Antibiogram of the Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus intermedius isolated from the bovine frozen semen

Abro, Shahid Hussain; Abro, Rani; Rind, Rahmatullah; Kamboh, Asghar Ali; Memon, Akeel Ahmed; Baloch, H.; Wagan, Bakhtawar

doi:10.19045/bspab.2016.50027

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…The resistance of P. aeruginosa to chloramphenicol is usually intrinsically, in part due to the MexAB-OprM efflux system [48]. In addition, all isolates exhibit a full resistance (100%) to erythromycin, this results agree with a study in India who detect (100%) resistance from clinical isolate [49], and disagree with result obtained by a study [50] who found (60.50%) of isolates were sensitive to erythromycin.…”

Section: For Fluoroquinolone Classsupporting

confidence: 63%

Prevalence and Antibiogram Patterns of Pseudomonas aeruginosa Isolated from Two Wastewater Treatment Plants in Aden Governorate-Yemen

Al-Qashbari,

Al-Baghdadi

2024

YJMS

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Introduction: Pseudomonas aeruginosa is a gram-negative rod-shaped obligatorily aerobic bacterium. This bacterium is most frequently associated with human infections and is regarded as an opportunistic pathogen, primarily causing nosocomial infections in immunocompromised patients. P. aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water as well as in other sources such as sewage treatment plants could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Therefore, the aim of this study was to determine the prevalence, antibiogram pattern and the multiple antibiotics resistant (MAR) of P. aeruginosa. Methods: The study was designed as a cross-sectional study, conducted between September 2022 and February 2023. A total of 193 wastewater samples, (97/193) & (96/193) were obtained from two region AL-Magari and Kaputa wastewater treatment plants (WWTPs), respectively. The wastewater samples were serially diluted and cultured on enrichment medium, then cultured on cetrimide agar as a selective medium for P. aeruginosa. The isolates were identified primarily based on cultural characteristics and then by biochemical tests. A panel of 32 antibiotics were used to determine the susceptibility patterns and the MAR. Results: The prevalence of P. aeruginosa was 33.7 % (65/193), while the statistical data revealed no a statistically significant difference between the prevalence of P. aeruginosa isolates from AL-Magari and Kaputa (P˃0.05). The study revealed the presence of antipseudomonal agents among the wastewater isolates of P. aeruginosa. Ciprofloxacin antibiotic has the highest activity against P. aeruginosa strains with the lowest resistant rate (7.7%), followed by Levofloxacin, Ofloxacin and Piperacillin/Tazobactam, (12.3%), (18.5%), and (20%), respectively. The MAR index ranged between 0.562 to 1. Conclusion: The study demonstrated that MAR P. aeruginosa were quite prevalent in the wastewater effluents of WWTPs in Aden governorate-Yemen with no statistically significant difference in resistant pattern between wastewater isolates from AL-Magari and Kaputa region to the antibiotics used in this study (P˃0.05); and this can lead to serious health risk for community, and natural vegetation where plants grow.

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Section: For Fluoroquinolone Classsupporting

confidence: 63%

Prevalence and Antibiogram Patterns of Pseudomonas aeruginosa Isolated from Two Wastewater Treatment Plants in Aden Governorate-Yemen

Al-Qashbari,

Al-Baghdadi

2024

YJMS

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“…Numerous bacterial agents have been isolated worldwide from semen specimens of cattle and other domestic animals. Recently, bacterial organisms such as Micrococcus luteus, Pseudomonas aeruginosa, S. epidermidis and Staphylococcus intermedius have been detected in frozen bovine semen (Abro et al, 2016). Sannat et al (2015) described bacterial loads found in fresh and frozen semen from different breeds of cattle and buffalo.…”

Section: Introductionmentioning

confidence: 99%

Escherichia fergusonii identified in preputial swabs from healthy Aceh cattle by phylogenetic 16S rRNA analysis

Balqis¹,

Hambal²,

Admi³

et al. 2018

MJM

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Aims: This study aimed to assess the risk of reproductive tract contamination in Aceh cattle by Escherichia fergusonii as revealed by 16S rRNA gene sequencing of preputial swab samples. Methodology and results: Preputial swabs taken from 50 breeding bulls at the Indrapuri Breeding and Forage Center of Aceh Cattle, Banda Aceh, Indonesia, were examined for the presence of bacteria. Samples were streaked on MacConkey agar and incubated under aerobic conditions at 37 °C for 24 h. Smooth, yellow-or rose-colored colonies were selected for their characteristic appearance and subjected to further analysis. Genetic identification was based on 16S rRNA gene sequencing and PCR analysis. We conducted a 16S rRNA sequence similarity search with GenBank using BLAST and constructed neighbour-joining dendrograms using MEGA. From among closely related species of the genus Enterobacteriaceae, we identified the enteric bacterium E. fergusonii as having the highest sequence similarity. Conclusion, significance and impact of study: We concluded that the E. fergusonii bacterium positively presence in preputial swab samples of clinically healthy Aceh cattle population. Accordingly, it is potentially allowing the bacterium to be spread during natural mating or semen collection processing for artificial insemination in cattle breeding farm.

