibin, a 14-amino acid peptaibiotic found to exhibit antimicrobial activity against Staphylococcus aureus. We carried out the very challenging synthesis of heptaibin on solid phase and a detailed conformational analysis in solution. The peptaibiotic is folded in a mixed 3₁₀-/α-helix conformation which exhibits a remarkable amphiphilic character. We also find that it is highly stable toward degradation by proteolytic enzymes and nonhemolytic. Finally, fluorescence leakage experiments using small unilamellar vesicles of three different compositions revealed that heptaibin, although uncharged, is a selective compound for permeabilization of model membranes mimicking the overall negatively charged surface of Gram-positive bacteria. This latter finding is in agreement with the originally published antimicrobial activity data.

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Antibiotic activity and synergistic effect of antimicrobial peptide against pathogens from a patient with gallstones

Cited by 19 publications

References 34 publications

Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Discovery of a novel antimicrobial peptide using membrane binding-based approach

Synthesis, preferred conformation, protease stability, and membrane activity of heptaibin, a medium‐length peptaibiotic

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