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“… Common clinical group Genus or Species No. Estimated % amoxicillin susceptibility for group [ [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] ] % Total for group Aerobic gram-positive organisms Staphylococcus aureus Staphylococcus aureus 5 0 11.4 Pyogenic streptococci Group C Streptococcus 1 100 4.5 Group G Streptococcus 1 Viridans group streptococci S. salivarius 1 50 38.6 S. anginosus group ( S. intermedius ) 1 S. mitis group 2 S. oralis 1 S. gordonii 1 S. mutans 1 viridans group Streptococcus , NOS 10 Formally nutritionally variant streptococci Granulicatella adiacens 2 50 4.5 Micrococcaceae Rothia dentocariosa 1 60 11.4 Rothia mucilaginosa 1 Rothia aeria 1 Rothia sp. 1 Micrococcus sp.…”

Section: Resultsmentioning

confidence: 99%

“…The organisms reported were sorted by common clinical grouping, family, and genus. The susceptibilities of group or family or genus were then estimated by one author, a specialist of infectious diseases, using the John Hopkins Antibiotic Guide, the Sanford Guide to Antimicrobial Therapy, and a literature review for rare genera [ [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] ]. An overall estimated amoxicillin susceptibility rate was determined from a weighted mean calculated using the frequency of organisms and the estimated amoxicillin susceptibility by genus or species.…”

Section: Methodsmentioning

confidence: 99%

Prosthetic Joint Infection After Dental Work: Is the Correct Prophylaxis Being Prescribed? A Systematic Review

et al. 2021

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Background: Prosthetic joint infection (PJI) of total hip (THA) or total knee arthroplasty (TKA) after dental procedures is uncommon, and antibiotic prophylaxis remains controversial. For high-risk patients, the American Academy of Orthopedic Surgeons recommends amoxicillin prophylaxis. However, no systematic review of the literature of PJIs associated with dental procedures explores if amoxicillin is suitable for the reported organisms. Methods: A librarian-assisted search of the major databases (PubMed, Medline, Embase, Scopus) identified 954 articles. Only case reports, case series, and reviews with patient level data were included. After exclusions, 79 articles were fully reviewed. Results: Forty-four PJIs after dental procedures were identified, 22 in primary THA, 20 in primary TKA, one in revision THA, and one in a hip resurfacing procedure. Antibiotic prophylaxis was documented for 5 patients. The dental procedure was invasive in 35 (79.5%). Comorbidities were present in 17 patients (38.7%). The organisms reported were Streptococcus spp. in 44%, other aerobic gram-positives in 27%, anaerobic gram-positives in 18%, and gram-negative organisms in 11%. An estimated 46% of organisms may be resistant to amoxicillin. The outcomes of treatment were reported for 35 patients (79.5%). Twenty-seven patients (61.4%) had no clinical signs of PJI at the final follow-up visit. Conclusions: Lower extremity PJI associated with dental procedures is often caused by organisms unlikely to be prevented with amoxicillin. Additional studies are warranted to determine the choice and efficacy of antibiotic prophylaxis to prevent dental-associated PJI in the highest risk patients. Insufficient data exist to recommend the optimal treatment for patients with PJI in THA and TKA associated with dental procedures.

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Antibiogram of the Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus intermedius isolated from the bovine frozen semen

Cited by 4 publications

References 26 publications

Prevalence and Antibiogram Patterns of Pseudomonas aeruginosa Isolated from Two Wastewater Treatment Plants in Aden Governorate-Yemen

Prevalence and Antibiogram Patterns of Pseudomonas aeruginosa Isolated from Two Wastewater Treatment Plants in Aden Governorate-Yemen

Escherichia fergusonii identified in preputial swabs from healthy Aceh cattle by phylogenetic 16S rRNA analysis

Prosthetic Joint Infection After Dental Work: Is the Correct Prophylaxis Being Prescribed? A Systematic Review

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